RHOB siRNA – Rho-Related GTP-Binding Protein Rhob (Mouse)

Name: RHOB siRNA; Rho-Related GTP-Binding Protein Rhob; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS8232976
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8232976

MBS8232976 is an siRNA targeting RHOB. Also known as RHOB siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit RHOB expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

RHOB, siRNA

Full Product Name

RHOB siRNA (Mouse)

Product Gene Name

RHOB sirna

Product Synonym Names

ARHB; Rho-related GTP-binding protein RhoB

Host

Synthetic

Species Reactivity

Mouse

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

RHOB siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

22,123 Da

3D Structure

ModBase 3D Structure for P62746

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RHOB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for RHOB sirna

RNA Interference (RNAi)

Other Notes

Small volumes of RHOB sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RHOB. It may not necessarily be applicable to this product.

NCBI GI #

6680726

NCBI GeneID

11852

NCBI Accession #

NP_031509.1

UniProt Primary Accession #

P62746

UniProt Secondary Accession #

P01121; Q9CUV7

UniProt Related Accession #

P62746

GenBank Nucleotide #

NM_007483.2

NCBI Official Full Name

rho-related GTP-binding protein RhoB

NCBI Official Synonym Full Names

ras homolog gene family, member B

NCBI Official Symbol

Rhob

NCBI Official Synonym Symbols

Arh6; Arhb; AA017882

UniProt Protein Name

Rho-related GTP-binding protein RhoB

Protein Family

Rho-related GTP-binding protein

UniProt Gene Name

Rhob

UniProt Synonym Gene Names

Arhb

UniProt Entry Name

RHOB_MOUSE

NCBI Protein Information

rho-related GTP-binding protein RhoB

UniProt Comments for RHOB

RHOB: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.

Protein type: G protein; Motility/polarity/chemotaxis; Tumor suppressor; G protein, monomeric; G protein, monomeric, Rho

Cellular Component: focal adhesion; membrane; cell; early endosome; late endosome; plasma membrane; endosome membrane; intracellular; cytosol; nucleus; endosome; cleavage furrow

Molecular Function: protein binding; GDP binding; GTP binding; nucleotide binding

Biological Process: transformed cell apoptosis; apoptosis; positive regulation of apoptosis; multicellular organismal development; cytokinesis; negative regulation of cell cycle; intracellular protein transport; protein transport; positive regulation of angiogenesis; transport; small GTPase mediated signal transduction; endosome to lysosome transport; angiogenesis; cell differentiation; cell adhesion

Research Articles on RHOB

1. Active RhoA and RhoB are involved in back end functions, such as retracting the tail and lamellipodial membrane extensions during macrophage migration.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with RHOB sirna

Reference Product	PubMed Publications
Rock1 sirna	>10 publications with RHOB and Rock1
Erbb2 sirna	>8 publications with RHOB and Erbb2
Rock2 sirna	>5 publications with RHOB and Rock2
Arhgdia sirna	>5 publications with RHOB and Arhgdia
Arhgef2 sirna	>3 publications with RHOB and Arhgef2
Arhgdib sirna	>3 publications with RHOB and Arhgdib
Arhgef11 sirna	>1 publications with RHOB and Arhgef11
Ect2 sirna	>1 publications with RHOB and Ect2
Arhgdig sirna	>1 publications with RHOB and Arhgdig

Pathways associated with RHOB sirna

Products by Pathway	Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
G Alpha (12/13) Signalling Events Pathway antibodies	G Alpha (12/13) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPVI-mediated Activation Cascade Pathway antibodies	GPVI-mediated Activation Cascade Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Platelet Activation, Signaling And Aggregation Pathway antibodies	Platelet Activation, Signaling And Aggregation Pathway Diagram
RHO GTPase Effectors Pathway antibodies	RHO GTPase Effectors Pathway Diagram
RHO GTPases Activate Formins Pathway antibodies	RHO GTPases Activate Formins Pathway Diagram

Diseases associated with RHOB sirna

Disease Name	Pubmed Publications
Cell Transformation, Neoplastic Antibodies	>37 publications with RHOB and Cell Transformation, Neoplastic
Breast Neoplasms Antibodies	>25 publications with RHOB and Breast Neoplasms
Lung Neoplasms Antibodies	>22 publications with RHOB and Lung Neoplasms
Neoplasm Metastasis Antibodies	>22 publications with RHOB and Neoplasm Metastasis
Nervous System Diseases Antibodies	>19 publications with RHOB and Nervous System Diseases
Neoplasms, Experimental Antibodies	>14 publications with RHOB and Neoplasms, Experimental
Disease Models, Animal Antibodies	>14 publications with RHOB and Disease Models, Animal
Brain Diseases Antibodies	>14 publications with RHOB and Brain Diseases
Inflammation Antibodies	>13 publications with RHOB and Inflammation
Kidney Diseases Antibodies	>9 publications with RHOB and Kidney Diseases

Organs/Tissues associated with RHOB sirna

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>69 publications with RHOB and Brain
Lung Antibodies	>42 publications with RHOB and Lung
Muscle Antibodies	>39 publications with RHOB and Muscle
Kidney Antibodies	>33 publications with RHOB and Kidney
Liver Antibodies	>27 publications with RHOB and Liver
Bone Antibodies	>26 publications with RHOB and Bone
Heart Antibodies	>18 publications with RHOB and Heart
Skin Antibodies	>13 publications with RHOB and Skin
Prostate Antibodies	>13 publications with RHOB and Prostate
Bone Marrow Antibodies	>12 publications with RHOB and Bone Marrow