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RYR2 siRNA – Ryanodine Receptor 2 (Mouse)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8215453

MBS8215453 is an siRNA targeting RYR2. Also known as RYR2 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit RYR2 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RYR2, siRNA
Full Product Name
RYR2 siRNA (Mouse)
Product Gene Name
RYR2 sirna
Product Synonym Names
Ryanodine receptor 2; RYR-2; RyR2; Cardiac muscle ryanodine receptor; Cardiac muscle ryanodine receptor-calcium release channel; Type 2 ryanodine receptor
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
RYR2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
564,819 Da
3D Structure
ModBase 3D Structure for E9Q401
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RYR2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for RYR2 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of RYR2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RYR2. It may not necessarily be applicable to this product.

NCBI GI #
124430578
NCBI GeneID
20191
NCBI Accession #
NP_076357.2
UniProt Primary Accession #
E9Q401
UniProt Secondary Accession #
O70181; Q62174; Q62197; Q9ERN6
UniProt Related Accession #
E9Q401
GenBank Nucleotide #
NM_023868.2
NCBI Official Full Name
ryanodine receptor 2
NCBI Official Synonym Full Names
ryanodine receptor 2, cardiac
NCBI Official Symbol
Ryr2
NCBI Official Synonym Symbols
RYR-2; 9330127I20Rik
UniProt Protein Name
Ryanodine receptor 2
UniProt Synonym Protein Names
Cardiac muscle ryanodine receptor; Cardiac muscle ryanodine receptor-calcium release channel; Type 2 ryanodine receptor
Protein Family
Ryanodine receptor
UniProt Gene Name
Ryr2
UniProt Synonym Gene Names
RYR-2; RyR2
UniProt Entry Name
RYR2_MOUSE
NCBI Protein Information
ryanodine receptor 2

UniProt Comments for RYR2

RYR2: ryanodine receptor calcium release channel. Regulates communication between transverse-tubules and sarcoplasmic reticulum. Contraction of cardiac muscle is triggered by release of calcium ions from SR following depolarization of T-tubules. Phosphorylation by PKA dissociates FKBP12.6 from RYR2. Two splice isoforms have been described.

Protein type: Membrane protein, multi-pass; Channel, ligand-gated; Membrane protein, integral; Transporter, ion channel; Transporter

Cellular Component: neuron projection; sarcoplasmic reticulum membrane; smooth endoplasmic reticulum; protein complex; sarcoplasmic reticulum; endoplasmic reticulum; cell; integral to membrane; nuclear envelope; cytosol; Z disc; sarcomere; extrinsic to internal side of plasma membrane; membrane; cytoplasm; plasma membrane; intracellular; A band; sarcolemma

Molecular Function: calcium-induced calcium release activity; calmodulin binding; identical protein binding; protein binding; enzyme binding; protein self-association; calcium-release channel activity; calcium channel activity; ion channel activity; calcium ion binding; intracellular ligand-gated calcium channel activity; protein kinase binding; ryanodine-sensitive calcium-release channel activity

Biological Process: positive regulation of sequestering of calcium ion; cytosolic calcium ion homeostasis; regulation of heart rate; multicellular organismal development; calcium-mediated signaling; positive regulation of heart rate; response to redox state; Wnt receptor signaling pathway through beta-catenin; release of sequestered calcium ion by sarcoplasmic reticulum into cytosol; response to caffeine; cellular calcium ion homeostasis; BMP signaling pathway; response to magnesium ion; transport; release of sequestered calcium ion into cytosol; reduction of cytosolic calcium ion concentration; calcium ion transport; response to hypoxia; ion transport; detection of calcium ion; response to calcium ion; transmembrane transport; cardiac muscle contraction
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Research Articles on RYR2

1. analysis of arrhythmia mechanisms in a RyR2-linked CPVT mutation (RyR2-A4860G) that depresses channel activity

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with RYR2 sirna

 Reference Product  PubMed Publications
 Casq2 sirna  >92 publications with RYR2 and Casq2
 Trdn sirna  >46 publications with RYR2 and Trdn
 Cacna1s sirna  >42 publications with RYR2 and Cacna1s
 Cacna1c sirna  >24 publications with RYR2 and Cacna1c
 Nos1 sirna  >14 publications with RYR2 and Nos1
 Cacna1d sirna  >7 publications with RYR2 and Cacna1d
 Fkbp1b sirna  >6 publications with RYR2 and Fkbp1b
 Calm1 sirna  >5 publications with RYR2 and Calm1
 Camk2g sirna  >2 publications with RYR2 and Camk2g
 Pkd2l1 sirna  >1 publications with RYR2 and Pkd2l1

Pathways associated with RYR2 sirna

 Products by Pathway  Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Cardiac Muscle Contraction Pathway antibodies  Cardiac Muscle Contraction Pathway Diagram
 Circadian Entrainment Pathway antibodies  Circadian Entrainment Pathway Diagram

Diseases associated with RYR2 sirna

 Disease Name  Pubmed Publications
 VENTRICULAR TACHYCARDIA, CATECHOLAMINERGIC POLYMORPHIC, 1, WITH OR WITHOUT ATRIAL DYSFUNCTION AND/OR DILATED CARDIOMYOPATHY Antibodies  >19168 publications with RYR2 and VENTRICULAR TACHYCARDIA, CATECHOLAMINERGIC POLYMORPHIC, 1, WITH OR WITHOUT ATRIAL DYSFUNCTION AND/OR DILATED CARDIOMYOPATHY
 Heart Diseases Antibodies  >1010 publications with RYR2 and Heart Diseases
 Arrhythmias, Cardiac Antibodies  >607 publications with RYR2 and Arrhythmias, Cardiac
 Nervous System Diseases Antibodies  >474 publications with RYR2 and Nervous System Diseases
 Heart Failure Antibodies  >465 publications with RYR2 and Heart Failure
 Tachycardia Antibodies  >379 publications with RYR2 and Tachycardia
 Tachycardia, Ventricular Antibodies  >360 publications with RYR2 and Tachycardia, Ventricular
 Disease Models, Animal Antibodies  >248 publications with RYR2 and Disease Models, Animal
 Cardiomyopathies Antibodies  >170 publications with RYR2 and Cardiomyopathies
 Vascular Diseases Antibodies  >157 publications with RYR2 and Vascular Diseases

Organs/Tissues associated with RYR2 sirna

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >2168 publications with RYR2 and Heart
 Brain Antibodies  >594 publications with RYR2 and Brain
 Embryonic Tissue Antibodies  >127 publications with RYR2 and Embryonic Tissue
 Pancreas Antibodies  >85 publications with RYR2 and Pancreas
 Eye Antibodies  >48 publications with RYR2 and Eye
 Uterus Antibodies  >24 publications with RYR2 and Uterus
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