THRB siRNA – Thyroid Hormone Receptor Beta Isoform 1 (Mouse)

Name: THRB siRNA; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS8216621
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8216621

MBS8216621 is an siRNA targeting THRB. Also known as THRB siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit THRB expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

THRB, siRNA

Full Product Name

THRB siRNA (Mouse)

Product Gene Name

THRB sirna

Product Synonym Names

ERBA2; NR1A2; Thyroid hormone receptor beta; Nuclear receptor subfamily 1 group A member 2; c-erbA-2; c-erbA-beta

Host

Synthetic

Species Reactivity

Mouse

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

THRB siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

53,916 Da

3D Structure

ModBase 3D Structure for P37242

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse THRB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for THRB sirna

RNA Interference (RNAi)

Other Notes

Small volumes of THRB sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for THRB. It may not necessarily be applicable to this product.

NCBI GI #

164663877

NCBI GeneID

21834

NCBI Accession #

NP_001106888.1

UniProt Primary Accession #

P37242

UniProt Secondary Accession #

P37244; Q0VDR8; Q3TY80

UniProt Related Accession #

P37242

GenBank Nucleotide #

NM_001113417.1

NCBI Official Full Name

thyroid hormone receptor beta isoform 1

NCBI Official Synonym Full Names

thyroid hormone receptor beta

NCBI Official Symbol

Thrb

NCBI Official Synonym Symbols

Nr1a2; Thrb1; Thrb2; T3R[b]; T3Rbeta; c-erbAbeta

UniProt Protein Name

Thyroid hormone receptor beta

UniProt Synonym Protein Names

Nuclear receptor subfamily 1 group A member 2; c-erbA-2; c-erbA-beta

Protein Family

Homoserine kinase

UniProt Gene Name

Thrb

UniProt Synonym Gene Names

Erba2; Nr1a2

UniProt Entry Name

THB_MOUSE

NCBI Protein Information

thyroid hormone receptor beta

UniProt Comments for THRB

TR-beta1: nuclear hormone receptor and transcription factor. High affinity receptor for triiodothyronine. Homolog of the viral oncogene erbB. Two splice variant isoforms have been described.

Protein type: Nuclear receptor; DNA-binding

Cellular Component: nuclear chromatin; nucleus

Molecular Function: protein binding; protein homodimerization activity; enzyme binding; DNA binding; zinc ion binding; chromatin DNA binding; sequence-specific DNA binding; metal ion binding; double-stranded DNA binding; steroid hormone receptor activity; thyroid hormone receptor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; regulation of lipid metabolic process; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; regulation of heart contraction; positive regulation of ossification; regulation of transcription from RNA polymerase II promoter; negative regulation of female receptivity; organ morphogenesis; sensory perception of sound; positive regulation of chondrocyte differentiation; female courtship behavior; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; negative regulation of transcription, DNA-dependent

Research Articles on THRB

1. Our findings indicated that synergistic signaling of KRAS(G12D) and TRbetaPV led to increased MYC expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with THRB sirna

Reference Product	PubMed Publications
Thra sirna	>56 publications with THRB and Thra
Rxra sirna	>2 publications with THRB and Rxra
Ncor1 sirna	>2 publications with THRB and Ncor1
Rxrg sirna	>1 publications with THRB and Rxrg

Pathways associated with THRB sirna

Products by Pathway	Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Generic Transcription Pathway antibodies	Generic Transcription Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Nuclear Receptor Transcription Pathway antibodies	Nuclear Receptor Transcription Pathway Diagram
Nuclear Receptors Pathway antibodies	Nuclear Receptors Pathway Diagram
Thyroid Hormone Signaling Pathway antibodies	Thyroid Hormone Signaling Pathway Diagram
XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway antibodies	XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway Diagram

Diseases associated with THRB sirna

Disease Name	Pubmed Publications
Thyroid Diseases Antibodies	>60 publications with THRB and Thyroid Diseases
Thyroid Hormone Resistance Syndrome Antibodies	>33 publications with THRB and Thyroid Hormone Resistance Syndrome
Nervous System Diseases Antibodies	>14 publications with THRB and Nervous System Diseases
Disease Models, Animal Antibodies	>10 publications with THRB and Disease Models, Animal
Cardiovascular Diseases Antibodies	>9 publications with THRB and Cardiovascular Diseases
Kidney Diseases Antibodies	>8 publications with THRB and Kidney Diseases
Hyperplasia Antibodies	>6 publications with THRB and Hyperplasia
Neoplasms, Experimental Antibodies	>5 publications with THRB and Neoplasms, Experimental
Brain Diseases Antibodies	>5 publications with THRB and Brain Diseases
Liver Diseases Antibodies	>3 publications with THRB and Liver Diseases

Organs/Tissues associated with THRB sirna

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>21 publications with THRB and Brain
Liver Antibodies	>18 publications with THRB and Liver
Kidney Antibodies	>12 publications with THRB and Kidney
Lung Antibodies	>12 publications with THRB and Lung
Pituitary Gland Antibodies	>11 publications with THRB and Pituitary Gland
Eye Antibodies	>9 publications with THRB and Eye
Inner Ear Antibodies	>8 publications with THRB and Inner Ear
Muscle Antibodies	>6 publications with THRB and Muscle
Embryonic Tissue Antibodies	>4 publications with THRB and Embryonic Tissue
Heart Antibodies	>3 publications with THRB and Heart