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USP2 siRNA – Ubiquitin Carboxyl-Terminal Hydrolase 2 Isoform Usp2-69 (Mouse)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8217614

MBS8217614 is an siRNA targeting USP2. Also known as USP2 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit USP2 expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
USP2, siRNA
Full Product Name
USP2 siRNA (Mouse)
Product Gene Name
USP2 sirna
Product Synonym Names
UBP41; Ubiquitin carboxyl-terminal hydrolase 2; 41 kDa ubiquitin-specific protease; Deubiquitinating enzyme 2; Ubiquitin thioesterase 2; Ubiquitin-specific-processing protease 2
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
USP2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
44,908 Da
3D Structure
ModBase 3D Structure for O88623
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse USP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for USP2 sirna
RNA Interference (RNAi)

Other Notes

Small volumes of USP2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for USP2. It may not necessarily be applicable to this product.

NCBI GI #
114052502
NCBI GeneID
53376
NCBI Accession #
NP_058088.2
UniProt Primary Accession #
O88623
UniProt Secondary Accession #
Q6X4T9; Q6X4U1; Q8VD74; Q9JJ87
UniProt Related Accession #
O88623
GenBank Nucleotide #
NM_016808.2
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 2 isoform Usp2-69
NCBI Official Synonym Full Names
ubiquitin specific peptidase 2
NCBI Official Symbol
Usp2
NCBI Official Synonym Symbols
Ubp41; B930035K21Rik
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 2
UniProt Synonym Protein Names
41 kDa ubiquitin-specific protease; Deubiquitinating enzyme 2; Ubiquitin thioesterase 2; Ubiquitin-specific-processing protease 2
Protein Family
Protein
UniProt Gene Name
Usp2
UniProt Synonym Gene Names
Ubp41
UniProt Entry Name
UBP2_MOUSE
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 2

UniProt Comments for USP2

USP2: Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Isoform 1 and isoform 4 possess both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells. Homooligomer. Found in trimeric complex with MDM2 and MDM4 and UPB2. Interacts with CCND1; the interaction is direct and promotes its stabilization by antagonizing ubiquitin-dependent degradation. Interacts (via N-terminus and C- terminus) with MDM2. Interacts with MDM4. Down-regulated by cisplatin. Expressed in mesangial cells of the kidney and in different types of glomerulonephritides. Cleavage is inhibited by ubiquitin in a dosage- dependent manner. Cleavage is blocked by ubiquitin aldehyde. Belongs to the peptidase C19 family. USP2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Protease; Ubiquitin-specific protease

Cellular Component: perinuclear region of cytoplasm; cytoplasm; cell cortex; nucleus

Molecular Function: peptidase activity; identical protein binding; hydrolase activity; metal ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; cysteine-type peptidase activity

Biological Process: ubiquitin-dependent protein catabolic process; locomotor rhythm; proteasomal ubiquitin-dependent protein catabolic process; muscle development; protein deubiquitination; regulation of transcription, DNA-dependent; rhythmic process; circadian regulation of gene expression; cell cycle; proteolysis; circadian behavior; entrainment of circadian clock by photoperiod
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Research Articles on USP2

1. A novel function of USP2 in the molecular clock in which it regulates PER1 function.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with USP2 sirna

 Reference Product  PubMed Publications
 Nedd4 sirna  >9 publications with USP2 and Nedd4
 Usp14 sirna  >3 publications with USP2 and Usp14
 Per1 sirna  >3 publications with USP2 and Per1
 Cry1 sirna  >2 publications with USP2 and Cry1
 Arntl sirna  >2 publications with USP2 and Arntl
 Usp13 sirna  >1 publications with USP2 and Usp13
 Uchl3 sirna  >1 publications with USP2 and Uchl3

Pathways associated with USP2 sirna

 Products by Pathway  Pathway Diagram
 TNF-alpha NF-kB Signaling Pathway antibodies  TNF-alpha NF-kB Signaling Pathway Diagram

Diseases associated with USP2 sirna

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >7 publications with USP2 and Inflammation
 Necrosis Antibodies  >7 publications with USP2 and Necrosis
 Fatty Liver Antibodies  >4 publications with USP2 and Fatty Liver
 Kidney Diseases Antibodies  >3 publications with USP2 and Kidney Diseases
 Breast Neoplasms Antibodies  >3 publications with USP2 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >3 publications with USP2 and Cardiovascular Diseases
 Liver Diseases Antibodies  >2 publications with USP2 and Liver Diseases
 Nervous System Diseases Antibodies  >2 publications with USP2 and Nervous System Diseases
 Atrophy Antibodies  >2 publications with USP2 and Atrophy
 Hypertension Antibodies  >2 publications with USP2 and Hypertension

Organs/Tissues associated with USP2 sirna

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >13 publications with USP2 and Liver
 Kidney Antibodies  >12 publications with USP2 and Kidney
 Muscle Antibodies  >11 publications with USP2 and Muscle
 Prostate Antibodies  >8 publications with USP2 and Prostate
 Blood Antibodies  >7 publications with USP2 and Blood
 Testis Antibodies  >6 publications with USP2 and Testis
 Brain Antibodies  >5 publications with USP2 and Brain
 Heart Antibodies  >4 publications with USP2 and Heart
 Embryonic Tissue Antibodies  >4 publications with USP2 and Embryonic Tissue
 Lung Antibodies  >2 publications with USP2 and Lung
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