WAPAL siRNA – Wings Apart-Like Protein Homolog Isoform 1 (Mouse)

Name: WAPAL siRNA; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS8231105
Price: 480 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8231105

MBS8231105 is an siRNA targeting WAPAL. Also known as WAPAL siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit WAPAL expression using RNA interference More details

$480 / 15 nmol +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

WAPAL, siRNA

Full Product Name

WAPAL siRNA (Mouse)

Product Gene Name

WAPAL sirna

Product Synonym Names

KIAA0261; WAPL; Wings apart-like protein homolog; Dioxin-inducible factor 2; DIF-2

Host

Synthetic

Species Reactivity

Mouse

Form/Format

Lyophilized powder

Purity/Purification

> 97%

Specificity

WAPAL siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.

Preparation and Storage

Shipped at 4 degree C. Store at -20 degree C for one year.

Molecular Weight

134,070 Da

3D Structure

ModBase 3D Structure for Q65Z40

Negative Control

siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).

Components

We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse WAPAL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.

Applications Tested/Suitable for WAPAL sirna

RNA Interference (RNAi)

Other Notes

Small volumes of WAPAL sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for WAPAL. It may not necessarily be applicable to this product.

NCBI GI #

100815493

NCBI GeneID

218914

NCBI Accession #

NP_001004436.2

UniProt Primary Accession #

Q65Z40

UniProt Secondary Accession #

Q6ZQE9; B9EIG8

UniProt Related Accession #

Q65Z40

GenBank Nucleotide #

NM_001004436.4

NCBI Official Full Name

wings apart-like protein homolog isoform 1

NCBI Official Synonym Full Names

WAPL cohesin release factor

NCBI Official Symbol

Wapl

NCBI Official Synonym Symbols

FOE; DIF-2; Wapal; BC037674; A530089A20Rik

UniProt Protein Name

Wings apart-like protein homolog

UniProt Synonym Protein Names

Dioxin-inducible factor 2; DIF-2

UniProt Gene Name

Wapal

UniProt Synonym Gene Names

Kiaa0261; Wapl; DIF-2

UniProt Entry Name

WAPL_MOUSE

NCBI Protein Information

wings apart-like protein homolog

NCBI Summary for WAPAL

Studies suggest that the protein encoded by this gene is important for the release of cohesin from chromatin. This gene product is thought to be essential for development, and reduced expression of this gene in cells causes defects in chromatin structure. High levels of expression of the human ortholog of this gene are observed in cervical cancers, and expression of the human ortholog of this gene in mice results in tumor formation. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Aug 2014]

UniProt Comments for WAPAL

WAPL: a chromatin-associated protein partner of Epstein-Barr nuclear protein 2. Associated with cervical carcinogenesis and tumor progression.

Protein type: DNA-binding

Cellular Component: cohesin complex; nucleoplasm; microtubule cytoskeleton; intracellular membrane-bound organelle; cytoplasm; synaptonemal complex; chromosome; nucleus; chromatin

Biological Process: mitosis; positive regulation of fibroblast proliferation; cell division; negative regulation of DNA replication; response to toxin; negative regulation of sister chromatid cohesion; regulation of chromosome segregation; cell cycle

Research Articles on WAPAL

1. High levels of WAPAL are observed in human cervical cancers, and expression of human WAPAL in mice causes tumor formation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with WAPAL sirna

Reference Product	PubMed Publications
Smc3 sirna	>21 publications with WAPAL and Smc3
Rad21 sirna	>9 publications with WAPAL and Rad21
Esco2 sirna	>4 publications with WAPAL and Esco2
Esco1 sirna	>4 publications with WAPAL and Esco1
Pds5b sirna	>2 publications with WAPAL and Pds5b
Pds5a sirna	>2 publications with WAPAL and Pds5a
Nipbl sirna	>1 publications with WAPAL and Nipbl
Stag1 sirna	>1 publications with WAPAL and Stag1

Pathways associated with WAPAL sirna

Products by Pathway	Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Cohesin Loading Onto Chromatin Pathway antibodies	Cohesin Loading Onto Chromatin Pathway Diagram
Establishment Of Sister Chromatid Cohesion Pathway antibodies	Establishment Of Sister Chromatid Cohesion Pathway Diagram
M Phase Pathway antibodies	M Phase Pathway Diagram
Mitotic Anaphase Pathway antibodies	Mitotic Anaphase Pathway Diagram
Mitotic Metaphase And Anaphase Pathway antibodies	Mitotic Metaphase And Anaphase Pathway Diagram
Mitotic Prometaphase Pathway antibodies	Mitotic Prometaphase Pathway Diagram
Mitotic Telophase/Cytokinesis Pathway antibodies	Mitotic Telophase/Cytokinesis Pathway Diagram
Resolution Of Sister Chromatid Cohesion Pathway antibodies	Resolution Of Sister Chromatid Cohesion Pathway Diagram

Diseases associated with WAPAL sirna

Disease Name	Pubmed Publications
Ovarian Neoplasms Antibodies	>1 publications with WAPAL and Ovarian Neoplasms
Congenital Abnormalities Antibodies	>1 publications with WAPAL and Congenital Abnormalities
Nervous System Diseases Antibodies	>1 publications with WAPAL and Nervous System Diseases
Cell Transformation, Neoplastic Antibodies	>1 publications with WAPAL and Cell Transformation, Neoplastic
Breast Neoplasms Antibodies	>1 publications with WAPAL and Breast Neoplasms

Organs/Tissues associated with WAPAL sirna

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1 publications with WAPAL and Blood
Testis Antibodies	>1 publications with WAPAL and Testis
Bone Marrow Antibodies	>1 publications with WAPAL and Bone Marrow
Eye Antibodies	>1 publications with WAPAL and Eye
Brain Antibodies	>1 publications with WAPAL and Brain
Embryonic Tissue Antibodies	>1 publications with WAPAL and Embryonic Tissue