• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us
Showing 1 to 50 of 99 found results for "198574"
Granulocyte-macrophage colony-stimulating factor (CSF2)

Recombinant Bovine Granulocyte-macrophage colony-stimulating factor (CSF2)

(#MBS957955)

Gene Names:
CSF2; CSF; GMCSF; GM-CSF
Other Names:
granulocyte-macrophage colony-stimulating factor; colony stimulating factor 2
Host/Reactivities:
Host: E Coli or Yeast or Baculovirus or Mammalian Cell
Purity:
Greater or equal to 85% purity as determined by SDS-PAGE.
  • Western Blot (WB)
  • Immunohistochemistry (IHC)
  • Immunohistochemistry (IHC)
  • Immunohistochemistry (IHC)

    Polyclonal Antibody to Interleukin 1 Beta (IL1b)

    (#MBS2026412)

    Gene Names:
    IL1b; IL1A
    Other Names:
    interleukin-1 alpha; interleukin 1 alpha
    Host/Reactivities:
    Host: Rabbit / Reactivity: Bos taurus; Bovine (Cattle)
    Purification:
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Applications:
    Western Blot (WB)
    Typical Testing Data/Standard Curve (for reference only)

    Bovine interleukin 4, IL-4 ELISA Kit

    (#MBS702621)

    Gene Names:
    IL4; IL-4; BSF-1
    Other Names:
    Bovine interleukin 4; IL-4 ELISA Kit; BCGF-1; BCGF1; BSF1; MGC79402; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; interleukin 4; interleukin-4; interleukin 4<
    Host/Reactivities:
    Reactivity: Bovine
    Assay Type
    Quantitative Sandwich
    Samples
    Serum, Plasma, Cell Culture Supernates, Tissue Homogenates
    Detection Range
    15.6 pg/ml-1000 pg/ml
    Sensitivity
    3.9 pg/ml.
    Typical Testing Data/Standard Curve (for reference only)

    Human G-CSF (Granulocyte Colony Stimulating Factor 3) ELISA Kit

    (#MBS2510091)

    Gene Names:
    G-CSF; CSF3
    Other Names:
    granulocyte colony-stimulating factor; colony stimulating factor 3 (granulocyte)<
    Host/Reactivities:
    Reactivity: Human
    Assay Type
    Sandwich
    Samples
    Serum, Plasma, Biological Fluids
    Detection Range
    39.063-2500pg/mL
    Sensitivity
    Min: 23.438pg/mL; Max: 2500pg/mL
    Typical Testing Data/Standard Curve (for reference only)

    Bovine Interferon beta, IFN-beta/IFNB ELISA Kit

    (#MBS704687)

    Gene Names:
    IFNB1; IFNb
    Other Names:
    Bovine Interferon beta; IFN-beta/IFNB ELISA Kit; IFB; IFF; MGC96956; interferon beta; interferon; beta 1; fibroblast; interferon beta-1
    Host/Reactivities:
    Reactivity: Bovine
    Assay Type
    Quantitative Competitive
    Samples
    Serum, plasma
    Detection Range
    8 pg/ml-200 pg/ml
    Sensitivity
    < 5 pg/ml
    • Western Blot (WB)
    • Immunohistochemistry (IHC)

      Polyclonal Antibody to Interleukin 15 (IL15)

      (#MBS2015644)

      Gene Names:
      IL15
      Other Names:
      interleukin-15; interleukin 15
      Host/Reactivities:
      Host: Rabbit / Reactivity: Bovine
      Purification:
      Antigen-specific Affinity Chromatography
      Applications:
      WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM
      Typical Testing Data/Standard Curve (for reference only)

      Rat G-CSF (Granulocyte Colony Stimulating Factor) ELISA Kit

      (#MBS2514139)

      Gene Names:
      G-CSF; CSF3
      Other Names:
      granulocyte colony-stimulating factor; colony stimulating factor 3 (granulocyte)<
      Host/Reactivities:
      Reactivity: Rat
      Assay Type
      Quantitative Sandwich
      Samples
      Serum, plasma and other biological fluids
      Detection Range
      15.63-1000pg/mL
      Sensitivity
      9.38pg/mL
      • Testing Data #1
      • Testing Data #2
      • Testing Data #3

        MOUSE ANTI BOVINE CD4

        (#MBS224612)

        Gene Names:
        CD4
        Other Names:
        T-cell surface glycoprotein CD4
        Host/Reactivities:
        Host: Mouse
        Isotype/Clone:
        Isotype: IgG2a / Clone CC8
        Applications:
        Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
        Typical Testing Data/Standard Curve (for reference only)

        Bovine Interleukin 2, IL-2 ELISA Kit

        (#MBS700611)

        Gene Names:
        IL2; IL-2; TCGF
        Other Names:
        Bovine Interleukin 2; IL-2 ELISA Kit; lymphokine; T cell growth factor; aldesleukin; interleukin-2; involved in regulation of T-cell clonal expansion; interleukin 2; interleukin 2<
        Host/Reactivities:
        Reactivity: Bovine
        Detection Range
        31.25 pg/ml-2000 pg/ml
        Sensitivity
        7.8 pg/ml
        Typical Testing Data/Standard Curve (for reference only)

        Mouse G-CSF (Granulocyte Colony Stimulating Factor) ELISA Kit

        (#MBS2511887)

        Gene Names:
        G-CSF; CSF3
        Other Names:
        granulocyte colony-stimulating factor; colony stimulating factor 3 (granulocyte)<
        Host/Reactivities:
        Reactivity: Mouse
        Assay Type
        Sandwich
        Samples
        Serum, Plasma, Biological Fluids
        Detection Range
        15.625-1000pg/mL
        Sensitivity
        Min: 9.375pg/mL; Max: 1000pg/mL
        IL-5

        IL-5

        (#MBS378239)

        Gene Names:
        IL-5; IL5
        Other Names:
        interleukin-5
        Host/Reactivities:
        Host: Rat / Reactivity: Mouse, Human
        Purification:
        >95%
        Isotype/Clone:
        Isotype: IgG1 / Clone TRFK5
        Applications:
        Blocking (BL)
        SDS-Page

        Recombinant Interferon Gamma (IFNg)

        (#MBS2010344)

        Gene Names:
        IFNg; IFNB1; IFNb
        Other Names:
        interferon beta-1; interferon; beta 1; fibroblast<
        Host/Reactivities:
        Host: E Coli
        Purity:
        > 95%
        Applications:
        SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
        IL-4

        RECOMBINANT BOVINE INTERLEUKIN-4

        (#MBS232227)

        Gene Names:
        IL4; IL-4; BSF-1
        Other Names:
        interleukin-4; interleukin 4<
        Purity:
        >95% by SDS PAGE analysis
        Applications:
        ELISA (EIA), Functional Assays (FN)
        Test Data

        Rat Anti-Mouse IL-5-UNLB

        (#MBS670070)

        Gene Names:
        IL5; TRF; IL-5
        Other Names:
        Rat Anti-Mouse IL-5 - Purified; Unlabeled Antibody; Rat Anti-Mouse IL-5; interleukin-5
        Host/Reactivities:
        Host: Rat / Reactivity: Mouse
        Isotype/Clone:
        Isotype: Rat IgG1 kappa / Clone TRFK5
        Applications:
        ELISA; Flow Cytometry; Immunofluorescence; Western Blot
        Interferon alpha-C (IFNAC)

        Recombinant Bovine Interferon alpha-C (IFNAC)

        (#MBS1066525)

        Gene Names:
        IFNAC
        Other Names:
        interferon alpha-C
        Host/Reactivities:
        Host: E Coli or Yeast or Baculovirus or Mammalian Cell
        Purity:
        Greater or equal to 85% purity as determined by SDS-PAGE.
        IL-5

        Rat Anti-Human IL-5-UNLB

        (#MBS670040)

        Gene Names:
        IL5; TRF; IL-5
        Other Names:
        Rat Anti-Human IL-5 - Purified; Unlabeled Antibody; Rat Anti-Human IL-5; interleukin-5
        Host/Reactivities:
        Host: Rat / Reactivity: Human
        Isotype/Clone:
        Isotype: Rat IgG2a kappa / Clone JES1-5A10
        Applications:
        ELISA
        IL-4

        MOUSE ANTI BOVINE INTERLEUKIN-4:FITC

        (#MBS224716)

        Gene Names:
        IL4; IL-4; BSF-1
        Other Names:
        interleukin-4; interleukin 4<
        Host/Reactivities:
        Host: Mouse
        Isotype/Clone:
        Isotype: IgG2a / Clone CC303
        Applications:
        Flow cytometry (FC/FACS)*
        Typical Testing Data/Standard Curve (for reference only)

        Human Interleukin 13, IL-13 ELISA Kit

        (#MBS703028)

        Gene Names:
        IL13
        Other Names:
        Human Interleukin 13; IL-13 ELISA KIT; ALRH; BHR1; IL-13; MGC116786; MGC116788; MGC116789; P600; ; interleukin 13; interleukin-13; interleukin 13<
        Host/Reactivities:
        Reactivity: Human
        Samples
        Serum, plasma and tissue homogenates
        Detection Range
        62.5 pg/ml-4000 pg/ml
        Sensitivity
        15.6 pg/ml
        IL-5

        Rat Anti-Mouse IL-5-UNLB

        (#MBS670072)

        Gene Names:
        IL5; TRF; IL-5
        Other Names:
        Rat Anti-Mouse IL-5 - Purified; Unlabeled Antibody; Rat Anti-Mouse IL-5; interleukin-5
        Host/Reactivities:
        Host: Rat / Reactivity: Mouse
        Isotype/Clone:
        Isotype: Rat IgG2a kappa / Clone TRFK4
        Applications:
        ELISA
        IL-5

        Rat Anti-Human IL-5-UNLB

        (#MBS670042)

        Gene Names:
        IL5; TRF; IL-5
        Other Names:
        Rat Anti-Human IL-5 - Purified; Unlabeled Antibody; Rat Anti-Human IL-5; interleukin-5
        Host/Reactivities:
        Host: Rat / Reactivity: Human
        Isotype/Clone:
        Isotype: Rat IgG2a kappa / Clone JES1-39D10
        Applications:
        ELISA; Flow Cytometry; Immunofluorescence; Western Blot
        IL-5

        Rat Anti-Human IL-5-BIOT

        (#MBS670041)

        Gene Names:
        IL5; TRF; IL-5
        Other Names:
        Rat Anti-Human IL-5 - Biotin; Rat Anti-Human IL-5; interleukin-5
        Host/Reactivities:
        Host: Rat / Reactivity: Human
        Isotype/Clone:
        Isotype: Rat IgG2a kappa / Clone JES1-5A10
        Applications:
        ELISA
        Typical Testing Data/Standard Curve (for reference only)

        Bovine Interferon beta-1 ELISA Kit

        (#MBS2885396)

        Gene Names:
        IFNB1; IFNb
        Other Names:
        Interferon beta-1
        Host/Reactivities:
        Reactivity: Bovine
        Samples
        Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
        Detection Range
        15.6-1000 pg/mL
        Interferon beta-2 (IFNB2)

        Recombinant Bovine Interferon beta-2 (IFNB2)

        (#MBS1262929)

        Gene Names:
        IFNB2; LOC517016
        Other Names:
        interferon beta-2
        Host/Reactivities:
        Host: E Coli or Yeast or Baculovirus or Mammalian Cell
        Purity:
        Greater or equal to 85% purity as determined by SDS-PAGE.
        IL-4

        MOUSE ANTI BOVINE INTERLEUKIN-4

        (#MBS215243)

        Gene Names:
        IL4; IL-4; BSF-1
        Other Names:
        interleukin-4; interleukin 4
        Host/Reactivities:
        Host: Mouse / Reactivity: Dog, Dolphin, Fin Whale, Goat, Horse, Mink, Mustelid, Pig, Sheep
        Isotype/Clone:
        Isotype: IgG2a / Clone CC303
        Applications:
        ELISA (EIA), Flow cytometry (FC/FACS)*
        • Testing Data
        • Testing Data

          CD4 (APC)

          (#MBS6268467)

          Gene Names:
          CD4
          Other Names:
          T-cell surface glycoprotein CD4
          Host/Reactivities:
          Host: Mouse / Reactivity: Bovine
          Purification:
          Purified by Protein A Affinity Chromatography
          Isotype/Clone:
          Isotype: IgG2a / Clone CC8
          Applications:
          Immunohistochemistry (IHC), Immunoprecipitation (IP)
          IL-4

          MOUSE ANTI BOVINE INTERLEUKIN-4:Biotin

          (#MBS210458)

          Gene Names:
          IL4; IL-4; BSF-1
          Other Names:
          interleukin-4; interleukin 4<
          Host/Reactivities:
          Host: Mouse
          Isotype/Clone:
          Isotype: IgG2b / Clone CC314
          Applications:
          ELISA (EIA), Flow cytometry (FC/FACS)*
          Typical Testing Data/Standard Curve (for reference only)

          Bovine Interleukin 13 (IL13) ELISA Kit

          (#MBS456390)

          Gene Names:
          IL13
          Other Names:
          interleukin-13
          Host/Reactivities:
          Reactivity: Bovine
          Samples
          bovine serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
          Detection Range
          31.2-2000pg/mL
          Sensitivity
          <13.4pg/mL
          IL-5

          Rat Anti-Mouse IL-5-BIOT

          (#MBS670073)

          Gene Names:
          IL5; TRF; IL-5
          Other Names:
          Rat Anti-Mouse IL-5 - Biotin; Rat Anti-Mouse IL-5; interleukin-5
          Host/Reactivities:
          Host: Rat / Reactivity: Mouse
          Isotype/Clone:
          Isotype: Rat IgG2a kappa / Clone TRFK4
          Applications:
          ELISA
          Typical Testing Data/Standard Curve (for reference only)

          Bovine Interleukin 13 (IL13) ELISA Kit

          (#MBS4500200)

          Gene Names:
          IL13
          Other Names:
          ALRH; BHR1; P600; IL-13; NC30; interleukin-13; interleukin 13
          Host/Reactivities:
          Reactivity: Bovine
          Assay Type
          Quantitative Sandwich
          Samples
          Human Serum, Plasma, Tissue Homogenates Or Other Biological Fluids
          Detection Range
          0.156-10ng/mL
          Sensitivity
          0.055ng/mL.
          Typical Testing Data/Standard Curve (for reference only)

          Bovine Granulocyte-macrophage colony-stimulating factor ELISA Kit

          (#MBS2882924)

          Gene Names:
          CSF2
          Other Names:
          Granulocyte-macrophage colony-stimulating factor; GM-CSF; Colony-stimulating factor; CSF
          Host/Reactivities:
          Reactivity: Bovine
          Samples
          Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
          Interleukin-4 (IL4)

          Recombinant Bovine Interleukin-4 (IL4)

          (#MBS948913)

          Gene Names:
          IL4; IL-4; BSF-1
          Other Names:
          interleukin-4; interleukin 4
          Host/Reactivities:
          Host: E Coli or Yeast or Baculovirus or Mammalian Cell
          Purity:
          Greater or equal to 85% purity as determined by SDS-PAGE.
          IL-5

          Rat Anti-Human IL-5-BIOT

          (#MBS670043)

          Gene Names:
          IL5; TRF; IL-5
          Other Names:
          Rat Anti-Human IL-5 - Biotin; Rat Anti-Human IL-5; interleukin-5
          Host/Reactivities:
          Host: Rat / Reactivity: Human
          Isotype/Clone:
          Isotype: Rat IgG2a kappa / Clone JES1-39D10
          Applications:
          ELISA; Flow Cytometry; Immunofluorescence; Western Blot
          • Testing Data
          • Testing Data

            MOUSE ANTI BOVINE CD4:FITC

            (#MBS215135)

            Gene Names:
            CD4
            Other Names:
            T-cell surface glycoprotein CD4
            Host/Reactivities:
            Host: Mouse
            Isotype/Clone:
            Isotype: IgG2a / Clone CC8
            Applications:
            Flow cytometry (FC/FACS)
            • Testing Data
            • Testing Data

              MOUSE ANTI BOVINE CD4:RPE

              (#MBS215137)

              Gene Names:
              CD4
              Other Names:
              T-cell surface glycoprotein CD4
              Host/Reactivities:
              Host: Mouse
              Isotype/Clone:
              Isotype: IgG2a / Clone CC8
              Applications:
              Flow cytometry (FC)
              Platelet-derived growth factor subunit A (PDGFA)

              Recombinant Bovine Platelet-derived growth factor subunit A (PDGFA)

              (#MBS1423381)

              Gene Names:
              PDGFA
              Other Names:
              platelet-derived growth factor subunit A; platelet derived growth factor subunit A
              Host/Reactivities:
              Host: E Coli or Yeast or Baculovirus or Mammalian Cell
              Purity:
              Greater or equal to 85% purity as determined by SDS-PAGE.
              Interleukin-5 (IL5)

              Recombinant Sheep Interleukin-5 (IL5)

              (#MBS1425454)

              Gene Names:
              IL5
              Other Names:
              interleukin-5
              Host/Reactivities:
              Host: E Coli or Yeast or Baculovirus or Mammalian Cell
              Purity:
              Greater or equal to 85% purity as determined by SDS-PAGE.
              Granulocyte colony-stimulating factor (CSF3)

              Recombinant Bovine Granulocyte colony-stimulating factor (CSF3)

              (#MBS953040)

              Gene Names:
              CSF3
              Other Names:
              granulocyte colony-stimulating factor; colony stimulating factor 3
              Host/Reactivities:
              Host: E Coli or Yeast or Baculovirus or Mammalian Cell
              Purity:
              Greater or equal to 85% purity as determined by SDS-PAGE.
              IL-5

              Rat Anti-Mouse IL-5-LE/AF

              (#MBS670071)

              Gene Names:
              IL5; TRF; IL-5
              Other Names:
              Rat Anti-Mouse IL-5 - Low Endotoxin/Azide-Free; Rat Anti-Mouse IL-5; interleukin-5
              Host/Reactivities:
              Host: Rat / Reactivity: Mouse
              Isotype/Clone:
              Isotype: Rat IgG1 kappa / Clone TRFK5
              Applications:
              ELISA; Flow Cytometry; Immunofluorescence; Western Blot
              Interferon alpha-A (IFNAA)

              Recombinant Bovine Interferon alpha-A (IFNAA)

              (#MBS1156822)

              Gene Names:
              IFNAA; IFN-a
              Other Names:
              interferon alpha-A
              Host/Reactivities:
              Host: E Coli or Yeast or Baculovirus or Mammalian Cell
              Purity:
              Greater or equal to 85% purity as determined by SDS-PAGE.
              • Sequence
              • 
Identification
              • 
SDS-Page

                Recombinant Interleukin 15 (IL15)

                (#MBS2018735)

                Gene Names:
                IL15
                Other Names:
                interleukin-15; interleukin 15<
                Host/Reactivities:
                Host: E.coli / Reactivity: Bos taurus; Bovine (Cattle)
                Purity:
                >90%
                Applications:
                SDS-PAGE, Western Blot (WB), Immunogen, Positive Control(May be suitable for use in other assays to be determined by the end user.)
                Western Blot (WB)

                Polyclonal Antibody to Colony Stimulating Factor 3, Granulocyte (GCSF)

                (#MBS2027763)

                Gene Names:
                GCSF; CSF3
                Other Names:
                granulocyte colony-stimulating factor; colony stimulating factor 3
                Host/Reactivities:
                Host: Rabbit / Reactivity: Bovine
                Purification:
                Affinity Chromatography
                Applications:
                Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
                • Testing Data
                • Testing Data

                  CD4 (Biotin)

                  (#MBS6248637)

                  Gene Names:
                  CD4
                  Other Names:
                  T-cell surface glycoprotein CD4
                  Host/Reactivities:
                  Host: Mouse / Reactivity: Bovine
                  Purification:
                  Purified by Protein A Affinity Chromatography
                  Isotype/Clone:
                  Isotype: IgG2a / Clone CC8
                  Applications:
                  Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
                  IL-4

                  MOUSE ANTI BOVINE INTERLEUKIN-4:RPE

                  (#MBS215244)

                  Gene Names:
                  IL4; IL-4; BSF-1
                  Other Names:
                  interleukin-4; interleukin 4<
                  Host/Reactivities:
                  Host: Mouse
                  Isotype/Clone:
                  Isotype: IgG2a / Clone CC303
                  Applications:
                  Flow cytometry (FC/FACS)*
                  Interferon beta-3 (IFNB3)

                  Recombinant Bovine Interferon beta-3 (IFNB3)

                  (#MBS1174187)

                  Gene Names:
                  IFNB3
                  Other Names:
                  interferon beta-3
                  Host/Reactivities:
                  Host: E Coli or Yeast or Baculovirus or Mammalian Cell
                  Purity:
                  Greater or equal to 85% purity as determined by SDS-PAGE.
                  Typical Testing Data/Standard Curve (for reference only)

                  Bovine Interleukin-13 ELISA Kit

                  (#MBS2887213)

                  Gene Names:
                  IL13
                  Other Names:
                  Interleukin-13; IL-13
                  Host/Reactivities:
                  Reactivity: Bovine
                  Typical Testing Data/Standard Curve (for reference only)

                  Bovine Interleukin 1 Beta (IL1b) Mini Samples ELISA Kit

                  (#MBS2087466)

                  Gene Names:
                  IL1b; IL1A
                  Other Names:
                  IL1-B; IL1-Beta; IL1F2; IL-1beta; Interleukin-1 Family Member 2; Catabolin; interleukin-1 alpha
                  Host/Reactivities:
                  Reactivity: Bovine
                  Assay Type
                  Double-antibody Sandwich
                  Samples
                  Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
                  Detection Range
                  15.6-1,000pg/mL
                  Sensitivity
                  5.9pg/mL
                  Interleukin-7 (IL7)

                  Recombinant Bovine Interleukin-7 (IL7)

                  (#MBS955773)

                  Gene Names:
                  IL7
                  Other Names:
                  Interleukin-7; interleukin 7
                  Host/Reactivities:
                  Host: E Coli or Yeast or Baculovirus or Mammalian Cell
                  Purity:
                  Greater or equal to 85% purity as determined by SDS-PAGE.
                  Typical Testing Data/Standard Curve (for reference only)

                  Bovine Interleukin-7 ELISA Kit

                  (#MBS2886906)

                  Gene Names:
                  IL7
                  Other Names:
                  Interleukin-7; IL-7
                  Host/Reactivities:
                  Reactivity: Bovine
                  Samples
                  Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
                  Detection Range
                  0.78-50 ng/mL
                  Sensitivity
                  < 0.38 ng/mL
                  Typical Testing Data/Standard Curve (for reference only)

                  Bovine Granulocyte colony-stimulating factor ELISA Kit

                  (#MBS2883535)

                  Gene Names:
                  CSF3
                  Other Names:
                  Granulocyte colony-stimulating factor; G-CSF; GCSF
                  Host/Reactivities:
                  Reactivity: Bovine
                  Interleukin-1 alpha (IL1A)

                  Recombinant Bovine Interleukin-1 alpha (IL1A)

                  (#MBS962710)

                  Gene Names:
                  IL1A
                  Other Names:
                  interleukin-1 alpha; interleukin 1 alpha
                  Host/Reactivities:
                  Host: E Coli or Yeast or Baculovirus or Mammalian Cell
                  Purity:
                  Greater or equal to 85% purity as determined by SDS-PAGE.

                  • Products by Diseases
                  • IgG Antibody
                  • Monoclonal Antibody
                  • Polyclonal Antibody
                  • Peptide
                  • Recombinant Protein
                  • Secondary Antibody
                  • MyBioSource
                  Request a Quote

                  Please fill out the form below and our representative will get back to you shortly.

                  MBS000000
                  Contact Us

                  Please fill out the form below and our representative will get back to you shortly.

                  MBS000000