Rat Anti-IL5 Monoclonal Antibody – Cat# MBS670073

Il-5 (IL5) Antibody – Rat Monoclonal

Name: IL5 Antibody; Monoclonal; Rat; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS670073
Price: 375 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS670073

MBS670073 is an antibody targeting IL5. Also known as Rat Anti-Mouse IL-5-BIOT. Reactive in Mouse. Host: Rat. More details

$375 / 0.5 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

IL-5 (IL5), Monoclonal Antibody

Full Product Name

Rat Anti-Mouse IL-5-BIOT

Product Gene Name

anti-IL5 antibody

Product Synonym Names

Rat Anti-Mouse IL-5 - Biotin; Rat Anti-Mouse IL-5

Clonality

Monoclonal

Isotype

Rat IgG2a kappa

Clone Number

TRFK4

Host

Rat

Species Reactivity

Mouse

Form/Format

Biotin (BIOT) Conjugate

Specificity

Mouse Interleukin-5 (IL-5)1-4 This is a neutralizing antibody.

Preparation and Storage

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Reagents are stable for the period shown on the label if stored as directed.

Molecular Weight

15,229 Da

Chromosome Location

Chromosome: 7; NC_007305.4 (20604695..20607095). Location: 7q15-q21

3D Structure

ModBase 3D Structure for P52173

Immunogen

Semi-purified mouse T cell clone supernatant.

Characterization

To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform to characteristics of a standard reference reagent.

Applications Tested/Suitable for anti-IL5 antibody

ELISA

Application Notes for anti-IL5 antibody

Research Application(s): ELISA Detection: TRFK4 monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying mouse IL-5 protein levels.1-4 Biotinylated TRFK4 antibody should be paired with purified TRFK5 antibody as the capture antibody, with purified recombinant mouse IL-5 as the standard.
Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.

Product References and Citations for anti-IL5 antibody

1. Schumacher, J.H., A. O'garra. B. Schrader, A. Van Kimmenade, M.W. Bond, T.R. Mosmann, and R.L. Coffman. 1988. The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 ELISA. J. Immunol. 141:1576-1581.
2. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.
3. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.
4. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and sera. J. Immunol. Meth. 166:201-214.

Other Notes

Small volumes of anti-IL5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IL5. It may not necessarily be applicable to this product.

NCBI GI #

27806873

NCBI GeneID

280825

NCBI Accession #

NP_776347.1

UniProt Primary Accession #

P52173

UniProt Secondary Accession #

A9QWQ8

UniProt Related Accession #

P52173

GenBank Nucleotide #

NM_173922.1

NCBI Official Full Name

interleukin-5

NCBI Official Symbol

IL5

NCBI Official Synonym Symbols

TRF; IL-5

UniProt Protein Name

Interleukin-5

UniProt Synonym Protein Names

Eosinophil differentiation factor; T-cell replacing factor

Protein Family

Interleukin

UniProt Gene Name

IL5

UniProt Entry Name

IL5_BOVIN

NCBI Protein Information

interleukin-5; T-cell replacing factor; eosinophil differentiation factor; colony-stimulating factor, eosinophil

UniProt Comments for IL5

Function: Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells

Subunit structure: Homodimer; disulfide-linked

Subcellular location: Secreted

Sequence similarities: Belongs to the IL-5 family.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-IL5 antibody

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Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokines And Inflammatory Response (BioCarta) Pathway antibodies	Cytokines And Inflammatory Response (BioCarta) Pathway Diagram

Diseases associated with anti-IL5 antibody

Disease Name	Pubmed Publications
Immune System Diseases Antibodies	>127 publications with IL5 and Immune System Diseases
Hypersensitivity Antibodies	>85 publications with IL5 and Hypersensitivity
Respiratory Tract Diseases Antibodies	>77 publications with IL5 and Respiratory Tract Diseases
Asthma Antibodies	>70 publications with IL5 and Asthma
Inflammation Antibodies	>60 publications with IL5 and Inflammation
Lung Diseases Antibodies	>57 publications with IL5 and Lung Diseases
Respiratory Hypersensitivity Antibodies	>56 publications with IL5 and Respiratory Hypersensitivity
Eosinophilia Antibodies	>49 publications with IL5 and Eosinophilia
Neoplasms Antibodies	>40 publications with IL5 and Neoplasms
Autoimmune Diseases Antibodies	>23 publications with IL5 and Autoimmune Diseases