Goat Anti-ABCB5 Polyclonal Antibody – Cat# MBS223834

ABCB5 Antibody – Goat Polyclonal

Name: ABCB5 Antibody; Polyclonal; Goat; MyBioSource
Brand: MyBioSource
SKU: MBS223834
Price: 710 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS223834

MBS223834 specifically recognises human ABCB5 (ATP-binding cassette sub-family B member 5), a P-glycoprotein and member of the conserved ABC transporter family, which acts as an energy-dependent efflux transporter for rhodamine. ABCB5 is primarily expressed… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

ABCB5, Polyclonal Antibody

Full Product Name

GOAT ANTI HUMAN ABCB5

Product Gene Name

anti-ABCB5 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Goat

Form/Format

Purified
Purified IgG - liquid

Concentration

IgG concentration 0.5mg/ml (lot specific)

Specificity

This item specifically recognises human ABCB5 (ATP-binding cassette sub-family B member 5), a P-glycoprotein and member of the conserved ABC transporter family, which acts as an energy-dependent efflux transporter for rhodamine.
ABCB5 is primarily expressed by skin progenitor cells and shown to act as a regulator of cell fusion and determinant of membrane potential. Studies have revealed the expression of ABCB5 in human malignant melanoma tumours, and in particular its association with CD133+ stem cell phenotype-expressing cells.

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Molecular Weight

89,832 Da

OMIM

611785

3D Structure

ModBase 3D Structure for Q2M3G0

Perservative Stabilisers

0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin

Immunogen

Peptide sequence C-QTQHRNTSKKAQ from the internal region of ABCB5 (NP_848654.3)
Buffer Solution||TRIS buffered saline

Antiserum Preparation

Antiserum to ABCB5 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Target Species

Human

Applications Tested/Suitable for anti-ABCB5 antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-ABCB5 antibody

Western Blot: This item detects a band of approximately 80kDa in A431cell lysates
ELISA: Maximum Dilution: 1/128000
Western Blotting: Minimum Dilution: 0.03; Maximum Dilution: 0.1ug/ml

Other Notes

Small volumes of anti-ABCB5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ABCB5 purchase

MBS223834 is an antibody targeting ABCB5. Also known as GOAT ANTI HUMAN ABCB5. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for ABCB5. It may not necessarily be applicable to this product.

NCBI GI #

255708477

NCBI GeneID

340273

NCBI Accession #

NP_001157413.1

UniProt Primary Accession #

Q2M3G0

UniProt Secondary Accession #

Q2M3I5; Q5I5Q7; Q5I5Q8; Q6KG50; A4D131; A7BKA4; B5MD19; B7WPL1; F8QQP8; F8QQP9; J3KQ04

UniProt Related Accession #

Q2M3G0

GenBank Nucleotide #

NM_001163941.1

NCBI Official Full Name

ATP-binding cassette sub-family B member 5 isoform 1

NCBI Official Synonym Full Names

ATP-binding cassette, sub-family B (MDR/TAP), member 5

NCBI Official Symbol

ABCB5

NCBI Official Synonym Symbols

ABCB5beta; EST422562; ABCB5alpha

UniProt Protein Name

ATP-binding cassette sub-family B member 5

UniProt Synonym Protein Names

ABCB5 P-gp; P-glycoprotein ABCB5

Protein Family

ABC transporter B family

UniProt Gene Name

ABCB5

UniProt Entry Name

ABCB5_HUMAN

NCBI Protein Information

ATP-binding cassette sub-family B member 5; ABCB5 P-gp; ATP-binding cassette protein; P-glycoprotein ABCB5

NCBI Summary for ABCB5

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008]

UniProt Comments for ABCB5

ABCB5: Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, ABC family; Transporter

Chromosomal Location of Human Ortholog: 7p21.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: efflux transmembrane transporter activity; ATPase activity, coupled to transmembrane movement of substances; ATP binding

Biological Process: regulation of membrane potential; metabolic process; compound eye corneal lens development; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; cell differentiation; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; transmembrane transport; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent

Research Articles on ABCB5

1. In melanoma-initiating cells, ABCB5 controls IL1beta secretion, which serves to maintain slow cycling, chemoresistant cells through an IL1beta/IL8/CXCR1 cytokine signaling circuit.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ABCB5 antibody

Products by Pathway	Pathway Diagram
ABC Transporters Pathway antibodies	ABC Transporters Pathway Diagram
ABC Transporters Pathway antibodies	ABC Transporters Pathway Diagram
ABC-family Proteins Mediated Transport Pathway antibodies	ABC-family Proteins Mediated Transport Pathway Diagram
Transmembrane Transport Of Small Molecules Pathway antibodies	Transmembrane Transport Of Small Molecules Pathway Diagram

Diseases associated with anti-ABCB5 antibody

Disease Name	Pubmed Publications
Melanoma Antibodies	>58 publications with ABCB5 and Melanoma
Skin Neoplasms Antibodies	>20 publications with ABCB5 and Skin Neoplasms
Leukemia Antibodies	>7 publications with ABCB5 and Leukemia
Leukemia, Myeloid, Acute Antibodies	>5 publications with ABCB5 and Leukemia, Myeloid, Acute
Cell Transformation, Neoplastic Antibodies	>5 publications with ABCB5 and Cell Transformation, Neoplastic
Disease Models, Animal Antibodies	>3 publications with ABCB5 and Disease Models, Animal
Neoplasms, Experimental Antibodies	>3 publications with ABCB5 and Neoplasms, Experimental
Lung Neoplasms Antibodies	>2 publications with ABCB5 and Lung Neoplasms
Necrosis Antibodies	>2 publications with ABCB5 and Necrosis
Inflammation Antibodies	>2 publications with ABCB5 and Inflammation

Organs/Tissues associated with anti-ABCB5 antibody

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>27 publications with ABCB5 and Skin
Testis Antibodies	>3 publications with ABCB5 and Testis
Intestine Antibodies	>2 publications with ABCB5 and Intestine
Brain Antibodies	>2 publications with ABCB5 and Brain
Kidney Antibodies	>1 publications with ABCB5 and Kidney