IFN ALPHA (IFNA3) Antibody – Rabbit Polyclonal

Polyclonal

IgG

Rabbit

Preservative Free
Purified IgG - liquid

IgG concentration 0.5 mg/ml (lot specific)

This item recognizes chicken Interferon alpha (chIFNalpha). The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken. A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent. This item cross reacts with IFN beta in western blots but does not show any cross reactivity with IFN gamma. In bioassays, this antiserum neutralizes chIFN-alpha, but not chIFN-beta, and can therefore be used to discriminate IFN alpha and IFN beta bioactivities, see Schwarz, H. et al. for details.

Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

22,116 Da

ModBase 3D Structure for P42165

Recombinant chicken IFN alpha (PAP004)
Buffer Solution||Phosphate buffered saline

Antisera to chicken Interferon alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography on protein G.

Chicken