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ADH1B Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6462231

MBS6462231 is an antibody targeting ADH1B. Also known as ADH1B, ID (Alcohol Dehydrogenase 1B, Alcohol Dehydrogenase Subunit beta, ADH2) (MaxLight 405). Reactive in Human. Host: Rabbit. More details

$1025 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ADH1B, Polyclonal Antibody
Full Product Name
ADH1B, ID (Alcohol Dehydrogenase 1B, Alcohol Dehydrogenase Subunit beta, ADH2) (MaxLight 405)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human ADH1B.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
35,412 Da
OMIM
103720
3D Structure
ModBase 3D Structure for P00325
Immunogen
Synthetic peptide selected from the center region of human ADH1B (KLH).
Conjugate
MaxLight405
Note
Preservative Free

Product Categories/Family for anti-ADH1B antibody

Applications Tested/Suitable for anti-ADH1B antibody
Western Blot (WB), FLISA
Application Notes for anti-ADH1B antibody
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ADH1B antibody

Western blot analysis of ADH1B Antibody (Center) in HepG2 cell line lysates (35ug/lane). ADH1B (arrow) was detected using the purified Pab.
Rabbit Anti-ADH1B Polyclonal Antibody – Cat# MBS6462231

Immunohistochemistry (IHC) of anti-ADH1B antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ADH1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-ADH1B Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6462231

Flow Cytometry (FC/FACS) of anti-ADH1B antibody

ADH1B Antibody (Center) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ADH1B Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6462231

Immunofluorescence (IF) of anti-ADH1B antibody

Confocal immunofluorescent analysis of ADH1B Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-ADH1B Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6462231

Product References and Citations for anti-ADH1B antibody

1. Alcohol intake, Am. J. Gastroenterol. 104(9), 2182-2188 (2009). 2. Nishimura, F.T., Nihon Arukoru Yakubutsu Igakkai Zasshi 44(3), 139-155 (2009).

Other Notes

Small volumes of anti-ADH1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ADH1B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
alcohol dehydrogenase 1B isoform 1
NCBI Official Synonym Full Names
alcohol dehydrogenase 1B (class I), beta polypeptide
NCBI Official Symbol
ADH1B
NCBI Official Synonym Symbols
ADH2; HEL-S-117
UniProt Protein Name
Alcohol dehydrogenase 1B
UniProt Synonym Protein Names
Alcohol dehydrogenase subunit beta
UniProt Gene Name
ADH1B
UniProt Synonym Gene Names
ADH2
UniProt Entry Name
ADH1B_HUMAN
NCBI Protein Information
alcohol dehydrogenase 1B

NCBI Summary for ADH1B

The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
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UniProt Comments for ADH1B

ADH1B: Dimer of identical or non-identical chains of three types; alpha, beta and gamma. Belongs to the zinc-containing alcohol dehydrogenase family.

Protein type: Lipid Metabolism - fatty acid; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.1.1.1; Oxidoreductase; Amino Acid Metabolism - tyrosine; Cofactor and Vitamin Metabolism - retinol; Xenobiotic Metabolism - drug metabolism - cytochrome P450

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: cytosol

Molecular Function: zinc ion binding; alcohol dehydrogenase activity, zinc-dependent

Biological Process: xenobiotic metabolic process; ethanol oxidation

Disease: Alcohol Dependence
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-ADH1B antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >5 publications with ADH1B and Liver
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