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AP1M1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6274031

The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the… More details

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Product Overview

Online Datasheet in PDF
Product Name
AP1M1, Polyclonal Antibody
Full Product Name
AP1M1, ID (AP1M1, CLTNM, AP-1 complex subunit mu-1, AP-mu chain family member mu1A, Adaptor protein complex AP-1 mu-1 subunit, Adaptor-related protein complex 1 mu-1 subunit, Clathrin assembly protein complex 1 medium chain 1, Clathrin coat assembly prote
Product Gene Name
anti-AP1M1 antibody
Product Synonym Names
AP1M1; CLTNM; AP-1 complex subunit mu-1; AP-mu chain family member mu1A; Adaptor protein complex AP-1 mu-1 subunit; Adaptor-related protein complex 1 mu-1 subunit; Clathrin assembly protein complex 1 medium chain 1; Clathrin coat assembly protein AP47; Clathrin coat-associated protein AP47; Golgi adaptor HA1/AP1 adaptin mu-1 subunit; Mu-adaptin 1; Mu1A-adaptin
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
49,840 Da
OMIM
603535
3D Structure
ModBase 3D Structure for Q9BXS5
Immunogen
AP1M1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-235 amino acids from the Central region of human AP1M1.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-AP1M1 antibody

Antibodies; Clathrin
Applications Tested/Suitable for anti-AP1M1 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-AP1M1 antibody
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-AP1M1 antibody

AP1M1 Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the AP1M1 antibody detected the AP1M1 protein (arrow).
Rabbit Anti-AP1M1 Polyclonal Antibody – Cat# MBS6274031

Immunohistochemistry (IHC) of anti-AP1M1 antibody

AP1M1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AP1M1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-AP1M1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6274031

Immunofluorescence (IF) of anti-AP1M1 antibody

Confocal immunofluorescent analysis of AP1M1 Antibody (Center) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-AP1M1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6274031

Product References and Citations for anti-AP1M1 antibody

Sawasdee, N., et al. Biochem. Biophys. Res. Commun. 401(1):85-91(2010). Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009). Noviello, C.M., et al. J. Virol. 82(3):1249-1258(2008). Medigeshi, G.R., et al. Traffic 9(1):121-132(2008). Roeth, J.F., et al. J. Cell Biol. 167(5):903-913(2004).

Other Notes

Small volumes of anti-AP1M1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AP1M1 purchase

MBS6274031 is an antibody targeting AP1M1. Also known as AP1M1, ID (AP1M1, CLTNM, AP-1 complex subunit mu-1, AP-mu chain family member mu1A, Adaptor protein complex AP-1 mu-1 subunit, Adaptor-related protein complex 1 mu-1 subunit, Clathrin assembly protein complex 1 medium chain 1, Clathrin coat assembly prote. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for AP1M1. It may not necessarily be applicable to this product.

NCBI GI #
14210504
NCBI GeneID
8907
NCBI Accession #
NP_115882.1
UniProt Primary Accession #
Q9BXS5
UniProt Secondary Accession #
Q4TTY5
UniProt Related Accession #
Q9BXS5
GenBank Nucleotide #
NM_032493.3
NCBI Official Full Name
AP-1 complex subunit mu-1 isoform 2
NCBI Official Synonym Full Names
adaptor-related protein complex 1, mu 1 subunit
NCBI Official Symbol
AP1M1
NCBI Official Synonym Symbols
AP47; CLTNM; MU-1A; CLAPM2
UniProt Protein Name
AP-1 complex subunit mu-1
UniProt Synonym Protein Names
AP-mu chain family member mu1A; Adaptor protein complex AP-1 subunit mu-1; Adaptor-related protein complex 1 subunit mu-1; Clathrin assembly protein complex 1 mu-1 medium chain 1; Clathrin coat assembly protein AP47; Clathrin coat-associated protein AP47; Golgi adaptor HA1/AP1 adaptin mu-1 subunit; Mu-adaptin 1; Mu1A-adaptin
Protein Family
AP-1 complex
UniProt Gene Name
AP1M1
UniProt Synonym Gene Names
CLTNM
UniProt Entry Name
AP1M1_HUMAN
NCBI Protein Information
AP-1 complex subunit mu-1; mu-adaptin 1; mu1A-adaptin; HA1 47 kDa subunit; clathrin adaptor protein AP47; AP-mu chain family member mu1A; golgi adaptor AP-1 47 kDa protein; clathrin coat assembly protein AP47; clathrin coat-associated protein AP47; adapto

NCBI Summary for AP1M1

The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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Research Articles on AP1M1

1. Adaptor protein 1 promotes cross-presentation through the same tyrosine signal in major histocompatibility complex class I as that targeted by HIV-1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AP1M1 antibody

 Reference Product  PubMed Publications
 AP1M2 antibody  >8 publications with AP1M1 and AP1M2

Pathways associated with anti-AP1M1 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Clathrin Derived Vesicle Budding Pathway antibodies  Clathrin Derived Vesicle Budding Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 E-cadherin Signaling In The Nascent Adherens Junction Pathway antibodies  E-cadherin Signaling In The Nascent Adherens Junction Pathway Diagram
 Golgi Associated Vesicle Biogenesis Pathway antibodies  Golgi Associated Vesicle Biogenesis Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 Host Interactions Of HIV Factors Pathway antibodies  Host Interactions Of HIV Factors Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram

Organs/Tissues associated with anti-AP1M1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with AP1M1 and Brain
 Blood Antibodies  >2 publications with AP1M1 and Blood
 Kidney Antibodies  >2 publications with AP1M1 and Kidney
 Ovary Antibodies  >1 publications with AP1M1 and Ovary
 Lung Antibodies  >1 publications with AP1M1 and Lung
 Uncharacterized Tissue Antibodies  >1 publications with AP1M1 and Uncharacterized Tissue
 Testis Antibodies  >1 publications with AP1M1 and Testis
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