Rabbit Anti-AP2S1 Polyclonal Antibody – Cat# MBS6274096

AP2S1 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the AP2S1 antibody detected the AP2S1 protein (arrow).

AP2S1 Antibody – Rabbit Polyclonal

Name: AP2S1 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6274096
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6274096

One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex.… More details

$1060 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

AP2S1, Polyclonal Antibody

Full Product Name

AP2S1, ID (AP2S1, AP17, CLAPS2, AP-2 complex subunit sigma, Adapter-related protein complex 2 sigma subunit, Adaptor protein complex AP-2 subunit sigma, Clathrin assembly protein 2 small chain, Clathrin coat assembly protein AP17, Clathrin coat-associated

Product Gene Name

anti-AP2S1 antibody

Product Synonym Names

AP2S1; AP17; CLAPS2; AP-2 complex subunit sigma; Adapter-related protein complex 2 sigma subunit; Adaptor protein complex AP-2 subunit sigma; Clathrin assembly protein 2 small chain; Clathrin coat assembly protein AP17; Clathrin coat-associated protein AP17; HA2 17kD subunit; Plasma membrane adaptor AP-2 17kD protein; Sigma2-adaptin

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

Purity/Purification

Purified by Protein A Affinity Chromatography.

Preparation and Storage

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

17,018 Da

Chromosome Location

Chromosome: 19; NC_000019.9 (47341423..47354203, complement). Location: 19q13.2-q13.3

OMIM

600740

3D Structure

ModBase 3D Structure for P53680

Immunogen

AP2S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-64 amino acids from the Central region of human AP2S1.

Conjugate

FITC

Note

Preservative Free

Special Handling

Light sensitive

Product Categories/Family for anti-AP2S1 antibody

Antibodies; Clathrin

Applications Tested/Suitable for anti-AP2S1 antibody

Western Blot (WB), FLISA

Application Notes for anti-AP2S1 antibody

WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-AP2S1 antibody

Stove, V., et al. J. Virol. 79(17):11422-11433(2005). Lu, W., et al. Neuron 47(3):407-421(2005). Collins, B.M., et al. Cell 109(4):523-535(2002). Yano, H., et al. J. Neurosci. 21 (3), RC125 (2001): Pearse, B.M., et al. Curr. Opin. Struct. Biol. 10(2):220-228(2000).

Other Notes

Small volumes of anti-AP2S1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AP2S1 purchase

MBS6274096 is an antibody targeting AP2S1. Also known as AP2S1, ID (AP2S1, AP17, CLAPS2, AP-2 complex subunit sigma, Adapter-related protein complex 2 sigma subunit, Adaptor protein complex AP-2 subunit sigma, Clathrin assembly protein 2 small chain, Clathrin coat assembly protein AP17, Clathrin coat-associated. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for AP2S1. It may not necessarily be applicable to this product.

NCBI GI #

70906430

NCBI GeneID

1175

NCBI Accession #

NP_004060.2

NCBI Related Accession #

NP_067586.1

UniProt Primary Accession #

P53680

UniProt Secondary Accession #

O75977; Q6PK67; B2R4Z4

UniProt Related Accession #

P53680

GenBank Nucleotide #

NM_004069.3

NCBI Official Full Name

AP-2 complex subunit sigma isoform AP17

NCBI Official Synonym Full Names

adaptor-related protein complex 2, sigma 1 subunit

NCBI Official Symbol

AP2S1

NCBI Official Synonym Symbols

AP17; HHC3; CLAPS2; AP17-DELTA

UniProt Protein Name

AP-2 complex subunit sigma

UniProt Synonym Protein Names

Adapter-related protein complex 2 subunit sigma; Adaptor protein complex AP-2 subunit sigma; Clathrin assembly protein 2 sigma small chain; Clathrin coat assembly protein AP17; Clathrin coat-associated protein AP17; HA2 17 kDa subunit; Plasma membrane adaptor AP-2 17 kDa protein; Sigma2-adaptin

Protein Family

AP-2 complex

UniProt Gene Name

AP2S1

UniProt Synonym Gene Names

AP17; CLAPS2

UniProt Entry Name

AP2S1_HUMAN

NCBI Protein Information

AP-2 complex subunit sigma; sigma2-adaptin; HA2 17 kDa subunit; clathrin coat assembly protein AP17; clathrin coat-associated protein AP17; clathrin assembly protein 2 small chain; adaptor protein complex AP-2 subunit sigma; plasma membrane adaptor AP-2 1

UniProt Comments for AP2S1

AP2S1: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. Belongs to the adaptor complexes small subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: plasma membrane; AP-2 adaptor complex; cytosol

Molecular Function: transporter activity; protein transporter activity

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; intracellular protein transport; axon guidance; synaptic transmission; clathrin cage assembly; viral reproduction; nerve growth factor receptor signaling pathway; regulation of endocytosis; ephrin receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; regulation of defense response to virus by virus

Disease: Hypocalciuric Hypercalcemia, Familial, Type Iii

Research Articles on AP2S1

1. Mutations in AP2S1 cause familial hypocalciuric hypercalcemia type 3.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AP2S1 antibody

Reference Product	PubMed Publications
AP1S1 antibody	>3 publications with AP2S1 and AP1S1

Pathways associated with anti-AP2S1 antibody

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
EGFR Downregulation Pathway antibodies	EGFR Downregulation Pathway Diagram
Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies	Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram
Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies	Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Glutamate Binding, Activation Of AMPA Receptors And Synaptic Plasticity Pathway antibodies	Glutamate Binding, Activation Of AMPA Receptors And Synaptic Plasticity Pathway Diagram

Diseases associated with anti-AP2S1 antibody

Disease Name	Pubmed Publications
Familial benign hypercalcemia, type 3 Antibodies	>1 publications with AP2S1 and Familial benign hypercalcemia, type 3

Organs/Tissues associated with anti-AP2S1 antibody

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>5 publications with AP2S1 and Nerve
Brain Antibodies	>3 publications with AP2S1 and Brain
Kidney Antibodies	>2 publications with AP2S1 and Kidney
Blood Antibodies	>1 publications with AP2S1 and Blood
Bone Antibodies	>1 publications with AP2S1 and Bone
Liver Antibodies	>1 publications with AP2S1 and Liver