Rabbit Anti-APIP Polyclonal Antibody – Cat# MBS9217228

All lanes : Anti-APIP Antibody (Center) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: Raji whole cell lysate
Lane 3: MCF-7 whole cell lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 27 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

APIP Antibody – Rabbit Polyclonal

Name: APIP Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS9217228
Price: 240 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9217228

Catalyzes the dehydration of methylthioribulose-1- phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1- phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and… More details

$240 / 0.05 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

APIP, Polyclonal Antibody

Full Product Name

APIP Antibody (Center)

Product Gene Name

anti-APIP antibody

Product Synonym Names

Methylthioribulose-1-phosphate dehydratase {ECO:0000255|HAMAP-Rule:MF_03116}; MTRu-1-P dehydratase {ECO:0000255|HAMAP-Rule:MF_03116}; 4.2.1.109 {ECO:0000255|HAMAP-Rule:MF_03116}; APAF1-interacting protein {ECO:0000255|HAMAP-Rule:MF_03116}; hAPIP; APIP {ECO:0000255|HAMAP-Rule:MF_03116}

Clonality

Polyclonal

Isotype

Host

Rabbit

Species Reactivity

Human

Form/Format

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Specificity

This APIP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 111-142 amino acids from the Central region of human APIP.

Preparation and Storage

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Antibody/Peptide Pairs

APIP peptide (MBS9230665) is used for blocking the activity of APIP antibody (MBS9217228)

Molecular Weight

27125 Da

OMIM

612491

3D Structure

ModBase 3D Structure for Q96GX9

Subtitle

Purified Rabbit Polyclonal Antibody (Pab)

Cellular Location

Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03116, ECO:0000269|PubMed:15262985, ECO:0000269|PubMed:23285211}.

Tissue Location

Isoform 1 is ubiquitously expressed. Isoform 2 is expressed at lower levels and detected in heart, brain, pancreas, liver, placenta, skeletal muscle and kidney.

Antigen Type

Synthesized Peptide

Product Categories/Family for anti-APIP antibody

Crown Antibodies; Primary Antibodies; Cell Biology

Applications Tested/Suitable for anti-APIP antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-APIP antibody

WB:~~1:2000

Product References and Citations for anti-APIP antibody

Lai C.-H.,et al.Genome Res. 10:703-713 (2000). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Taylor T.D.,et al.Nature 440:497-500 (2006). Cho D.-H.,et al.J. Biol. Chem. 279:39942-39950 (2004). Burkard T.R.,et al.BMC Syst. Biol. 5:17-17 (2011).

Other Notes

Small volumes of anti-APIP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for APIP purchase

MBS9217228 is an antibody targeting APIP. Also known as APIP Antibody (Center). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for APIP. It may not necessarily be applicable to this product.

NCBI GI #

166235186

NCBI GeneID

51074

NCBI Accession #

NP_057041.2

UniProt Primary Accession #

Q96GX9

UniProt Secondary Accession #

Q6PJX6; Q8WVU2; Q96HK2; Q9Y318; A8K9D3

UniProt Related Accession #

Q96GX9

GenBank Nucleotide #

NM_015957.3

NCBI Official Full Name

methylthioribulose-1-phosphate dehydratase

NCBI Official Synonym Full Names

APAF1 interacting protein

NCBI Official Symbol

APIP

NCBI Official Synonym Symbols

APIP2; CGI29; hAPIP; CGI-29; MMRP19

UniProt Protein Name

Methylthioribulose-1-phosphate dehydratase

UniProt Synonym Protein Names

APAF1-interacting protein

UniProt Gene Name

APIP

UniProt Synonym Gene Names

MTRu-1-P dehydratase; hAPIP

UniProt Entry Name

MTNB_HUMAN

NCBI Protein Information

methylthioribulose-1-phosphate dehydratase

NCBI Summary for APIP

APIP is an APAF1 (MIM 602233)-interacting protein that acts as a negative regulator of ischemic/hypoxic injury (Cho et al., 2004 [PubMed 15262985]).[supplied by OMIM, Dec 2008]

UniProt Comments for APIP

MMRP19: Catalyzes the dehydration of methylthioribulose-1- phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1- phosphate (DK-MTP-1-P). Has an anti-apoptotic function and prevents muscle ischemic damage. Inhibits the cytochrome c- dependent and APAF1-mediated cell death. Belongs to the aldolase class II family. MtnB subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - cysteine and methionine; Apoptosis; EC 4.2.1.109

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: cytoplasm

Molecular Function: 5-methylthioribulose-1-phosphate 4-dehydratase activity; identical protein binding; protein binding; zinc ion binding

Biological Process: apoptosis; methionine biosynthetic process from S-adenosylmethionine; methionine salvage; negative regulation of apoptosis

Research Articles on APIP

1. APIP/MtnB functions as a cell death inhibitor independently of its MtnB enzyme activity for apoptosis induced by either hypoxia or etoposide, but dependently for caspase-1-induced pyroptosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-APIP antibody

Reference Product	PubMed Publications
CASP9 antibody	>7 publications with APIP and CASP9
APAF1 antibody	>5 publications with APIP and APAF1

Pathways associated with anti-APIP antibody

Products by Pathway	Pathway Diagram
Cysteine And Methionine Metabolism Pathway antibodies	Cysteine And Methionine Metabolism Pathway Diagram
Cysteine And Methionine Metabolism Pathway antibodies	Cysteine And Methionine Metabolism Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Amino Acids And Derivatives Pathway antibodies	Metabolism Of Amino Acids And Derivatives Pathway Diagram
Metabolism Of Polyamines Pathway antibodies	Metabolism Of Polyamines Pathway Diagram
Methionine Salvage Pathway antibodies	Methionine Salvage Pathway Diagram
Methionine Salvage Pathway antibodies	Methionine Salvage Pathway Diagram
Methionine Salvage Pathway antibodies	Methionine Salvage Pathway Diagram
S-methyl-5-thio-alpha-D-ribose 1-phosphate Degradation Pathway antibodies	S-methyl-5-thio-alpha-D-ribose 1-phosphate Degradation Pathway Diagram

Diseases associated with anti-APIP antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>2 publications with APIP and Inflammation
Liver Diseases Antibodies	>1 publications with APIP and Liver Diseases
Fibrosis Antibodies	>1 publications with APIP and Fibrosis
Liver Neoplasms Antibodies	>1 publications with APIP and Liver Neoplasms

Organs/Tissues associated with anti-APIP antibody

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>4 publications with APIP and Lung
Bone Antibodies	>1 publications with APIP and Bone
Heart Antibodies	>1 publications with APIP and Heart
Thymus Antibodies	>1 publications with APIP and Thymus
Prostate Antibodies	>1 publications with APIP and Prostate
Liver Antibodies	>1 publications with APIP and Liver
Muscle Antibodies	>1 publications with APIP and Muscle
Kidney Antibodies	>1 publications with APIP and Kidney
Bone Marrow Antibodies	>1 publications with APIP and Bone Marrow
Brain Antibodies	>1 publications with APIP and Brain