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APOF Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6274499

The product of this gene is one of the minor apolipoproteins found in plasma. This protein forms complexes with lipoproteins and may be involved in transport and/or esterification of cholesterol. More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
APOF, Polyclonal Antibody
Full Product Name
APOF, ID (APOF, Apolipoprotein F, Lipid transfer inhibitor protein) (PE)
Product Gene Name
Product Synonym Names
APOF; Apolipoprotein F; Lipid transfer inhibitor protein
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
35,399 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (56754355..56756583, complement). Location: 12q13.3
OMIM
107760
3D Structure
ModBase 3D Structure for Q13790
Immunogen
APOF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-128 amino acids from the Central region of human APOF.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-APOF antibody

Applications Tested/Suitable for anti-APOF antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-APOF antibody
IHC: 1:50-100
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-APOF antibody

APOF Antibody (Center) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the APOF antibody detected the APOF protein (arrow).
Rabbit Anti-APOF Polyclonal Antibody – Cat# MBS6274499

Immunohistochemistry (IHC) of anti-APOF antibody

APOF antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the APOF antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-APOF Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6274499

Product References and Citations for anti-APOF antibody

Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010). Mosbruger, T.L., et al. J. Infect. Dis. 201(9):1371-1380(2010). Chen, S.N., et al. BMC Med. Genet. 10, 111 (2009): He, Y., et al. J. Lipid Res. 49(7):1529-1537(2008). Morton, R.E., et al. J. Lipid Res. 49(1):127-135(2008).

Other Notes

Small volumes of anti-APOF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for APOF purchase

MBS6274499 is an antibody targeting APOF. Also known as APOF, ID (APOF, Apolipoprotein F, Lipid transfer inhibitor protein) (PE). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for APOF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
apolipoprotein F
NCBI Official Synonym Full Names
apolipoprotein F
NCBI Official Symbol
APOF
NCBI Official Synonym Symbols
LTIP; Apo-F
UniProt Protein Name
Apolipoprotein F
UniProt Synonym Protein Names
Lipid transfer inhibitor protein
Protein Family
UniProt Gene Name
APOF
UniProt Synonym Gene Names
Apo-F; LTIP
UniProt Entry Name
APOF_HUMAN
NCBI Protein Information
apolipoprotein F; lipid transfer inhibitor protein

UniProt Comments for APOF

APOF: Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: extracellular region

Molecular Function: lipid transporter activity; cholesterol binding; receptor binding

Biological Process: cholesterol metabolic process; triacylglycerol metabolic process; cholesterol efflux; lipid metabolic process; lipid transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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