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BRSK1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6278980

BRSK1 may be involved as a checkpoint kinase in the regulation of G2/M arrest in response to UV-or methyl methane sulfonate (MMS)-induced, but not IR-induced, DNA damage. This protein phosphorylates WEE1 and CDC25B in vitro and CDC25C in vitro and in vivo.… More details

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Product Overview

Online Datasheet in PDF
Product Name
BRSK1, Polyclonal Antibody
Full Product Name
BRSK1, CT (BRSK1, KIAA1811, SAD1, SADB, Serine/threonine-protein kinase BRSK1, Brain-selective kinase 1, Brain-specific serine/threonine-protein kinase 1, Serine/threonine-protein kinase SAD-B, Synapses of Amphids Defective homolog 1) (Biotin)
Product Gene Name
anti-BRSK1 antibody
Product Synonym Names
BRSK1; KIAA1811; SAD1; SADB; Serine/threonine-protein kinase BRSK1; Brain-selective kinase 1; Brain-specific serine/threonine-protein kinase 1; Serine/threonine-protein kinase SAD-B; Synapses of Amphids Defective homolog 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
38,782 Da
OMIM
609235
3D Structure
ModBase 3D Structure for Q8TDC3
Immunogen
BRSK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human BRSK1.
Conjugate
Biotin
Note
Preservative Free

Product Categories/Family for anti-BRSK1 antibody

Antibodies; Protein Kinases
Applications Tested/Suitable for anti-BRSK1 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-BRSK1 antibody
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-BRSK1 antibody

KIAA1811 Antibody (S685) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the KIAA1811 antibody detected the KIAA1811 protein (arrow).
Rabbit Anti-BRSK1 Polyclonal Antibody – Cat# MBS6278980

Immunohistochemistry (IHC) of anti-BRSK1 antibody

BRSK1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BRSK1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-BRSK1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6278980

Product References and Citations for anti-BRSK1 antibody

Lu, R., et al., J. Biol. Chem. 279(30):31164-31170 (2004). Lizcano, J.M., et al., EMBO J. 23(4):833-843 (2004).

Other Notes

Small volumes of anti-BRSK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BRSK1 purchase

MBS6278980 is an antibody targeting BRSK1. Also known as BRSK1, CT (BRSK1, KIAA1811, SAD1, SADB, Serine/threonine-protein kinase BRSK1, Brain-selective kinase 1, Brain-specific serine/threonine-protein kinase 1, Serine/threonine-protein kinase SAD-B, Synapses of Amphids Defective homolog 1) (Biotin). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BRSK1. It may not necessarily be applicable to this product.

NCBI GI #
24308326
NCBI GeneID
84446
NCBI Accession #
NP_115806
UniProt Primary Accession #
Q8TDC3
UniProt Secondary Accession #
Q5J5B5; Q8NDD0; Q8NDR4; Q8TDC2; Q96AV4; Q96JL4; F1DG44
UniProt Related Accession #
Q8TDC3
GenBank Nucleotide #
NM_032430.1
NCBI Official Full Name
serine/threonine-protein kinase BRSK1
NCBI Official Synonym Full Names
BR serine/threonine kinase 1
NCBI Official Symbol
BRSK1
NCBI Official Synonym Symbols
hSAD1
UniProt Protein Name
Serine/threonine-protein kinase BRSK1
UniProt Synonym Protein Names
Brain-selective kinase 1 (EC:2.7.11.26); Brain-specific serine/threonine-protein kinase 1; BR serine/threonine-protein kinase 1; Serine/threonine-protein kinase SAD-B; Synapses of Amphids Defective homolog 1; SAD1 homolog; hSAD1
Protein Family
Serine/threonine-protein kinase
UniProt Gene Name
BRSK1
UniProt Synonym Gene Names
KIAA1811; SAD1; SADB; BR serine/threonine-protein kinase 1; SAD1 homolog; hSAD1
UniProt Entry Name
BRSK1_HUMAN
NCBI Protein Information
serine/threonine-protein kinase BRSK1

UniProt Comments for BRSK1

BRSK1: a serine/threonine protein kinase of the CAMK group. Closely related to AMPK. Activated by the kinase LKB1. Acts as checkpoint kinase upon DNA damage induced by UV or methyl methane sulfonate. Phosphorylates WEE1 and CDC25B in vitro and CDC25C in vitro and in vivo. Translocates from the cytoplasm to the nucleus in response to UV- or MMS-induced DNA damage. BRSK1 and BRSK2 are required for neuronal polarization. Widely expressed with highest levels in the brain and testis. Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.26; EC 2.7.11.1; Protein kinase, CAMK; Kinase, protein; CAMK group; CAMKL family; BRSK subfamily

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: nucleoplasm; centrosome; synaptic vesicle; cytoplasm; cell junction; nucleus

Molecular Function: protein serine/threonine kinase activity; gamma-tubulin binding; tau-protein kinase activity; magnesium ion binding; protein kinase binding; ATP binding

Biological Process: neuron differentiation; axonogenesis; establishment of cell polarity; neurotransmitter secretion; response to ionizing radiation; G2/M transition DNA damage checkpoint; centrosome duplication; response to DNA damage stimulus; protein amino acid phosphorylation; response to UV
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Research Articles on BRSK1

1. BRSK1 is a novel tumor suppressor in breast cancer which inversely correlated with Jab1 expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BRSK1 antibody

 Reference Product  PubMed Publications
 WEE1 antibody  >1 publications with BRSK1 and WEE1
 CDC25C antibody  >1 publications with BRSK1 and CDC25C

Pathways associated with anti-BRSK1 antibody

 Products by Pathway  Pathway Diagram
 LKB1 Signaling Events Pathway antibodies  LKB1 Signaling Events Pathway Diagram

Organs/Tissues associated with anti-BRSK1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >10 publications with BRSK1 and Brain
 Nerve Antibodies  >3 publications with BRSK1 and Nerve
 Testis Antibodies  >1 publications with BRSK1 and Testis
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