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CASP12 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6001433

Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CASP12, Polyclonal Antibody
Full Product Name
CASP12, ID (CASP12, Inactive caspase-12)
Product Gene Name
anti-Casp12 antibody
Product Synonym Names
Anti -CASP12, ID (CASP12, Inactive caspase-12)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Human
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
47,837 Da
Chromosome Location
Chromosome: 8; NC_005107.3 (2681125..2708341). Location: 8q11
3D Structure
ModBase 3D Structure for Q920D5
Immunogen
CASP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 172-201 amino acids from the Central region of human CASP12.

Product Categories/Family for anti-Casp12 antibody

Antibodies; Abs to Apoptosis; Caspase
Applications Tested/Suitable for anti-Casp12 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-Casp12 antibody
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50
Immunofluorescence: 1:10-50

Western Blot (WB) of anti-Casp12 antibody

Western Blot analysis in HL-60 cell line lysates (35ug/lane) using MBS6001433. This demonstrates that MBS6001433 detected the CASP12 protein (arrow).
Rabbit Anti-CASP12 Polyclonal Antibody – Cat# MBS6001433

Immunohistochemistry (IHC) of anti-Casp12 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue using MBS6001433 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6001433 for immunohistochemistry.
Rabbit Anti-CASP12 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6001433

Flow Cytometry (FC/FACS) of anti-Casp12 antibody

Flow Cytometric analysis of HL-60 cells using MBS6001433 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-CASP12 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6001433

Immunofluorescence (IF) of anti-Casp12 antibody

Confocal immunofluorescent analysis with 293 cells using MBS6001433 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-CASP12 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6001433

Other Notes

Small volumes of anti-Casp12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Casp12 purchase

MBS6001433 is an antibody targeting Casp12. Also known as CASP12, ID (CASP12, Inactive caspase-12). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Casp12. It may not necessarily be applicable to this product.

NCBI GI #
18426852
NCBI GeneID
156117
NCBI Accession #
NP_569106.1
UniProt Primary Accession #
Q920D5
UniProt Related Accession #
Q920D5
GenBank Nucleotide #
NM_130422.1
NCBI Official Full Name
caspase-12
NCBI Official Synonym Full Names
caspase 12
NCBI Official Symbol
Casp12
UniProt Protein Name
Caspase-12
Protein Family
Inactive caspase
UniProt Gene Name
Casp12
UniProt Synonym Gene Names
CASP-12
UniProt Entry Name
CASPC_RAT
NCBI Protein Information
caspase-12; CASP-12

NCBI Summary for Casp12

cysteine-aspartic acid protease (caspase); involved with the terminal stage of apoptosis [RGD, Feb 2006]

UniProt Comments for Casp12

CASP12: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.22.-

Cellular Component: intracellular membrane-bound organelle; endoplasmic reticulum; intracellular; nucleus; cytosol

Molecular Function: protein binding; protease binding; cysteine-type endopeptidase activity; endopeptidase activity; cysteine-type peptidase activity

Biological Process: response to drug; response to nicotine; response to inorganic substance; response to anoxia; positive regulation of apoptosis; apoptosis; response to iron(III) ion; unfolded protein response; positive regulation of caspase activity; response to organic nitrogen; proteolysis; regulation of apoptosis; protein autoprocessing; response to ethanol; regulation of inflammatory response; response to cold; aging
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Research Articles on Casp12

1. Activation of endoplasmic reticulum stress increases apoptosis and expression of CASP12 in the renal cortex of diabetic rats.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Casp12 antibody

 Reference Product  PubMed Publications
 Casp3 antibody  >54 publications with Casp12 and Casp3
 Ddit3 antibody  >32 publications with Casp12 and Ddit3
 Casp1 antibody  >10 publications with Casp12 and Casp1
 Tnf antibody  >8 publications with Casp12 and Tnf
 Apaf1 antibody  >5 publications with Casp12 and Apaf1
 Atf6 antibody  >5 publications with Casp12 and Atf6
 Capn1 antibody  >1 publications with Casp12 and Capn1
 Iapp antibody  >1 publications with Casp12 and Iapp
 Capn2 antibody  >1 publications with Casp12 and Capn2

Pathways associated with anti-Casp12 antibody

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 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Hepatitis B Pathway antibodies  Hepatitis B Pathway Diagram
 Prion Diseases Pathway antibodies  Prion Diseases Pathway Diagram
 Prion Diseases Pathway antibodies  Prion Diseases Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram

Diseases associated with anti-Casp12 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >32 publications with Casp12 and Nervous System Diseases
 Inflammation Antibodies  >23 publications with Casp12 and Inflammation
 Brain Diseases Antibodies  >22 publications with Casp12 and Brain Diseases
 Neoplasms Antibodies  >16 publications with Casp12 and Neoplasms
 Necrosis Antibodies  >12 publications with Casp12 and Necrosis
 Kidney Diseases Antibodies  >6 publications with Casp12 and Kidney Diseases
 Heart Diseases Antibodies  >6 publications with Casp12 and Heart Diseases
 Liver Diseases Antibodies  >6 publications with Casp12 and Liver Diseases
 Nerve Degeneration Antibodies  >5 publications with Casp12 and Nerve Degeneration
 Acute Kidney Injury Antibodies  >4 publications with Casp12 and Acute Kidney Injury

Organs/Tissues associated with anti-Casp12 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >13 publications with Casp12 and Liver
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