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CES2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6285250

CES2 is a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CES2, Polyclonal Antibody
Full Product Name
CES2, ID (CES2, ICE, Cocaine esterase, Carboxylesterase 2, Methylumbelliferyl-acetate deacetylase 2) (APC)
Product Gene Name
anti-CES2 antibody
Product Synonym Names
CES2; ICE; Cocaine esterase; Carboxylesterase 2; Methylumbelliferyl-acetate deacetylase 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human CES2.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
59,959 Da
OMIM
605278
3D Structure
ModBase 3D Structure for O00748
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 340-369 in the central region of human CES2, UniProt Accession #NP_003860; O00748.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-CES2 antibody

Antibodies; Enzymes
Applications Tested/Suitable for anti-CES2 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-CES2 antibody
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.

Testing Data of anti-CES2 antibody

All lanes: MBS647331 (1:2000) Lane 1: HepG2 whole cell lysates (20ug/lane) Lane 2: human liver lysates (20ug/lane). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000. Predicted band size: 62kD Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-CES2 Polyclonal Antibody – Cat# MBS6285250

Testing Data of anti-CES2 antibody

All lanes: MBS647331 (1:2000) Lane 1: SW620 whole cell lysates (20ug/lane) Lane 2: human liver lysates (20ug/lane). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000. Predicted band size: 62kD Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-CES2 Polyclonal Antibody, Testing Data – Cat# MBS6285250

Western Blot (WB) of anti-CES2 antibody

Western Blot analysis in HepG2 whole cell lysates (20ug/lane) using MBS647331 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 62kD Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-CES2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6285250

Immunohistochemistry (IHC) of anti-CES2 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma using MBS647331 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS647331 for immunohistochemistry.
Rabbit Anti-CES2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6285250

Flow Cytometry (FC/FACS) of anti-CES2 antibody

Flow Cytometric overlay histogram showing U-87 MG cells stained with MBS647331 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% BSA to block non-specific protein-protein interactions followed by033793 (1:25) for 60 min at 37 degree C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) at 1:400 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Rabbit Anti-CES2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6285250

Product References and Citations for anti-CES2 antibody

1. Holmes, R.S., et al. Mamm. Genome 21 (9-10), 427-441 (2010). 2. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 3. Howard, T.D., et al. Environ. Health Perspect. 118(10):1395-1399(2010). 4. Hatfield, M.J., et al. Br. J. Pharmacol. 160(8):1916-1928(2010). 5. Holmes, R.S., et al. Genetica 138(7):695-708(2010).

Other Notes

Small volumes of anti-CES2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CES2 purchase

MBS6285250 is an antibody targeting CES2. Also known as CES2, ID (CES2, ICE, Cocaine esterase, Carboxylesterase 2, Methylumbelliferyl-acetate deacetylase 2) (APC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CES2. It may not necessarily be applicable to this product.

NCBI GI #
37622885
NCBI GeneID
8824
NCBI Accession #
NP_003860
UniProt Primary Accession #
O00748
UniProt Secondary Accession #
Q16859; Q5MAB8; Q7Z366; Q8IUP4; Q8TCP8; A8K367
UniProt Related Accession #
O00748
GenBank Nucleotide #
NM_003869
NCBI Official Full Name
cocaine esterase isoform 1
NCBI Official Synonym Full Names
carboxylesterase 2
NCBI Official Symbol
CES2
NCBI Official Synonym Symbols
iCE; CE-2; PCE-2; CES2A1
UniProt Protein Name
Cocaine esterase
UniProt Synonym Protein Names
Carboxylesterase 2 (EC:3.1.1.1); CE-2; hCE-2; Methylumbelliferyl-acetate deacetylase 2 (EC:3.1.1.56)
Protein Family
Liver carboxylesterase
UniProt Gene Name
CES2
UniProt Synonym Gene Names
ICE; CE-2; hCE-2
UniProt Entry Name
EST2_HUMAN
NCBI Protein Information
cocaine esterase; carboxylesterase 2 (intestine, liver); hCE-2; intestinal carboxylesterase; liver carboxylesterase-2; methylumbelliferyl-acetate deacetylase 2

UniProt Comments for CES2

CES2: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.56; EC 3.1.1.84; Xenobiotic Metabolism - drug metabolism - other enzymes; Endoplasmic reticulum; EC 3.1.1.1; Hydrolase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: extracellular space; endoplasmic reticulum; endoplasmic reticulum lumen

Molecular Function: methylumbelliferyl-acetate deacetylase activity

Biological Process: catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CES2 antibody

 Products by Pathway  Pathway Diagram
 Drug Metabolism - Other Enzymes Pathway antibodies  Drug Metabolism - Other Enzymes Pathway Diagram
 Drug Metabolism - Other Enzymes Pathway antibodies  Drug Metabolism - Other Enzymes Pathway Diagram
 E2F Transcription Factor Network Pathway antibodies  E2F Transcription Factor Network Pathway Diagram
 Fluoropyrimidine Activity Pathway antibodies  Fluoropyrimidine Activity Pathway Diagram
 Irinotecan Pathway antibodies  Irinotecan Pathway Diagram
 Phase I, Non P450 Pathway antibodies  Phase I, Non P450 Pathway Diagram
 Retinol Biosynthesis Pathway antibodies  Retinol Biosynthesis Pathway Diagram

Diseases associated with anti-CES2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >25 publications with CES2 and Neoplasms
 Drug Toxicity Antibodies  >18 publications with CES2 and Drug Toxicity
 Liver Neoplasms Antibodies  >10 publications with CES2 and Liver Neoplasms
 Liver Diseases Antibodies  >8 publications with CES2 and Liver Diseases
 Inflammation Antibodies  >4 publications with CES2 and Inflammation
 Fatty Liver Antibodies  >4 publications with CES2 and Fatty Liver
 Lung Neoplasms Antibodies  >3 publications with CES2 and Lung Neoplasms
 Carcinoma, Hepatocellular Antibodies  >3 publications with CES2 and Carcinoma, Hepatocellular
 Hypertrophy Antibodies  >3 publications with CES2 and Hypertrophy
 Cardiovascular Diseases Antibodies  >2 publications with CES2 and Cardiovascular Diseases

Organs/Tissues associated with anti-CES2 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >65 publications with CES2 and Liver
 Intestine Antibodies  >29 publications with CES2 and Intestine
 Blood Antibodies  >19 publications with CES2 and Blood
 Kidney Antibodies  >10 publications with CES2 and Kidney
 Lung Antibodies  >9 publications with CES2 and Lung
 Brain Antibodies  >6 publications with CES2 and Brain
 Bone Antibodies  >4 publications with CES2 and Bone
 Heart Antibodies  >3 publications with CES2 and Heart
 Prostate Antibodies  >3 publications with CES2 and Prostate
 Muscle Antibodies  >3 publications with CES2 and Muscle
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