Rabbit Anti-DACT3 Polyclonal Antibody – Cat# MBS6290720

DACT3 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the DACT3 antibody detected the DACT3 protein (arrow).

DACT3 Antibody – Rabbit Polyclonal

Name: DACT3 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6290720
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6290720

Dact3 is the gene product of the third discovered member of the Dact (Dapper) family of developmentally-expressed genes. Discovered in mouse, a highly conserved ortholog also exists in the human genome. As with other Dact family members, Dact3 may play a role… More details

$1060 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DACT3, Polyclonal Antibody

Full Product Name

DACT3, NT (DACT3, RRR1, Dapper homolog 3, Antagonist of beta-catenin Dapper homolog 3, Arginine-rich region 1 protein) (Azide free) (HRP)

Product Gene Name

anti-DACT3 antibody

Product Synonym Names

DACT3; RRR1; Dapper homolog 3; Antagonist of beta-catenin Dapper homolog 3; Arginine-rich region 1 protein

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

Purity/Purification

Purified by Protein A Affinity Chromatography.

Preparation and Storage

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

64,949 Da

Chromosome Location

Chromosome: 19; NC_000019.9 (47150869..47164395, complement). Location: 19q13.32

OMIM

611112

3D Structure

ModBase 3D Structure for Q96B18

Immunogen

DACT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-165 amino acids from the N-terminal region of human DACT3.

Conjugate

HRP

Note

Preservative Free

Product Categories/Family for anti-DACT3 antibody

Antibodies; Wnt Proteins

Applications Tested/Suitable for anti-DACT3 antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-DACT3 antibody

WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-DACT3 antibody

Jiang, X., et al. Cancer Cell 13(6):529-541(2008). Fisher, D.A., et al. Dev. Dyn. 235(9):2620-2630(2006).

Other Notes

Small volumes of anti-DACT3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DACT3 purchase

MBS6290720 is an antibody targeting DACT3. Also known as DACT3, NT (DACT3, RRR1, Dapper homolog 3, Antagonist of beta-catenin Dapper homolog 3, Arginine-rich region 1 protein) (Azide free) (HRP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DACT3. It may not necessarily be applicable to this product.

NCBI GI #

182507165

NCBI GeneID

147906

NCBI Accession #

NP_659493.2

UniProt Primary Accession #

Q96B18

UniProt Related Accession #

Q96B18

GenBank Nucleotide #

NM_145056.2

NCBI Official Full Name

dapper homolog 3

NCBI Official Synonym Full Names

dishevelled-binding antagonist of beta-catenin 3

NCBI Official Symbol

DACT3

NCBI Official Synonym Symbols

RRR1; DAPPER3

UniProt Protein Name

Dapper homolog 3

UniProt Synonym Protein Names

Antagonist of beta-catenin Dapper homolog 3; Arginine-rich region 1 protein; Dapper antagonist of catenin 3

Protein Family

Dapper

UniProt Gene Name

DACT3

UniProt Synonym Gene Names

RRR1

UniProt Entry Name

DACT3_HUMAN

NCBI Protein Information

dapper homolog 3; arginine rich region 1; arginine-rich region 1 protein; dapper antagonist of catenin 3; antagonist of beta-catenin Dapper homolog 3; dapper, antagonist of beta-catenin, homolog 3

UniProt Comments for DACT3

DACT3: May be involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. Belongs to the dapper family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: cytoplasm

Molecular Function: identical protein binding; protein kinase C binding; protein kinase A binding

Biological Process: Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; negative regulation of cell growth

Research Articles on DACT3

1. Epigenetic repression of DACT3 leads to aberrant Wnt-beta-catenin signaling in colorectal cancer cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-DACT3 antibody

Disease Name	Pubmed Publications
Endocrine Gland Neoplasms Antibodies	>2 publications with DACT3 and Endocrine Gland Neoplasms
Ovarian Neoplasms Antibodies	>1 publications with DACT3 and Ovarian Neoplasms
Lung Diseases Antibodies	>1 publications with DACT3 and Lung Diseases
Fibrosis Antibodies	>1 publications with DACT3 and Fibrosis
Neoplasm Metastasis Antibodies	>1 publications with DACT3 and Neoplasm Metastasis

Organs/Tissues associated with anti-DACT3 antibody

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>5 publications with DACT3 and Embryonic Tissue
Kidney Antibodies	>2 publications with DACT3 and Kidney
Heart Antibodies	>1 publications with DACT3 and Heart
Lung Antibodies	>1 publications with DACT3 and Lung
Thymus Antibodies	>1 publications with DACT3 and Thymus
Brain Antibodies	>1 publications with DACT3 and Brain