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GASR Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9604307

Function: Receptor for gastrin and cholecystokinin. The CCK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins… More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GASR, Polyclonal Antibody
Full Product Name
GASR Antibody
Product Gene Name
anti-GASR antibody
Product Synonym Gene Name
CCKBR
Product Synonym Names
CCK B; CCK B receptor; CCK BR; CCK-B receptor; CCK-BR; CCK2 R; CCK2 receptor; CCK2-R; CCKB; CCKB receptor; CCKBR; CCKR Type B; CCKRB; Cholecystokinin 2 receptor; Cholecystokinin B receptor; Cholecystokinin-2 receptor; Cholecystokinin2 receptor; CholecystokininB receptor; GASR; GASR_HUMAN; Gastrin receptor; Gastrin/cholecystokinin type B receptor; Gastrincholecystokinin brain receptor
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
GASR antibody detects endogenous levels of total GASR
Antibody/Peptide Pairs
CCKBR peptide (MBS9623024) is used for blocking the activity of CCKBR antibody (MBS9604307)
Molecular Weight
Observed: 48 kDa
Predicted: 49 kDa
3D Structure
ModBase 3D Structure for P32239
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane
Tissue Specificity
Isoform 1 is expressed in brain, pancreas, stomach, the colon cancer cell line LoVo and the T-lymphoblastoma Jurkat, but not in heart, placenta, liver, lung, skeletal muscle, kidney or the stomach cancer cell line AGS. Expressed at high levels in the small cell lung cancer cell line NCI-H510, at lower levels in NCI-H345, NCI-H69 and GLC-28 cell lines, not expressed in GLC-19 cell line. Within the stomach, expressed at high levels in the mucosa of the gastric fundus and at low levels in the antrum and duodenum. Isoform 2 is present in pancreatic cancer cells and colorectal cancer cells, but not in normal pancreas or colonic mucosa. Isoform 3 is expressed in brain, pancreas, stomach, the stomach cancer cell line AGS and the colon cancer cell line LoVo.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (92%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-GASR antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-GASR antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-GASR antibody

MBS9604307 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-GASR Polyclonal Antibody – Cat# MBS9604307

Western Blot (WB) of anti-GASR antibody

Western blot analysis of GASR expression in A431 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-GASR Polyclonal Antibody, Western Blot (WB) – Cat# MBS9604307

Immunohistochemistry (IHC) of anti-GASR antibody

MBS9604307 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-GASR Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9604307

Other Notes

Small volumes of anti-GASR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GASR purchase

MBS9604307 is an antibody targeting GASR. Also known as GASR Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GASR. It may not necessarily be applicable to this product.

NCBI GI #
966751426
NCBI GeneID
887
NCBI Accession #
NP_001304958.1
UniProt Primary Accession #
P32239
UniProt Secondary Accession #
O75824; Q16144; Q92492; Q96LC6; Q9NYK7; Q9UBV1; A8K7P9
UniProt Related Accession #
P32239
GenBank Nucleotide #
NM_001318029.1
NCBI Official Full Name
gastrin/cholecystokinin type B receptor isoform 2
NCBI Official Synonym Full Names
cholecystokinin B receptor
NCBI Official Symbol
CCKBR
NCBI Official Synonym Symbols
GASR; CCK-B; CCK2R
UniProt Protein Name
Gastrin/cholecystokinin type B receptor
UniProt Synonym Protein Names
Cholecystokinin-2 receptor1 PublicationManual assertion based on opinion iniRef.19"Intermolecular interactions between cholecystokinin-8 and the third extracellular loop of the cholecystokinin-2 receptor."Giragossian C., Mierke D.F.Biochemistry 41:4560-4566(2002) [PubMed] [Europe PMC] [Abstract]Cited for: STRUCTURE BY NMR OF 352-379.
UniProt Gene Name
CCKBR
UniProt Synonym Gene Names
CCK-B receptor1 PublicationManual assertion based on opinion iniRef.4; CCK-BR; CCK2-R
NCBI Protein Information
gastrin/cholecystokinin type B receptor

NCBI Summary for GASR

This gene encodes a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. Alternative splicing results in multiple transcript variants. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors. [provided by RefSeq, Dec 2015]
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UniProt Comments for GASR

Receptor for gastrin and cholecystokinin. The CCK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
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Research Articles on GASR

1. High CCK2R expression is associated with cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GASR antibody

 Reference Product  PubMed Publications
 CCK antibody  >712 publications with GASR and CCK
 CCKAR antibody  >18 publications with GASR and CCKAR
 NTS antibody  >6 publications with GASR and NTS
 GRP antibody  >4 publications with GASR and GRP
 NPS antibody  >2 publications with GASR and NPS
 GCG antibody  >1 publications with GASR and GCG
 TAC1 antibody  >1 publications with GASR and TAC1
 GAST antibody  >1 publications with GASR and GAST

Pathways associated with anti-GASR antibody

 Products by Pathway  Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 GPCRs, Other Pathway antibodies  GPCRs, Other Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram

Diseases associated with anti-GASR antibody

 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >74 publications with GASR and Mental Disorders
 Nervous System Diseases Antibodies  >56 publications with GASR and Nervous System Diseases
 Disease Models, Animal Antibodies  >49 publications with GASR and Disease Models, Animal
 Panic Disorder Antibodies  >38 publications with GASR and Panic Disorder
 Liver Neoplasms Antibodies  >18 publications with GASR and Liver Neoplasms
 Inflammation Antibodies  >17 publications with GASR and Inflammation
 Hyperplasia Antibodies  >17 publications with GASR and Hyperplasia
 Hyperalgesia Antibodies  >15 publications with GASR and Hyperalgesia
 Atrophy Antibodies  >12 publications with GASR and Atrophy
 Necrosis Antibodies  >9 publications with GASR and Necrosis

Organs/Tissues associated with anti-GASR antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >263 publications with GASR and Brain
 Stomach Antibodies  >191 publications with GASR and Stomach
 Lung Antibodies  >25 publications with GASR and Lung
 Embryonic Tissue Antibodies  >7 publications with GASR and Embryonic Tissue
 Eye Antibodies  >4 publications with GASR and Eye
 Placenta Antibodies  >1 publications with GASR and Placenta
 Testis Antibodies  >1 publications with GASR and Testis
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