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HAND2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6305387

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular… More details

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Product Overview

Online Datasheet in PDF
Product Name
HAND2, Polyclonal Antibody
Full Product Name
HAND2, ID (HAND2, BHLHA26, DHAND, Heart-and neural crest derivatives-expressed protein 2, Class A basic helix-loop-helix protein 26, Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2) (Azide free) (HRP)
Product Gene Name
anti-HAND2 antibody
Product Synonym Names
HAND2; BHLHA26; DHAND; Heart-and neural crest derivatives-expressed protein 2; Class A basic helix-loop-helix protein 26; Deciduum; heart; autonomic nervous system and neural crest derivatives-expressed protein 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
20,269 Da
OMIM
602407
3D Structure
ModBase 3D Structure for P61296
Immunogen
HAND2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-110 amino acids from the Central region of human HAND2.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-HAND2 antibody

Antibodies; Transcription Factors
Applications Tested/Suitable for anti-HAND2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-HAND2 antibody
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Immunofluorescence (IF) of anti-HAND2 antibody

Fluorescent confocal image of Hela cell stained with HAND2 Antibody (Center).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HAND2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).HAND2 immunoreactivity is localized to Nucleus significantly.
Rabbit Anti-HAND2 Polyclonal Antibody – Cat# MBS6305387

Western Blot (WB) of anti-HAND2 antibody

HAND2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HAND2 antibody detected the HAND2 protein (arrow).
Rabbit Anti-HAND2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6305387

Product References and Citations for anti-HAND2 antibody

Shen, L., et al. Chin. Med. J. 123(13):1623-1627(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010): Voth, H., et al. BMC Mol. Biol. 10, 28 (2009): Morikawa, Y., et al. Circ. Res. 103(12):1422-1429(2008). Han, Z., et al. Development 133(6):1175-1182(2006).

Other Notes

Small volumes of anti-HAND2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HAND2 purchase

MBS6305387 is an antibody targeting HAND2. Also known as HAND2, ID (HAND2, BHLHA26, DHAND, Heart-and neural crest derivatives-expressed protein 2, Class A basic helix-loop-helix protein 26, Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2) (Azide free) (HRP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HAND2. It may not necessarily be applicable to this product.

NCBI GI #
88999597
NCBI GeneID
9464
NCBI Accession #
NP_068808.1
UniProt Primary Accession #
P61296
UniProt Secondary Accession #
O95300; O95301; P97833; Q494T1; B6ECG9
UniProt Related Accession #
P61296
GenBank Nucleotide #
NM_021973
NCBI Official Full Name
Homo sapiens heart and neural crest derivatives expressed 2 (HAND2), mRNA
NCBI Official Synonym Full Names
heart and neural crest derivatives expressed 2
NCBI Official Symbol
HAND2
NCBI Official Synonym Symbols
Hed; dHand; DHAND2; Thing2; bHLHa26
UniProt Protein Name
Heart- and neural crest derivatives-expressed protein 2
UniProt Synonym Protein Names
Class A basic helix-loop-helix protein 26; bHLHa26; Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2; dHAND
Protein Family
Heart- and neural crest derivatives-expressed protein
UniProt Gene Name
HAND2
UniProt Synonym Gene Names
BHLHA26; DHAND; bHLHa26; dHAND
UniProt Entry Name
HAND2_HUMAN
NCBI Protein Information
heart- and neural crest derivatives-expressed protein 2; basic helix-loop-helix transcription factor HAND2; class A basic helix-loop-helix protein 26; deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2

UniProt Comments for HAND2

HAND2: Essential for cardiac morphogenesis, particularly for the formation of the right ventricle and of the aortic arch arteries. Required for vascular development and regulation of angiogenesis, possibly through a VEGF signaling pathway. Plays also an important role in limb development, particularly in the establishment of anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud. Is involved in the development of branchial arches, which give rise to unique structures in the head and neck. Binds DNA on E-box consensus sequence 5'-CANNTG-3'.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 4q33

Cellular Component: transcription factor complex; protein complex; nuclear chromatin

Molecular Function: protein binding; protein homodimerization activity; transcription activator binding; AT DNA binding; protein heterodimerization activity; transcription coactivator activity; transcription factor binding

Biological Process: adult heart development; heart morphogenesis; tongue development; neural crest cell development; thymus development; transcription, DNA-dependent; in utero embryonic development; apoptosis; negative regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; heart development; mesenchymal cell proliferation; palate development; negative regulation of DNA binding; odontogenesis of dentine-containing teeth; peripheral nervous system neuron development; sympathetic nervous system development; regulation of transcription, DNA-dependent; suckling behavior; positive regulation of transcription from RNA polymerase II promoter; negative regulation of osteoblast differentiation; angiogenesis; heart looping; embryonic digit morphogenesis
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Research Articles on HAND2

1. suggest that HAND2 plays a key role in the regulation of progestin-induced decidualization of human endometrial stromal cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HAND2 antibody

 Reference Product  PubMed Publications
 SHH antibody  >27 publications with HAND2 and SHH
 GATA4 antibody  >27 publications with HAND2 and GATA4
 MEF2C antibody  >20 publications with HAND2 and MEF2C
 GLI3 antibody  >19 publications with HAND2 and GLI3
 EDN1 antibody  >16 publications with HAND2 and EDN1
 DLX6 antibody  >9 publications with HAND2 and DLX6
 MSX1 antibody  >7 publications with HAND2 and MSX1
 HOXD13 antibody  >7 publications with HAND2 and HOXD13
 TCF3 antibody  >5 publications with HAND2 and TCF3
 RUNX2 antibody  >2 publications with HAND2 and RUNX2

Pathways associated with anti-HAND2 antibody

 Products by Pathway  Pathway Diagram
 Heart Development Pathway antibodies  Heart Development Pathway Diagram

Diseases associated with anti-HAND2 antibody

 Disease Name  Pubmed Publications
 Heart Defects, Congenital Antibodies  >42 publications with HAND2 and Heart Defects, Congenital
 Carcinoma Antibodies  >6 publications with HAND2 and Carcinoma
 Nervous System Diseases Antibodies  >6 publications with HAND2 and Nervous System Diseases
 Tetralogy of Fallot Antibodies  >4 publications with HAND2 and Tetralogy of Fallot
 Adenocarcinoma Antibodies  >3 publications with HAND2 and Adenocarcinoma
 Fetal Death Antibodies  >3 publications with HAND2 and Fetal Death
 Fibrosis Antibodies  >3 publications with HAND2 and Fibrosis
 Neural Tube Defects Antibodies  >3 publications with HAND2 and Neural Tube Defects
 Neurobehavioral Manifestations Antibodies  >1 publications with HAND2 and Neurobehavioral Manifestations
 Abnormalities, Drug-Induced Antibodies  >1 publications with HAND2 and Abnormalities, Drug-Induced

Organs/Tissues associated with anti-HAND2 antibody

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >147 publications with HAND2 and Heart
 Embryonic Tissue Antibodies  >97 publications with HAND2 and Embryonic Tissue
 Bone Antibodies  >55 publications with HAND2 and Bone
 Nerve Antibodies  >30 publications with HAND2 and Nerve
 Brain Antibodies  >11 publications with HAND2 and Brain
 Uterus Antibodies  >7 publications with HAND2 and Uterus
 Placenta Antibodies  >7 publications with HAND2 and Placenta
 Adrenal Gland Antibodies  >5 publications with HAND2 and Adrenal Gland
 Intestine Antibodies  >5 publications with HAND2 and Intestine
 Mouth Antibodies  >4 publications with HAND2 and Mouth
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