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KI67 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4750037

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly(PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the… More details

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Product Overview

Product Name
Ki67, Polyclonal Antibody
Full Product Name
Anti-Ki67 Rabbit pAb
Product Gene Name
anti-Ki67 antibody
Product Synonym Names
MKI67; KIA; Antigen KI-67; Ki-67
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity Chromatography
Specificity
Ki67 Antibody detects endogenous levels of total Ki67
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Molecular Weight
359kDa
OMIM
176741
3D Structure
ModBase 3D Structure for P46013
Immunogen
A synthesized peptide derived from human Ki67
Subcellular Location
Cell junction; Tight junction
Applications Tested/Suitable for anti-Ki67 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-Ki67 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50

Western Blot (WB) of anti-Ki67 antibody

Western blot analysis of Ki67 expression in Ramos cell lysate.
Rabbit Anti-KI67 Polyclonal Antibody – Cat# MBS4750037

Immunohistochemistry (IHC) of anti-Ki67 antibody

Immunohistochemical analysis of paraffin-embedded human tonsil, using Ki67 Antibody.
Rabbit Anti-KI67 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4750037

Immunofluorescence (IF) of anti-Ki67 antibody

Immunofluorescent analysis of Hela cells, using Ki67 Antibody.
Rabbit Anti-KI67 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS4750037

Other Notes

Small volumes of anti-Ki67 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ki67 purchase

MBS4750037 is an antibody targeting Ki67. Also known as Anti-Ki67 Rabbit pAb. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Ki67. It may not necessarily be applicable to this product.

NCBI GI #
225543215
NCBI GeneID
4288
NCBI Accession #
NP_001139438.1
UniProt Primary Accession #
P46013
UniProt Secondary Accession #
Q5VWH2
UniProt Related Accession #
P46013
GenBank Nucleotide #
NM_001145966.1
NCBI Official Full Name
proliferation marker protein Ki-67 isoform 2
NCBI Official Synonym Full Names
marker of proliferation Ki-67
NCBI Official Symbol
MKI67
NCBI Official Synonym Symbols
KIA; MIB-; MIB-1; PPP1R105
UniProt Protein Name
Proliferation marker protein Ki-67
UniProt Synonym Protein Names
Antigen identified by monoclonal antibody Ki-67
Protein Family
Proliferation marker protein
UniProt Gene Name
MKI67
UniProt Synonym Gene Names
Antigen KI-67Curated; Antigen Ki67Curated
NCBI Protein Information
proliferation marker protein Ki-67

NCBI Summary for Ki67

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

UniProt Comments for Ki67

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
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Research Articles on Ki67

1. The different values of the cycling nuclear area major dimension may also be connected with the biological behaviour of the three examined groups. Moreover, the endometrial epithelial cells may follow a Ki-67 increase pathway, instead of the relatively stable pathway which the rapidly proliferating adenocarcinoma cells may use.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Ki67 antibody

 Reference Product  PubMed Publications
 MKI67IP antibody  >8 publications with Ki67 and MKI67IP
 CBX3 antibody  >1 publications with Ki67 and CBX3

Diseases associated with anti-Ki67 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >9254 publications with Ki67 and Neoplasms
 Adenocarcinoma Antibodies  >2117 publications with Ki67 and Adenocarcinoma
 Breast Neoplasms Antibodies  >1548 publications with Ki67 and Breast Neoplasms
 Nervous System Diseases Antibodies  >1433 publications with Ki67 and Nervous System Diseases
 Neoplasm Metastasis Antibodies  >1044 publications with Ki67 and Neoplasm Metastasis
 Carcinoma, Squamous Cell Antibodies  >981 publications with Ki67 and Carcinoma, Squamous Cell
 Adenoma Antibodies  >693 publications with Ki67 and Adenoma
 Hyperplasia Antibodies  >583 publications with Ki67 and Hyperplasia
 Colorectal Neoplasms Antibodies  >581 publications with Ki67 and Colorectal Neoplasms
 Neoplasms, Experimental Antibodies  >534 publications with Ki67 and Neoplasms, Experimental

Organs/Tissues associated with anti-Ki67 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1742 publications with Ki67 and Blood
 Brain Antibodies  >1239 publications with Ki67 and Brain
 Vascular Antibodies  >1110 publications with Ki67 and Vascular
 Lymph Node Antibodies  >1097 publications with Ki67 and Lymph Node
 Lymph Antibodies  >1092 publications with Ki67 and Lymph
 Skin Antibodies  >869 publications with Ki67 and Skin
 Lung Antibodies  >635 publications with Ki67 and Lung
 Liver Antibodies  >604 publications with Ki67 and Liver
 Muscle Antibodies  >596 publications with Ki67 and Muscle
 Bone Antibodies  >523 publications with Ki67 and Bone
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