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LAPTM5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6315180

MBS6315180 is an antibody targeting LAPTM5. Also known as LAPTM5, NT (LAPTM5, KIAA0085, Lysosomal-associated transmembrane protein 5, Lysosomal-associated multitransmembrane protein 5, Retinoic acid-inducible E3 protein) (MaxLight 550). Reactive in Human.… More details

$1060 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LAPTM5, Polyclonal Antibody
Full Product Name
LAPTM5, NT (LAPTM5, KIAA0085, Lysosomal-associated transmembrane protein 5, Lysosomal-associated multitransmembrane protein 5, Retinoic acid-inducible E3 protein) (MaxLight 550)
Product Gene Name
Product Synonym Names
LAPTM5; KIAA0085; Lysosomal-associated transmembrane protein 5; Lysosomal-associated multitransmembrane protein 5; Retinoic acid-inducible E3 protein
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Specificity
Recognizes human LAPTM5.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
29,937 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (31205315..31230683, complement). Location: 1p34
OMIM
601476
3D Structure
ModBase 3D Structure for Q13571
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 23-49 in the N-terminal region of human LAPTM5, UniProt Accession #Q13571. Species sequence homology: bovine
Conjugate
MaxLight550
Note
Preservative Free
Applications Tested/Suitable for anti-LAPTM5 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
Application Notes for anti-LAPTM5 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-LAPTM5 antibody

Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS646069. This demonstrates that MBS646069 detected the LAPTM5 protein (arrow).
Rabbit Anti-LAPTM5 Polyclonal Antibody – Cat# MBS6315180

Immunohistochemistry (IHC) of anti-LAPTM5 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph node using MBS646069 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS646069 for immunohistochemistry.
Rabbit Anti-LAPTM5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6315180

Flow Cytometry (FC/FACS) of anti-LAPTM5 antibody

Flow Cytometry analysis of NCI-H460 cells using MBS646069 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-LAPTM5 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6315180

Other Notes

Small volumes of anti-LAPTM5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LAPTM5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
lysosomal-associated transmembrane protein 5
NCBI Official Synonym Full Names
lysosomal protein transmembrane 5
NCBI Official Symbol
LAPTM5
NCBI Official Synonym Symbols
CLAST6
UniProt Protein Name
Lysosomal-associated transmembrane protein 5
UniProt Synonym Protein Names
Lysosomal-associated multitransmembrane protein 5; Retinoic acid-inducible E3 protein
UniProt Gene Name
LAPTM5
UniProt Synonym Gene Names
KIAA0085
UniProt Entry Name
LAPM5_HUMAN
NCBI Protein Information
lysosomal-associated transmembrane protein 5; retinoic acid-inducible E3 protein; human retinoic acid-inducible E3 protein; CD40-ligand-activated specific transcripts; lysosomal multispanning membrane protein 5; lysosomal-associated multitransmembrane pro

NCBI Summary for LAPTM5

This gene encodes a transmembrane receptor that is associated with lysosomes. The encoded protein, also known as E3 protein, may play a role in hematopoiesis. [provided by RefSeq, Feb 2009]

UniProt Comments for LAPTM5

Function: May have a special functional role during embryogenesis and in adult hematopoietic cells. Ref.1

Subunit structure: Binds to ubiquitin. Ref.1

Subcellular location: Lysosome membrane; Multi-pass membrane protein Ref.1.

Tissue specificity: Preferentially expressed in adult hematopoietic tissues. High levels in lymphoid and myeloid tissues. Highly expressed in peripheral blood leukocytes, thymus, spleen and lung, followed by placenta, liver and kidney. Ref.1

Induction: By retinoic acid.

Sequence similarities: Belongs to the LAPTM4/LAPTM5 transporter family.

Sequence caution: The sequence BAA07643.1 differs from that shown. Reason: Erroneous initiation.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-LAPTM5 antibody

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