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MBD5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8103148

MBD5 (Methyl-CpG-binding domain 5) is a critical gene for normal development. Microdeletions of 2q23.1 disrupting MBD5 and loss of function mutations of MBD5 cause MBD5-Associated Neurodevelopmental disorders (MAND).MBD5-associated neurodevelopmental disorder… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MBD5, Polyclonal Antibody
Full Product Name
Anti-MBD5 Antibody, Rabbit Polyclonal
Product Gene Name
anti-MBD5 antibody
Product Synonym Names
MBD5 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-MRD1 Antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid; 0.2um filtered solution in PBS
Purity/Purification
Protein A & Antigen Affinity
Specificity
Human MBD5
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Molecular Weight
1494
OMIM
phenotype 611472
3D Structure
ModBase 3D Structure for Q9P267
Immunogen
A synthetic peptide corresponding to the center region of the Human MBD5
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human MBD5, and purified by antigen affinity chromatography.
Applications Tested/Suitable for anti-MBD5 antibody
Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-MBD5 antibody
IHC-P: 1:100-1:500
ICC/IF: 1:300-1:10,000

Immunohistochemistry (IHC) of anti-MBD5 antibody

Immunochemical staining of human MBD5 in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.
Rabbit Anti-MBD5 Polyclonal Antibody – Cat# MBS8103148

Immunohistochemistry (IHC) of anti-MBD5 antibody

Immunochemical staining of human MBD5 in human placenta with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.
Rabbit Anti-MBD5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS8103148

Immunofluorescence (IF) of anti-MBD5 antibody

Immunofluorescence staining of MBD5 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MBD5 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
Rabbit Anti-MBD5 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS8103148

Other Notes

Small volumes of anti-MBD5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MBD5 purchase

MBS8103148 is an antibody targeting MBD5. Also known as Anti-MBD5 Antibody, Rabbit Polyclonal. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MBD5. It may not necessarily be applicable to this product.

NCBI GI #
296439306
NCBI GeneID
55777
NCBI Accession #
Q9P267.3
UniProt Primary Accession #
Q9P267
UniProt Secondary Accession #
Q53SR1; Q9NUV6; A5HMQ4; A7E2B1
UniProt Related Accession #
Q9P267
NCBI Official Full Name
Methyl-CpG-binding domain protein 5
NCBI Official Synonym Full Names
methyl-CpG binding domain protein 5
NCBI Official Symbol
MBD5
NCBI Official Synonym Symbols
MRD1
UniProt Protein Name
Methyl-CpG-binding domain protein 5
UniProt Synonym Protein Names
Methyl-CpG-binding protein MBD5
Protein Family
Methyl-CpG-binding domain-containing protein
UniProt Gene Name
MBD5
UniProt Synonym Gene Names
KIAA1461
UniProt Entry Name
MBD5_HUMAN
NCBI Protein Information
methyl-CpG-binding domain protein 5; methyl-CpG-binding protein MBD5

NCBI Summary for MBD5

This gene encodes a member of the methyl-CpG-binding domain (MBD) family. The MBD consists of about 70 residues and is the minimal region required for a methyl-CpG-binding protein binding specifically to methylated DNA. In addition to the MBD domain, this protein contains a PWWP domain (Pro-Trp-Trp-Pro motif), which consists of 100-150 amino acids and is found in numerous proteins that are involved in cell division, growth and differentiation. Mutations in this gene cause mental retardation autosomal dominant type 1. Haploinsufficiency of this gene is associated with a syndrome involving microcephaly, intellectual disabilities, severe speech impairment, and seizures. Alternatively spliced transcript variants have been found, but their full-length nature is not determined. [provided by RefSeq, Mar 2010]
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UniProt Comments for MBD5

MBD5: Binds to heterochromatin. Does not interact with either methylated or unmethylated DNA (in vitro). Defects in MBD5 are the cause of mental retardation autosomal dominant type 1 (MRD1). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 2q23.1

Cellular Component: nucleus; chromocenter

Molecular Function: DNA binding; chromatin binding

Biological Process: nervous system development; regulation of multicellular organism growth; glucose homeostasis

Disease: Mental Retardation, Autosomal Dominant 1
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Research Articles on MBD5

1. study demonstrates that haploinsufficiency of MBD5 causes diverse phenotypes, yields insight into the spectrum of resulting neurodevelopmental and behavioral psychopathology and provides clinical context for interpretation of MBD5 structural variations.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-MBD5 antibody

 Disease Name  Pubmed Publications
 Congenital Abnormalities Antibodies  >14 publications with MBD5 and Congenital Abnormalities
 Musculoskeletal Diseases Antibodies  >7 publications with MBD5 and Musculoskeletal Diseases
 Cardiovascular Diseases Antibodies  >1 publications with MBD5 and Cardiovascular Diseases
 Weight Gain Antibodies  >1 publications with MBD5 and Weight Gain
 Abnormalities, Drug-Induced Antibodies  >1 publications with MBD5 and Abnormalities, Drug-Induced
 Heart Diseases Antibodies  >1 publications with MBD5 and Heart Diseases
 Liver Diseases Antibodies  >1 publications with MBD5 and Liver Diseases

Organs/Tissues associated with anti-MBD5 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >10 publications with MBD5 and Brain
 Mouth Antibodies  >2 publications with MBD5 and Mouth
 Muscle Antibodies  >1 publications with MBD5 and Muscle
 Eye Antibodies  >1 publications with MBD5 and Eye
 Intestine Antibodies  >1 publications with MBD5 and Intestine
 Liver Antibodies  >1 publications with MBD5 and Liver
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