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MCM2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6319665

MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MCM2, Polyclonal Antibody
Full Product Name
MCM2, CT (MCM2, BM28, CCNL1, CDCL1, KIAA0030, DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28) (Azide free) (HRP)
Product Gene Name
anti-MCM2 antibody
Product Synonym Names
MCM2; BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
101,896 Da
Chromosome Location
Chromosome: 3; NC_000003.11 (127317253..127341279). Location: 3q21
OMIM
116945
3D Structure
ModBase 3D Structure for P49736
Immunogen
MCM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656~685 amino acids from the C-terminal region of human MCM2.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-MCM2 antibody

Antibodies; Cell Division Cycle (CDC)
Applications Tested/Suitable for anti-MCM2 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-MCM2 antibody
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MCM2 antibody

Western blot analysis of MCM2 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). MCM2 (arrow) was detected using the purified Pab.
Rabbit Anti-MCM2 Polyclonal Antibody – Cat# MBS6319665

Immunohistochemistry (IHC) of anti-MCM2 antibody

MCM2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCM2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-MCM2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6319665

Product References and Citations for anti-MCM2 antibody

Saade, E., et al. Proteomics 9(21):4934-4943(2009). Xu, X., et al. EMBO J. 28(19):3005-3014(2009). Liaw, K., et al. J. Cutan. Pathol. 36(10):1121-1122(2009). Im, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 106(37):15628-15632(2009). Chuang, L.C., et al. Mol. Cell 35(2):206-216(2009). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006).

Other Notes

Small volumes of anti-MCM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MCM2 purchase

MBS6319665 is an antibody targeting MCM2. Also known as MCM2, CT (MCM2, BM28, CCNL1, CDCL1, KIAA0030, DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28) (Azide free) (HRP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MCM2. It may not necessarily be applicable to this product.

NCBI GI #
33356547
NCBI GeneID
4171
NCBI Accession #
NP_004517.2
UniProt Primary Accession #
P49736
UniProt Secondary Accession #
Q14577; Q15023; Q8N2V1; Q969W7; Q96AE1; Q9BRM7
UniProt Related Accession #
P49736
GenBank Nucleotide #
NM_004526.3
NCBI Official Full Name
DNA replication licensing factor MCM2
NCBI Official Synonym Full Names
minichromosome maintenance complex component 2
NCBI Official Symbol
MCM2
NCBI Official Synonym Symbols
BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN
UniProt Protein Name
DNA replication licensing factor MCM2
UniProt Synonym Protein Names
Minichromosome maintenance protein 2 homolog; Nuclear protein BM28
Protein Family
DNA replication licensing factor
UniProt Gene Name
MCM2
UniProt Synonym Gene Names
BM28; CCNL1; CDCL1; KIAA0030
UniProt Entry Name
MCM2_HUMAN
NCBI Protein Information
DNA replication licensing factor MCM2; cyclin-like 1; nuclear protein BM28; cell devision cycle-like 1; minichromosome maintenance protein 2 homolog; minichromosome maintenance deficient 2 (mitotin); MCM2 minichromosome maintenance deficient 2, mitotin

NCBI Summary for MCM2

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]
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UniProt Comments for MCM2

MCM2: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division.

Protein type: DNA-binding; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: microtubule cytoskeleton; nucleoplasm; MCM complex; cytoplasm; chromatin; nuclear origin of replication recognition complex; nucleus

Molecular Function: protein binding; DNA helicase activity; DNA binding; histone binding; metal ion binding; DNA replication origin binding; ATP binding

Biological Process: DNA unwinding during replication; nucleosome assembly; DNA replication initiation; DNA strand elongation during DNA replication; mitotic cell cycle; cell cycle; DNA replication; G1/S transition of mitotic cell cycle
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Research Articles on MCM2

1. Immunohistochemical expression of minichromosome maintenance complex protein 2 predicts biochemical recurrence in prostate cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MCM2 antibody

 Reference Product  PubMed Publications
 CDC6 antibody  >106 publications with MCM2 and CDC6
 MCM7 antibody  >99 publications with MCM2 and MCM7
 MCM4 antibody  >95 publications with MCM2 and MCM4
 MCM5 antibody  >88 publications with MCM2 and MCM5
 MCM3 antibody  >88 publications with MCM2 and MCM3
 MCM6 antibody  >77 publications with MCM2 and MCM6
 CDC7 antibody  >63 publications with MCM2 and CDC7
 DBF4 antibody  >41 publications with MCM2 and DBF4
 MCM10 antibody  >39 publications with MCM2 and MCM10
 ORC2 antibody  >18 publications with MCM2 and ORC2

Pathways associated with anti-MCM2 antibody

 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Assembly Of The Pre-replicative Complex Pathway antibodies  Assembly Of The Pre-replicative Complex Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram

Diseases associated with anti-MCM2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >225 publications with MCM2 and Neoplasms
 Carcinoma Antibodies  >132 publications with MCM2 and Carcinoma
 Adenocarcinoma Antibodies  >55 publications with MCM2 and Adenocarcinoma
 Uterine Cervical Neoplasms Antibodies  >29 publications with MCM2 and Uterine Cervical Neoplasms
 Precancerous Conditions Antibodies  >23 publications with MCM2 and Precancerous Conditions
 Nervous System Diseases Antibodies  >19 publications with MCM2 and Nervous System Diseases
 Breast Neoplasms Antibodies  >18 publications with MCM2 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >17 publications with MCM2 and Cell Transformation, Neoplastic
 Brain Diseases Antibodies  >15 publications with MCM2 and Brain Diseases
 Neoplasms, Experimental Antibodies  >14 publications with MCM2 and Neoplasms, Experimental

Organs/Tissues associated with anti-MCM2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >26 publications with MCM2 and Brain
 Blood Antibodies  >24 publications with MCM2 and Blood
 Lymph Node Antibodies  >22 publications with MCM2 and Lymph Node
 Lymph Antibodies  >22 publications with MCM2 and Lymph
 Lung Antibodies  >20 publications with MCM2 and Lung
 Liver Antibodies  >16 publications with MCM2 and Liver
 Cervix Antibodies  >14 publications with MCM2 and Cervix
 Bone Antibodies  >14 publications with MCM2 and Bone
 Prostate Antibodies  >12 publications with MCM2 and Prostate
 Mouth Antibodies  >10 publications with MCM2 and Mouth
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