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POLE3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6494107

MBS6494107 is an antibody targeting POLE3. Also known as POLE3, NT (DNA Polymerase epsilon Subunit 3, DNA Polymerase II Subunit 3, DNA Polymerase epsilon Subunit p17, Chromatin Accessibility Complex 17kD Protein, HuCHRAC17, CHRAC-17, Arsenic-transactivated… More details

$1060 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
POLE3, Polyclonal Antibody
Full Product Name
POLE3, NT (DNA Polymerase epsilon Subunit 3, DNA Polymerase II Subunit 3, DNA Polymerase epsilon Subunit p17, Chromatin Accessibility Complex 17kD Protein, HuCHRAC17, CHRAC-17, Arsenic-transactivated Protein, AsTP, CHRAC17) (MaxLight 650)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human POLE3.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
19kDa (170aa), confirmed by MALDI-TOF
OMIM
607267
3D Structure
ModBase 3D Structure for Q9NRF9
Immunogen
Synthetic peptide selected from the N-terminal region of human POLE3 (KLH).
Conjugate
MaxLight650
Note
Preservative Free

Product Categories/Family for anti-POLE3 antibody

Applications Tested/Suitable for anti-POLE3 antibody
Western Blot (WB), FLISA
Application Notes for anti-POLE3 antibody
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-POLE3 antibody

POLE3 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the POLE3 antibody detected the POLE3 protein (arrow).
Rabbit Anti-POLE3 Polyclonal Antibody – Cat# MBS6494107

Flow Cytometry (FC/FACS) of anti-POLE3 antibody

POLE3 Antibody (N-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-POLE3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6494107

Product References and Citations for anti-POLE3 antibody

1. Bolognese, F., Imbriano, C. Nucleic Acids Res. 28(19), 3830-3838 (2000). 2. Li, Y., Pursell, Z.F. J. Biol. Chem. 275(30), 23247-23252 (2000).

Other Notes

Small volumes of anti-POLE3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for POLE3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA polymerase epsilon subunit 3
NCBI Official Synonym Full Names
DNA polymerase epsilon 3, accessory subunit
NCBI Official Symbol
POLE3
NCBI Official Synonym Symbols
p17; YBL1; CHRAC17; CHARAC17
UniProt Protein Name
DNA polymerase epsilon subunit 3
UniProt Synonym Protein Names
Arsenic-transactivated protein; AsTP; Chromatin accessibility complex 17 kDa protein; CHRAC-17; HuCHRAC17; DNA polymerase II subunit 3; DNA polymerase epsilon subunit p17
Protein Family
UniProt Gene Name
POLE3
UniProt Synonym Gene Names
CHRAC17; AsTP; CHRAC-17; HuCHRAC17
UniProt Entry Name
DPOE3_HUMAN
NCBI Protein Information
DNA polymerase epsilon subunit 3

NCBI Summary for POLE3

POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004]

UniProt Comments for POLE3

POLE3: Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.

Protein type: EC 2.7.7.7; DNA replication; Nucleotide Metabolism - purine; Transferase; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: epsilon DNA polymerase complex; nucleus

Molecular Function: protein binding; protein heterodimerization activity; sequence-specific DNA binding; DNA-directed DNA polymerase activity

Biological Process: DNA replication
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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