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PPM1N Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1759493

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PPM1N, Polyclonal Antibody
Full Product Name
Anti-PPM1N Antibody Picoband
Product Gene Name
anti-PPM1N antibody
Product Synonym Names
Transmembrane protein 240; kelch repeat and BTB domain containing 2; Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
11,674 Da
3D Structure
ModBase 3D Structure for Q8N819
Immunogen
E coli-derived human PPM1N recombinant protein (Position: R36-A430).
Subcellular Localization
Cul3-RING ubiquitin ligase complex
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

Product Categories/Family for anti-PPM1N antibody

Primary Antibodies; Cell Biology
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PPM1N antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-PPM1N antibody
WB: 0.25-0.5ug/ml, Human
ICC/IF: 5ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-PPM1N antibody

Figure 1. Western blot analysis of PPM1N using anti-PPM1N antibody (MBS1759493).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPM1N antigen affinity purified polyclonal antibody (#MBS1759493) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for PPM1N at approximately 45 kDa. The expected band size for PPM1N is at 46 kDa.
Rabbit Anti-PPM1N Polyclonal Antibody – Cat# MBS1759493

Immunofluorescence (IF) of anti-PPM1N antibody

Figure 2. IF analysis of PPM1N using anti-PPM1N antibody (MBS1759493).
PPM1N was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (#MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-PPM1N Antibody (MBS1759493) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-PPM1N Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1759493

Flow Cytometry (FC/FACS) of anti-PPM1N antibody

Figure 3. Flow Cytometry analysis of MCF-7 cells using anti-PPM1N antibody (MBS1759493).
Overlay histogram showing MCF-7 cells stained with MBS1759493 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPM1N Antibody (MBS1759493, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-PPM1N Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1759493

Product References and Citations for anti-PPM1N antibody

1. Zhou, H. , Xu, M. , Huang, Q. , Gates, A. T. , Zhang, X. D. , & Castle, J. C. , et al. (2008). Genome-scale rnai screen for host factors required for hiv replication. Cell host & microbe, 4(5), 495-504.2. Shi, P. , Guo, Y. , Su, Y. , Zhu, M. , & Huang, J. . (2020). Sumoylation of ddx39a alters binding and export of antiviral transcripts to control innate immunity. The Journal of Immunology, 205(1), ji2000053.3. Fasci, D. , Ingen, H. V. , Scheltema, R. A. , & Heck, A. J. R. . (2018). Histone interaction landscapes visualized by crosslinking mass spectrometry in intact cell nuclei. Molecular & Cellular Proteomics Mcp, 17(10), mcp.RA118.000924.

Other Notes

Small volumes of anti-PPM1N antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPM1N purchase

MBS1759493 is an antibody targeting PPM1N. Also known as Anti-PPM1N Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PPM1N. It may not necessarily be applicable to this product.

NCBI GI #
122937199
NCBI GeneID
147699
NCBI Accession #
NP_001073870.1
UniProt Primary Accession #
Q8N819
UniProt Secondary Accession #
Q6P662
UniProt Related Accession #
Q8N819
GenBank Nucleotide #
NM_001080401.1
NCBI Official Full Name
probable protein phosphatase 1N
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent 1N (putative)
NCBI Official Symbol
PPM1N
UniProt Protein Name
Probable protein phosphatase 1N
Protein Family
Probable protein phosphatase
UniProt Gene Name
PPM1N
NCBI Protein Information
probable protein phosphatase 1N

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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