Mouse Anti-PREX2 Polyclonal Antibody – Cat# MBS648052

Western Blot analysis of PREX2 expression in transfected 293T cell line by PREX2 polyclonal antibody. Lane 1: DEPDC2 transfected lysate (107.69kD). Lane 2: Non-transfected lysate.

PREX2 Antibody – Mouse Polyclonal

Name: PREX2 Antibody; Polyclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS648052
Price: 880 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS648052

Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G… More details

$880 / 0.05 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PREX2, Polyclonal Antibody

Full Product Name

PREX2 (DEPDC2, Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac Exchanger 2 Protein, P-Rex2, PtdIns(3,4,5)-dependent Rac Exchanger 2, DEP Domain-containing Protein 2, 6230420N16Rik, FLJ12987)

Product Gene Name

anti-PREX2 antibody

Product Synonym Names

Anti -PREX2 (DEPDC2, Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac Exchanger 2 Protein, P-Rex2, PtdIns(3,4,5)-dependent Rac Exchanger 2, DEP Domain-containing Protein 2, 6230420N16Rik, FLJ12987)

Immunogen Sequence

MSEDSRGDSR AESAKDLEKQ LRLRVCVLSE LQKTERDYVG TLEFLVSAFL HRMNQCAASK VDKNVTEETV KMLFSNIEDI LAVHKEFLKV VEECLHPEPN AQQEVGTCFL HFKDKFRIYD EYCSNHEKAQ KLLLELNKIR TIRTFLLNCM LLGGRKNTDV PLEGYLVTPI QRICKYPLIL KELLKRTPRK HSDYAAVMEA LQAMKAVCSN INEAKRQMEK LEVLEEWQSH IEGWEGSNIT DTCTEMLMCG VLLKISSGNI QERVFFLFDN LLVYCKRKHR RLKNSKASTD GHRYLFRGRI NTEVMEVENV DDGTADFHSS GHIVVNGWKI HNTAKNKWFV CMAKTPEEKH EWFEAILKER ERRKGLKLGM EQDTWVMISE QGEKLYKMMC RQGNLIKDRK RKLTTFPKCF LGSEFVSWLL EIGEIHRPEE GVHLGQALLE NGIIHHVTDK HQFKPEQMLY RFRYDDGTFY PRNEMQDVIS KGVRLYCRLH SLFTPVIRDK DYHLRTYKSV VMANKLIDWL IAQGDCRTRE EAMIFGVGLC DNGFMHHVLE KSEFKDEPLL FRFFSDEEME GSNMKHRLMK HDLKVVENVI AKSLLIKSNE GSYGFGLEDK NKVPIIKLVE KGSNAEMAGM EVGKKIFAIN GDLVFMRPFN EVDCFLKSCL NSRKPLRVLV STKPRETVKI PDSADGLGFQ IRGFGPSVVH AVGRGTVAAA AGLHPGQCII KVNGINVSKE THASVIAHVT ACRKYRRPTK QDSIQWVYNS IESAQEDLQK SHSKPPGDEA GDAFDCKVEE VIDKFNTMAI IDGKKEHVSL TVDNVHLEYG VVYEYDSTAG IKCNVVEKMI EPKGFFSLTA KILEALAKSD EHFVQNCTSL NSLNEVIPTD LQSKFSALCS ERIEHLCQRI SSYKKVQASE RFYNFTARHA VWEHSFDLHS VSSTFPVPVT MEFLLLPPPL LGISQDGRQH CIPEDLPSQE MLLAERAPV

Clonality

Polyclonal

Isotype

IgG

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography.

Specificity

Recognizes human DEPDC2.

Preparation and Storage

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight

182,622 Da

Chromosome Location

Chromosome: 8; NC_000008.10 (68864244..69143897). Location: 8q13.2

OMIM

612139

3D Structure

ModBase 3D Structure for Q70Z35

Immunogen

Full length human DEPDC2, aa1-979 (AAI48796.1).

Product Categories/Family for anti-PREX2 antibody

Antibodies; Abs to Signal Transduction

Applications Tested/Suitable for anti-PREX2 antibody

Western Blot (WB)

Application Notes for anti-PREX2 antibody

Suitable for use in Western Blot.

Other Notes

Small volumes of anti-PREX2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PREX2 purchase

MBS648052 is an antibody targeting PREX2. Also known as PREX2 (DEPDC2, Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac Exchanger 2 Protein, P-Rex2, PtdIns(3,4,5)-dependent Rac Exchanger 2, DEP Domain-containing Protein 2, 6230420N16Rik, FLJ12987). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PREX2. It may not necessarily be applicable to this product.

NCBI GI #

47578117

NCBI GeneID

80243

NCBI Accession #

NP_079446.3

UniProt Primary Accession #

Q70Z35

UniProt Secondary Accession #

Q32KL0; Q32KL1; Q6R7Q3; Q6R7Q4; Q9H805; Q9H961

UniProt Related Accession #

Q70Z35

GenBank Nucleotide #

NM_025170.4

NCBI Official Full Name

phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein isoform b

NCBI Official Synonym Full Names

phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2

NCBI Official Symbol

PREX2

NCBI Official Synonym Symbols

DEP.2; DEPDC2; P-REX2; 6230420N16Rik

UniProt Protein Name

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein

UniProt Synonym Protein Names

DEP domain-containing protein 2

Protein Family

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein

UniProt Gene Name

PREX2

UniProt Synonym Gene Names

DEPDC2; P-Rex2

UniProt Entry Name

PREX2_HUMAN

NCBI Protein Information

phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein; DEP domain-containing protein 2; PtdIns(3,4,5)-dependent Rac exchanger 2

UniProt Comments for PREX2

Function: Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase. Ref.1 Ref.5 Ref.6

Subunit structure: Interacts with RAC1.

Tissue specificity: Isoform 1 is highly expressed in skeletal muscle, heart and placenta, absent from peripheral blood leukocytes. Isoform 2 is expressed in skeletal muscle, kidney, small intestine, and placenta. Isoform 3 is expressed in the heart. Ref.1

Domain: PH domain confers substrate specificity and recognition. Able to discriminate between RAC1, RHOA, and CDC42.DH domain alone was unable to confer substrate specificity and recognition.

Sequence similarities: Contains 2 DEP domains.Contains 1 DH (DBL-homology) domain.Contains 2 PDZ (DHR) domains.Contains 1 PH domain.

Sequence caution: The sequence AK024079 differs from that shown. Reason: Frameshift at position 1134. The sequence BAB14375.1 differs from that shown. Reason: Erroneous initiation.

Research Articles on PREX2

1. Studies indicate relevance of P-Rex1 and P-Rex2a, in breast tumorigenesis, and suggest that the exchange factors Vav2 and Vav3 play synergistic roles in breast cancer by sustaining tumor growth, neoangiogenesis, and metastasis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PREX2 antibody

Reference Product	PubMed Publications
RAC1 antibody	>5 publications with PREX2 and RAC1

Pathways associated with anti-PREX2 antibody

Products by Pathway	Pathway Diagram
Regulation Of RAC1 Activity Pathway antibodies	Regulation Of RAC1 Activity Pathway Diagram

Diseases associated with anti-PREX2 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>6 publications with PREX2 and Neoplasms

Organs/Tissues associated with anti-PREX2 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1 publications with PREX2 and Blood
Kidney Antibodies	>1 publications with PREX2 and Kidney
Vascular Antibodies	>1 publications with PREX2 and Vascular
Lung Antibodies	>1 publications with PREX2 and Lung
Testis Antibodies	>1 publications with PREX2 and Testis
Embryonic Tissue Antibodies	>1 publications with PREX2 and Embryonic Tissue