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RECK Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758715

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RECK, Polyclonal Antibody
Full Product Name
Anti-RECK Antibody Picoband
Product Gene Name
anti-RECK antibody
Product Synonym Names
Serine/threonine-protein kinase AtPK1/AtPK6; 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Expressed in all tissues.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
971
Chromosome Location
Chromosome: 9; NC_000009.11 (36036910..36124452). Location: 9p13.3
OMIM
605227
3D Structure
ModBase 3D Structure for O95980
Immunogen
E coli-derived human RECK recombinant protein (Position: N371-A414).
Subcellular Localization
Cytoplasm, Nucleus
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross reactivity with other proteins.
Protein Function
Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.

Product Categories/Family for anti-RECK antibody

Primary Antibodies; Signal Transduction; Signaling Pathway; Small G Proteins
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-RECK antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-RECK antibody
WB: 0.1-0.25ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-RECK antibody

Figure 1. Western blot analysis of RECK using anti-RECK antibody (MBS1758715).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RECK antigen affinity purified polyclonal antibody (#MBS1758715) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for RECK at approximately 106 kDa. The expected band size for RECK is at 106 kDa.
Rabbit Anti-RECK Polyclonal Antibody – Cat# MBS1758715

Flow Cytometry (FC/FACS) of anti-RECK antibody

Figure 2. Flow Cytometry analysis of U251 cells using anti-RECK antibody (MBS1758715).
Overlay histogram showing U251 cells stained with MBS1758715 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-RECK Antibody (MBS1758715, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-RECK Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758715

Product References and Citations for anti-RECK antibody

1. "Entrez Gene: RECK reversion-inducing-cysteine-rich protein with kazal motifs".2. Park, S., Lee, C., Sabharwal, P., Zhang, M., Meyers, C. L. F., Sockanathan, S. GDE2 promotes neurogenesis by glycosylphosphatidylinositol-anchor cleavage of RECK. Science 339: 324-328, 2013.

Other Notes

Small volumes of anti-RECK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RECK purchase

MBS1758715 is an antibody targeting RECK. Also known as Anti-RECK Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RECK. It may not necessarily be applicable to this product.

NCBI GI #
11863156
NCBI GeneID
8434
NCBI Accession #
NP_066934.1
UniProt Primary Accession #
O95980
UniProt Secondary Accession #
Q5W0K6; Q8WX37; B2RNS1
UniProt Related Accession #
O95980
GenBank Nucleotide #
NM_021111.2
NCBI Official Full Name
reversion-inducing cysteine-rich protein with Kazal motifs
NCBI Official Synonym Full Names
reversion-inducing-cysteine-rich protein with kazal motifs
NCBI Official Symbol
RECK
NCBI Official Synonym Symbols
ST15
UniProt Protein Name
Reversion-inducing cysteine-rich protein with Kazal motifs
UniProt Synonym Protein Names
Suppressor of tumorigenicity 15 protein
Protein Family
Reversion-inducing cysteine-rich protein
UniProt Gene Name
RECK
UniProt Synonym Gene Names
ST15; hRECK
UniProt Entry Name
RECK_HUMAN
NCBI Protein Information
reversion-inducing cysteine-rich protein with Kazal motifs; suppressor of tumorigenicity 15 protein; membrane-anchored glycoprotein (metastasis and invasion); suppression of tumorigenicity 5 (reversion-inducing-cysteine-rich protein with kazal motifs); su

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RECK antibody

 Reference Product  PubMed Publications
 MMP9 antibody  >13 publications with RECK and MMP9
 MMP2 antibody  >10 publications with RECK and MMP2
 MMP14 antibody  >7 publications with RECK and MMP14
 TIMP2 antibody  >5 publications with RECK and TIMP2
 ERBB2 antibody  >4 publications with RECK and ERBB2
 HIF1A antibody  >3 publications with RECK and HIF1A
 ANGPT1 antibody  >1 publications with RECK and ANGPT1

Pathways associated with anti-RECK antibody

 Products by Pathway  Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram

Diseases associated with anti-RECK antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >325 publications with RECK and Neoplasms
 Lung Neoplasms Antibodies  >128 publications with RECK and Lung Neoplasms
 Carcinoma, Non-Small-Cell Lung Antibodies  >96 publications with RECK and Carcinoma, Non-Small-Cell Lung
 Neoplasm Metastasis Antibodies  >68 publications with RECK and Neoplasm Metastasis
 Nervous System Diseases Antibodies  >66 publications with RECK and Nervous System Diseases
 Neoplasm Invasiveness Antibodies  >55 publications with RECK and Neoplasm Invasiveness
 Adenocarcinoma Antibodies  >45 publications with RECK and Adenocarcinoma
 Drug Toxicity Antibodies  >42 publications with RECK and Drug Toxicity
 Brain Diseases Antibodies  >30 publications with RECK and Brain Diseases
 Neoplasms, Experimental Antibodies  >23 publications with RECK and Neoplasms, Experimental
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