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SA1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3900801

SA1 is a component of the multisubunit cohesin complex. Cohesin functions to hold sister chromatids together during meiosis and mitosis and ensure proper segregation of chromosomes. SA1 has also been identified as a mediator of p53 induced apoptosis. More details

$250 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SA1, Polyclonal Antibody
Full Product Name
Goat anti-SA1 Antibody, Affinity Purified
Product Gene Name
Product Synonym Gene Name
STAG1
Product Synonym Names
MRD47; Stromal antigen 1; SCC3A; SCC3 homolog 1; nuclear protein stromal antigen 1; cohesin subunit SA-1; SA1; Cohesin subunit SA-1; stromal antigen 1
Clonality
Polyclonal
Host
Goat
Species Reactivity
Human
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
140,090 Da
OMIM
604358
3D Structure
ModBase 3D Structure for Q8WVM7
Immunogen
Between 1150 and 1200
Conjugation
Unconjugated

Product Categories/Family for anti-SA1 antibody

Applications Tested/Suitable for anti-SA1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes for anti-SA1 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
ICC: 1:500-1:2000

Immunocytochemistry (ICC) of anti-SA1 antibody

Localization of human SA1. Sample: HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody: Affinity purified goat anti-SA1 (BL143G; MBS3900801) used at 1 ug/ml.
Goat Anti-SA1 Polyclonal Antibody – Cat# MBS3900801

Immunoprecipitation (IP) of anti-SA1 antibody

Detection of human SA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-SA1 antibody MBS3900801 (lot MBS3900801-3) used for IP at 6 ug per reaction. SA1 was also immunoprecipitated by a previous lot of this antibody (lot MBS3900801-2). For blotting immunoprecipitated SA1, MBS3900801 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Goat Anti-SA1 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3900801

Western Blot (WB) of anti-SA1 antibody

Detection of human SA1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-SA1 antibody MBS3900801 (lot MBS3900801-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Goat Anti-SA1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3900801

Other Notes

Small volumes of anti-SA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SA1 purchase

MBS3900801 is an antibody targeting SA1. Also known as Goat anti-SA1 Antibody, Affinity Purified. Reactive in Human. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for SA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cohesin subunit SA-1
NCBI Official Synonym Full Names
stromal antigen 1
NCBI Official Symbol
STAG1
NCBI Official Synonym Symbols
SA1; SCC3A
UniProt Protein Name
Cohesin subunit SA-1
UniProt Synonym Protein Names
SCC3 homolog 1; Stromal antigen 1
UniProt Gene Name
STAG1
UniProt Synonym Gene Names
SA1
UniProt Entry Name
STAG1_HUMAN
NCBI Protein Information
cohesin subunit SA-1; SCC3 homolog 1; nuclear protein stromal antigen 1

NCBI Summary for SA1

This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase. [provided by RefSeq, Jul 2008]
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UniProt Comments for SA1

STAG1: Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Belongs to the SCC3 family.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 3q22.3

Cellular Component: nucleoplasm; chromosome; nucleus; cytosol; chromatin; chromosome, pericentric region

Molecular Function: protein binding; chromatin binding

Biological Process: mitosis; cell division; mitotic cell cycle; chromosome segregation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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