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SOHLH1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9207236

Probable transcription factor required during spermatogenesis and oogenesis (By similarity). More details

$240 / 0.08 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SOHLH1, Polyclonal Antibody
Full Product Name
SOHLH1 Antibody (N-term)
Product Gene Name
Product Synonym Names
Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1; SOHLH1; C9orf157; NOHLH; TEB2
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Concentration
Vial Concentration: 0.5 (lot specific)
Specificity
This SOHLH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-68 amino acids from the N-terminal region of human SOHLH1.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
SOHLH1 peptide (MBS9217979) is used for blocking the activity of SOHLH1 antibody (MBS9207236)
Molecular Weight
34526
Immunogen Sequence Positions
40-68
OMIM
610224
3D Structure
ModBase 3D Structure for Q5JUK2
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Applications Tested/Suitable for anti-SOHLH1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-SOHLH1 antibody
WB~~1:1000

Western Blot (WB) of anti-SOHLH1 antibody

SOHLH1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SOHLH1 antibody detected the SOHLH1 protein (arrow).
Rabbit Anti-SOHLH1 Polyclonal Antibody – Cat# MBS9207236

Immunohistochemistry (IHC) of anti-SOHLH1 antibody

SOHLH1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SOHLH1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-SOHLH1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9207236

Flow Cytometry (FC/FACS) of anti-SOHLH1 antibody

SOHLH1 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-SOHLH1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS9207236

Product References and Citations for anti-SOHLH1 antibody

Choi, Y., et al. Hum. Mutat. 31(7):788-793(2010)
Pangas, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 103(21):8090-8095(2006)

Other Notes

Small volumes of anti-SOHLH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SOHLH1 purchase

MBS9207236 is an antibody targeting SOHLH1. Also known as SOHLH1 Antibody (N-term). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SOHLH1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1 isoform b
NCBI Official Synonym Full Names
spermatogenesis and oogenesis specific basic helix-loop-helix 1
NCBI Official Symbol
SOHLH1
NCBI Official Synonym Symbols
TEB2; NOHLH; SPATA27; bHLHe80; C9orf157; bA100C15.3
UniProt Protein Name
Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1
UniProt Gene Name
SOHLH1
UniProt Synonym Gene Names
C9orf157; NOHLH; TEB2
UniProt Entry Name
SOLH1_HUMAN
NCBI Protein Information
spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1

NCBI Summary for SOHLH1

This gene encodes one of testis-specific transcription factors which are essential for spermatogenesis, oogenesis and folliculogenesis. This gene is located on chromosome 9. Mutations in this gene are associated with nonobstructive azoospermia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2013]
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UniProt Comments for SOHLH1

SOHLH1: Transcription factor expressed in undifferentiated spermatogonia required for spermatogonial development. Genetic variations in SOHLH1 may be associated with non-obstructive azoospermia. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein dimerization activity; DNA binding; transcription factor activity

Biological Process: oogenesis; ovarian follicle development; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; spermatogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-SOHLH1 antibody

Organs/Tissues associated with anti-SOHLH1 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >10 publications with SOHLH1 and Testis
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