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TLE6 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6355813

As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions (By similarity). More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TLE6, Polyclonal Antibody
Full Product Name
TLE6, NT (TLE6, Transducin-like enhancer protein 6) (Biotin)
Product Gene Name
anti-TLE6 antibody
Product Synonym Names
TLE6; Transducin-like enhancer protein 6
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
OMIM
612399
3D Structure
ModBase 3D Structure for Q9H808
Immunogen
TLE6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human TLE6.
Conjugate
Biotin
Note
Preservative Free

Product Categories/Family for anti-TLE6 antibody

Antibodies; Neuroscience
Applications Tested/Suitable for anti-TLE6 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-TLE6 antibody
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-TLE6 antibody

TLE6 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TLE6 antibody detected the TLE6 protein (arrow).
Rabbit Anti-TLE6 Polyclonal Antibody – Cat# MBS6355813

Immunohistochemistry (IHC) of anti-TLE6 antibody

TLE6 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TLE6 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-TLE6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6355813

Product References and Citations for anti-TLE6 antibody

Chen, P.C., et al. PLoS Genet. 4 (6), E1000092 (2008): Dang, J., et al. Mol. Cell. Biol. 21(17):5935-5945(2001).

Other Notes

Small volumes of anti-TLE6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TLE6 purchase

MBS6355813 is an antibody targeting TLE6. Also known as TLE6, NT (TLE6, Transducin-like enhancer protein 6) (Biotin). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TLE6. It may not necessarily be applicable to this product.

NCBI GI #
13376099
NCBI GeneID
79816
NCBI Accession #
NP_079036.1
NCBI Related Accession #
NP_001137458.1
UniProt Primary Accession #
Q9H808
UniProt Related Accession #
Q9H808
GenBank Nucleotide #
NP_079036.1
NCBI Official Full Name
transducin-like enhancer protein 6 isoform 2
NCBI Official Synonym Full Names
TLE family member 6, subcortical maternal complex member
NCBI Official Symbol
TLE6
NCBI Official Synonym Symbols
GRG6; PREMBL
UniProt Protein Name
Transducin-like enhancer protein 6
Protein Family
Transducin-like enhancer protein
UniProt Gene Name
TLE6
UniProt Entry Name
TLE6_HUMAN
NCBI Protein Information
transducin-like enhancer protein 6

NCBI Summary for TLE6

This gene encodes a member of the Groucho/ transducin-like Enhancer of split family of transcriptional co-repressors. The encoded protein is a component of the mammalian subcortical maternal complex, which is required for preimplantation development. In mouse, knock out of this gene results in cleavage-stage embryonic arrest resulting from defective cytoplasmic F-actin meshwork formation and asymmetric cell division. In human, an allelic variant in this gene is associated with preimplantation embryonic lethality. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
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UniProt Comments for TLE6

TLE6: As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions. Belongs to the WD repeat Groucho/TLE family.

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: protein complex; cell cortex; nucleus

Biological Process: regulation of transcription, DNA-dependent; positive regulation of neuron differentiation
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Research Articles on TLE6

1. In this first report of a human defect in a member of the subcortical maternal subcritical maternal complex, we show that the TLE6 mutation is gender-specific and leads to the earliest known human embryonic lethality phenotype

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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