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BUB3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS821647

MBS821647 is an antibody targeting BUB3. Reactive in Human, Monkey. Host: Rabbit. Rabbit polyclonal antibody to BUB3 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
BUB3, Polyclonal Antibody
Full Product Name
Anti-BUB3 Antibody
Product Gene Name
anti-BUB3 antibody
Product Synonym Names
Mitotic checkpoint protein BUB3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Monkey
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of BUB3 protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
BUB3 peptide (MBS823216) is used for blocking the activity of BUB3 antibody (MBS821647)
Molecular Weight
36,955 Da
OMIM
603719
3D Structure
ModBase 3D Structure for O43684
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human BUB3. The exact sequence is proprietary.
Applications Tested/Suitable for anti-BUB3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes for anti-BUB3 antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500), IP (1/10 - 1/100)

Western Blot (WB) of anti-BUB3 antibody

Western blot analysis of BUB3 expression in HeLa (A), HEK293T (B) whole cell lysates.
Rabbit Anti-BUB3 Polyclonal Antibody – Cat# MBS821647

Immunohistochemistry (IHC) of anti-BUB3 antibody

Immunohistochemical analysis of BUB3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-BUB3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS821647

Immunofluorescence (IF) of anti-BUB3 antibody

Immunofluorescent analysis of BUB3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-BUB3 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS821647

Other Notes

Small volumes of anti-BUB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BUB3. It may not necessarily be applicable to this product.

NCBI GI #
56550081
NCBI GeneID
9184
NCBI Accession #
NP_001007794.1
UniProt Primary Accession #
O43684
UniProt Secondary Accession #
O43685; A6NJ42; B2R6E7; D3DRE9
UniProt Related Accession #
O43684
GenBank Nucleotide #
NM_001007793.2
NCBI Official Full Name
mitotic checkpoint protein BUB3 isoform b
NCBI Official Synonym Full Names
BUB3 mitotic checkpoint protein
NCBI Official Symbol
BUB3
NCBI Official Synonym Symbols
BUB3L; hBUB3
UniProt Protein Name
Mitotic checkpoint protein BUB3
Protein Family
Cell cycle arrest protein
UniProt Gene Name
BUB3
UniProt Entry Name
BUB3_HUMAN
NCBI Protein Information
mitotic checkpoint protein BUB3; mitotic checkpoint component; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog; BUB3 budding uninhibited by benzimidazoles 3 homolog

NCBI Summary for BUB3

This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for BUB3

BUB3: Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Belongs to the WD repeat BUB3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: nucleoplasm; kinetochore; cytosol

Molecular Function: protein binding

Biological Process: negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; meiotic cell cycle; mitotic sister chromatid segregation; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; attachment of spindle microtubules to kinetochore
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Research Articles on BUB3

1. Results showed that neither variants in BUB3 nor variants in MAD2L1 caused any significant effect on the risk of breast cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BUB3 antibody

 Reference Product  PubMed Publications
 CDC20 antibody  >51 publications with BUB3 and CDC20
 BUB1B antibody  >9 publications with BUB3 and BUB1B
 ZNF207 antibody  >2 publications with BUB3 and ZNF207
 CDC27 antibody  >2 publications with BUB3 and CDC27
 CDC16 antibody  >1 publications with BUB3 and CDC16

Pathways associated with anti-BUB3 antibody

 Products by Pathway  Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram

Diseases associated with anti-BUB3 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >12 publications with BUB3 and Carcinoma
 Adenocarcinoma Antibodies  >7 publications with BUB3 and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >7 publications with BUB3 and Cell Transformation, Neoplastic
 Nervous System Diseases Antibodies  >5 publications with BUB3 and Nervous System Diseases
 Carcinoma, Hepatocellular Antibodies  >4 publications with BUB3 and Carcinoma, Hepatocellular
 Liver Diseases Antibodies  >3 publications with BUB3 and Liver Diseases
 Liver Neoplasms Antibodies  >3 publications with BUB3 and Liver Neoplasms
 Ovarian Neoplasms Antibodies  >3 publications with BUB3 and Ovarian Neoplasms
 Kidney Neoplasms Antibodies  >2 publications with BUB3 and Kidney Neoplasms
 Neoplasm Metastasis Antibodies  >2 publications with BUB3 and Neoplasm Metastasis

Organs/Tissues associated with anti-BUB3 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >6 publications with BUB3 and Lung
 Brain Antibodies  >6 publications with BUB3 and Brain
 Prostate Antibodies  >3 publications with BUB3 and Prostate
 Thyroid Antibodies  >3 publications with BUB3 and Thyroid
 Liver Antibodies  >3 publications with BUB3 and Liver
 Blood Antibodies  >3 publications with BUB3 and Blood
 Kidney Antibodies  >2 publications with BUB3 and Kidney
 Bone Antibodies  >2 publications with BUB3 and Bone
 Vascular Antibodies  >2 publications with BUB3 and Vascular
 Bladder Antibodies  >2 publications with BUB3 and Bladder
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