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EHD1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1753497

EH domain-containing protein 1, also known as testilin or PAST homolog 1 (PAST1), is a protein that in humans is encoded by the EHD1 gene, belonging to the EHD protein family. It is mapped to 11q13. 1. This gene belongs to a highly conserved gene family… More details

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Product Overview

Online Datasheet in PDF
Product Name
EHD1, Polyclonal Antibody
Full Product Name
Anti-EHD1 Antibody
Product Gene Name
Product Synonym Names
EHD1; PAST; PAST1; CDABP0131; EH domain-containing protein 1; PAST homolog 1; hPAST1; Testilin; EH domain containing 1
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Monkey
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Rabbit IgG polyclonal antibody for EHD1 detection.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
60,627 Da
OMIM
605888
3D Structure
ModBase 3D Structure for Q9H4M9
Immunogen
A synthetic peptide corresponding to a sequence of human EHD1 (HELPADLPPHLVPPSKRRHE).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems||Lab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for ICC.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-EHD1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM)
Application Notes for anti-EHD1 antibody
WB: 0.25-0.5ug/ml|Human, Mouse, Monkey|
ICC/IF: 5ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|

Product References and Citations for anti-EHD1 antibody

1. Haider, N. B., Searby, C., Galperin, E., Mintz, L., Horowitz, M., Stone, E. M., Sheffield, V. C. Evaluation and molecular characterization of EHD1, a candidate gene for Bardet-Biedl syndrome 1 (BBS1). Gene 240: 227-232, 1999.
2. Mintz, L., Galperin, E., Pasmanik-Chor, M., Tulzinsky, S., Bromberg, Y., Kozak, C. A., Joyner, A., Fein, A., Horowitz, M. EHD1--an EH-domain-containing protein with a specific expression pattern. Genomics 59: 66-76, 1999.
3. Naslavsky, N., Boehm, M., Backlund, P. S., Jr., Caplan, S. Rabenosyn-5 and EHD1 interact and sequentially regulate protein recycling to the plasma membrane. Molec. Biol. Cell 15: 2410-2422, 2004.

Other Notes

Small volumes of anti-EHD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EHD1 purchase

MBS1753497 is an antibody targeting EHD1. Reactive in Human, Mouse, Monkey. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EHD1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
EH domain-containing protein 1 isoform 1
NCBI Official Synonym Full Names
EH-domain containing 1
NCBI Official Symbol
EHD1
NCBI Official Synonym Symbols
PAST; PAST1; H-PAST; HPAST1
UniProt Protein Name
EH domain-containing protein 1
UniProt Synonym Protein Names
PAST homolog 1; hPAST1; Testilin
UniProt Gene Name
EHD1
UniProt Synonym Gene Names
PAST; PAST1; hPAST1
UniProt Entry Name
EHD1_HUMAN
NCBI Protein Information
EH domain-containing protein 1; testilin; PAST homolog 1

NCBI Summary for EHD1

This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]
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UniProt Comments for EHD1

EHD1: Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes.

Protein type: Calcium-binding; Vesicle

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: membrane; recycling endosome membrane; early endosome membrane; plasma membrane; lipid particle; endosome membrane

Molecular Function: protein binding; GTP binding; calcium ion binding; ATP binding; Rab GTPase binding

Biological Process: intracellular protein transport; cholesterol homeostasis; endocytosis; blood coagulation; endocytic recycling; neurite development; protein homooligomerization
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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