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POLL Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9214080

This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and… More details

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Product Overview

Online Datasheet in PDF
Product Name
POLL, Polyclonal Antibody
Full Product Name
POLL Antibody (C-term)
Product Gene Name
Product Synonym Names
DNA polymerase lambda; Pol Lambda; 4299-; DNA polymerase beta-2; Pol beta2; DNA polymerase kappa; POLL
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human (Predicted Reactivity: Monkey, Mouse, Rat)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Concentration
Vial Concentration: 2 (lot specific)
Specificity
This POLL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 549-575 amino acids from the C-terminal region of human POLL.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
POLL peptide (MBS9228494) is used for blocking the activity of POLL antibody (MBS9214080)
Molecular Weight
63482
Immunogen Sequence Positions
549-575
OMIM
606343
3D Structure
ModBase 3D Structure for Q9UGP5
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Applications Tested/Suitable for anti-POLL antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-POLL antibody
WB~~1:1000

Western Blot (WB) of anti-POLL antibody

Western blot analysis of POLL Antibody (C-term) in HepG2 cell line lysates (35ug/lane). POLL (arrow) was detected using the purified Pab.
Rabbit Anti-POLL Polyclonal Antibody – Cat# MBS9214080

Product References and Citations for anti-POLL antibody

Brown, J.A., et al. J. Mol. Biol. 395(2):282-290(2010)
van Loon, B., et al. Proc. Natl. Acad. Sci. U.S.A. 106(43):18201-18206(2009)
Garcia-Diaz, M., et al. Nat. Struct. Mol. Biol. 16(9):967-972(2009)

Other Notes

Small volumes of anti-POLL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLL purchase

MBS9214080 is an antibody targeting POLL. Also known as POLL Antibody (C-term). Reactive in Human (Predicted Reactivity: Monkey, Mouse, Rat). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for POLL. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA polymerase lambda isoform a
NCBI Official Synonym Full Names
polymerase (DNA directed), lambda
NCBI Official Symbol
POLL
NCBI Official Synonym Symbols
BETAN; POLKAPPA
UniProt Protein Name
DNA polymerase lambda
UniProt Synonym Protein Names
DNA polymerase beta-2; Pol beta2; DNA polymerase kappa
Protein Family
UniProt Gene Name
POLL
UniProt Synonym Gene Names
Pol Lambda; Pol beta2
UniProt Entry Name
DPOLL_HUMAN
NCBI Protein Information
DNA polymerase lambda

NCBI Summary for POLL

This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010]
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UniProt Comments for POLL

POLL: Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. Belongs to the DNA polymerase type-X family.

Protein type: DNA repair, damage; EC 2.7.7.7; Transferase; Lyase

Chromosomal Location of Human Ortholog: 10q23

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding; DNA-directed DNA polymerase activity; lyase activity

Biological Process: nucleotide-excision repair; somatic hypermutation of immunoglobulin genes; DNA replication
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-POLL antibody

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