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ACTBL2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9606253

MBS9606253 is an antibody targeting ACTBL2. Also known as ACTBL2 Antibody. Reactive in Human, Mouse. Host: Rabbit. More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ACTBL2, Polyclonal Antibody
Full Product Name
ACTBL2 Antibody
Product Gene Name
anti-ACTBL2 antibody
Product Synonym Names
ACT; ACTBL_HUMAN; ACTBL2; actin like protein; actin, beta like 2; Beta-actin-like protein 2; DKFZp686D0972; kappa actin; Kappa-actin
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
~1mg/ml (lot specific)
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
ACTBL2 peptide (MBS9624970) is used for blocking the activity of ACTBL2 antibody (MBS9606253)
Molecular Weight
40 kD
3D Structure
ModBase 3D Structure for Q562R1
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human ACTBL2, corresponding to a region within the internal amino acids
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ACTBL2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Application Notes for anti-ACTBL2 antibody
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IHC: 1:50-1:200

The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk, 1X TBS, 0.1% Tween20 at 4°C with gentle shaking overnight.

Immunofluorescence (IF) of anti-ACTBL2 antibody

MBS9606253 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab (MBS9606253) and mouse anti-beta tubulin Ab (MBS9610328) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).
Rabbit Anti-ACTBL2 Polyclonal Antibody – Cat# MBS9606253

Western Blot (WB) of anti-ACTBL2 antibody

Western blot analysis of extracts from HepG2 using ACTBL2 antibody.
Rabbit Anti-ACTBL2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9606253

Immunohistochemistry (IHC) of anti-ACTBL2 antibody

MBS9606253 at 1/100 staining Rat muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.
Rabbit Anti-ACTBL2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9606253

Other Notes

Small volumes of anti-ACTBL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTBL2. It may not necessarily be applicable to this product.

NCBI GI #
63055057
NCBI GeneID
345651
NCBI Accession #
NP_001017992.1
UniProt Primary Accession #
Q562R1
UniProt Secondary Accession #
Q562R2; Q562S9; Q562X8; B2RPJ1
UniProt Related Accession #
Q562R1
GenBank Nucleotide #
NM_001017992.3
NCBI Official Full Name
beta-actin-like protein 2
NCBI Official Synonym Full Names
actin, beta like 2
NCBI Official Symbol
ACTBL2
NCBI Official Synonym Symbols
ACT
UniProt Protein Name
Beta-actin-like protein 2
UniProt Synonym Protein Names
Kappa-actin
Protein Family
Beta-actin-like protein
UniProt Gene Name
ACTBL2
NCBI Protein Information
beta-actin-like protein 2

UniProt Comments for ACTBL2

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Research Articles on ACTBL2

1. identified a novel protein, ACTBL2, which was demonstrated to be upregulated in colorectal cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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