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ASBT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9603579

MBS9603579 is an antibody targeting ASBT. Also known as ASBT Antibody. Reactive in Human, Mouse. Host: Rabbit. More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ASBT, Polyclonal Antibody
Full Product Name
ASBT Antibody
Product Gene Name
anti-ASBT antibody
Product Synonym Gene Name
SLC10A2
Product Synonym Names
Apical sodium dependent bile acid transporter; Apical sodium-dependent bile acid transporter; Apical sodium-dependent bile acid transporter; ASBT; IBAT; ileal; ileal apical sodium-dependent bile acid transporter; Ileal Na(+)/bile acid cotransporter; Ileal sodium dependent bile acid transporter; Ileal sodium-dependent bile acid transporter; Ileal sodium/bile acid cotransporter; ISBT; Na(+)-dependent ileal bile acid transporter; Na+ bile acid cotransporter; Na+ dependent ileal bile acid transporter; NTCP2; NTCP2_HUMAN; PBAM; SLC10A2; Sodium/taurocholate cotransporting polypeptide; Sodium/taurocholate cotransporting polypeptide, ileal; solute carrier family 10 (sodium/bile acid cotransporter family); Solute carrier family 10 (sodium/bile acid cotransporter family), member 2; Solute carrier family 10 member 2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Concentration
~1mg/ml (lot specific)
Preparation and Storage
Store at -20°C.
Antibody/Peptide Pairs
ASBT peptide (MBS9621056) is used for blocking the activity of ASBT antibody (MBS9603579)
Molecular Weight
38-40 kDa
3D Structure
ModBase 3D Structure for Q12908
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human ASBT, corresponding to a region within C-terminal amino acids.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ASBT antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ASBT antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

The optimal dilutions should be determined by the end user.

IMPORTANT:
For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween20 at 4°C with gentle shaking, overnight.

Western Blot (WB) of anti-ASBT antibody

Western blot analysis of extracts from Mouse brain, using ASBT Ab. The lane on the left was treated with blocking peptide
Rabbit Anti-ASBT Polyclonal Antibody – Cat# MBS9603579

Immunohistochemistry (IHC) of anti-ASBT antibody

MBS9603579 at 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.
Rabbit Anti-ASBT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9603579

Other Notes

Small volumes of anti-ASBT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ASBT. It may not necessarily be applicable to this product.

NCBI GI #
4506973
NCBI GeneID
6555
NCBI Accession #
NP_000443.1
UniProt Primary Accession #
Q12908
UniProt Secondary Accession #
Q13839; A1L4F4
UniProt Related Accession #
Q12908
GenBank Nucleotide #
NM_000452.2
NCBI Official Full Name
ileal sodium/bile acid cotransporter
NCBI Official Synonym Full Names
solute carrier family 10 member 2
NCBI Official Symbol
SLC10A2
NCBI Official Synonym Symbols
ASBT; IBAT; ISBT; PBAM; NTCP2
UniProt Protein Name
Ileal sodium/bile acid cotransporter
UniProt Synonym Protein Names
Apical sodium-dependent bile acid transporter; ASBT; Ileal Na(+)/bile acid cotransporter; Ileal sodium-dependent bile acid transporter; IBAT; ISBT; Na(+)-dependent ileal bile acid transporter; Sodium/taurocholate cotransporting polypeptide, ileal; Solute carrier family 10 member 2
Protein Family
Ileal sodium/bile acid cotransporter
UniProt Gene Name
SLC10A2
UniProt Synonym Gene Names
ASBT; ISBT; NTCP2; ASBT; IBAT; ISBT
NCBI Protein Information
ileal sodium/bile acid cotransporter

NCBI Summary for ASBT

This gene encodes a sodium/bile acid cotransporter. This transporter is the primary mechanism for uptake of intestinal bile acids by apical cells in the distal ileum. Bile acids are the catabolic product of cholesterol metabolism, so this protein is also critical for cholesterol homeostasis. Mutations in this gene cause primary bile acid malabsorption (PBAM); muatations in this gene may also be associated with other diseases of the liver and intestines, such as familial hypertriglyceridemia (FHTG). [provided by RefSeq, Mar 2010]
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UniProt Comments for ASBT

Plays a critical role in the sodium-dependent reabsorption of bile acids from the lumen of the small intestine. Plays a key role in cholesterol metabolism.

Research Articles on ASBT

1. A cysteine-less form of hASBT was made by creating point mutations at all 13 endogenous cysteines. Cysless hASBT had significantly reduced function correlated with lowered surface expression.Chemical cross-linking of wild-type and Cysless species revealed that hASBT has a dominant negative effect and exists as an active dimer and/or higher order oligomer with apparently no requirement for endogenous cysteine residues.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ASBT antibody

 Reference Product  PubMed Publications
 CYP7A1 antibody  >7 publications with ASBT and CYP7A1
 OSTBETA antibody  >5 publications with ASBT and OSTBETA
 ABCB11 antibody  >3 publications with ASBT and ABCB11
 ABCC3 antibody  >3 publications with ASBT and ABCC3
 NR1H4 antibody  >1 publications with ASBT and NR1H4
 FABP6 antibody  >1 publications with ASBT and FABP6

Pathways associated with anti-ASBT antibody

 Products by Pathway  Pathway Diagram
 Bile Acid And Bile Salt Metabolism Pathway antibodies  Bile Acid And Bile Salt Metabolism Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Orphan Transporters Pathway antibodies  Orphan Transporters Pathway Diagram
 Recycling Of Bile Acids And Salts Pathway antibodies  Recycling Of Bile Acids And Salts Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram

Diseases associated with anti-ASBT antibody

 Disease Name  Pubmed Publications
 Cholestasis Antibodies  >7 publications with ASBT and Cholestasis
 Liver Diseases Antibodies  >5 publications with ASBT and Liver Diseases
 Disease Models, Animal Antibodies  >3 publications with ASBT and Disease Models, Animal
 Dyslipidemias Antibodies  >3 publications with ASBT and Dyslipidemias
 Fatty Liver Antibodies  >2 publications with ASBT and Fatty Liver
 Cholestasis, Intrahepatic Antibodies  >2 publications with ASBT and Cholestasis, Intrahepatic
 Cardiovascular Diseases Antibodies  >1 publications with ASBT and Cardiovascular Diseases
 Inflammation Antibodies  >1 publications with ASBT and Inflammation
 Hyperplasia Antibodies  >1 publications with ASBT and Hyperplasia
 Atherosclerosis Antibodies  >1 publications with ASBT and Atherosclerosis

Organs/Tissues associated with anti-ASBT antibody

 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >31 publications with ASBT and Intestine
 Kidney Antibodies  >7 publications with ASBT and Kidney
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