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BRD4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3902412

Bromodomain-containing protein 4 (BRD4) contains 2 bromo-domains. Bromo-domains are found in chromatin-associating proteins and can interact specifically with acetylated lysine. BRD4 is described as a chromatin adaptor and has been shown to bind acetylated… More details

$250 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
BRD4, Polyclonal Antibody
Full Product Name
Rabbit anti-BRD4 Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
HUNK1; Protein HUNK1; HUNKI; chromosome-associated protein; CAP; bromodomain-containing protein 4; MCAP; Bromodomain-containing protein 4; bromodomain containing 4
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
155.8kDa
OMIM
608749
3D Structure
ModBase 3D Structure for O60885
Immunogen
Between 1312 and C-terminus
Conjugation
Unconjugated

Product Categories/Family for anti-BRD4 antibody

Applications Tested/Suitable for anti-BRD4 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-BRD4 antibody
WB: 1:2000-1:10,000
IP: 6ug/mg lysate
IHC: 1:1000-1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-BRD4 antibody

Detection of mouse BRD4 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-BRD4 (Cat. No. MBS3902412 lot 8) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Rabbit Anti-BRD4 Polyclonal Antibody – Cat# MBS3902412

Immunohistochemistry (IHC) of anti-BRD4 antibody

Detection of human BRD4 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD4 (Cat. No. MBS3902412 lot 8) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Rabbit Anti-BRD4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS3902412

Immunoprecipitation (IP) of anti-BRD4 antibody

Detection of human BRD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD4 antibody MBS3902412 (lot MBS3902412-8) used for IP at 6 ug per reaction. BRD4 was also immunoprecipitated by a previous lot of this antibody (lot MBS3902412-7). For blotting immunoprecipitated BRD4, rabbit anti-BRD4 recombinant monoclonal antibody MBS3906216 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.
Rabbit Anti-BRD4 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS3902412

Western Blot (WB) of anti-BRD4 antibody

Detection of mouse BRD4 by western blot. Samples: Whole cell lysate (15 ug) from BW5147.3, EL4, RenCa, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD4 antibody MBS3902412 (lot MBS3902412-8) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Rabbit Anti-BRD4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3902412

Western Blot (WB) of anti-BRD4 antibody

Detection of human BRD4 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, MDA-MB-231, Hep-G2, A-549, SW620, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD4 antibody MBS3902412 (lot MBS3902412-8) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-BRD4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3902412

Other Notes

Small volumes of anti-BRD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BRD4 purchase

MBS3902412 is an antibody targeting BRD4. Also known as Rabbit anti-BRD4 Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BRD4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
bromodomain-containing protein 4 isoform long
NCBI Official Synonym Full Names
bromodomain containing 4
NCBI Official Symbol
BRD4
NCBI Official Synonym Symbols
CAP; MCAP; HUNK1; HUNKI
UniProt Protein Name
Bromodomain-containing protein 4
UniProt Synonym Protein Names
Protein HUNK1
UniProt Gene Name
BRD4
UniProt Synonym Gene Names
HUNK1
UniProt Entry Name
BRD4_HUMAN
NCBI Protein Information
bromodomain-containing protein 4

NCBI Summary for BRD4

The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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