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CD73 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6283452

MBS6283452 is an antibody targeting CD73. Also known as CD73 (NT5E), CT (NT5E, NT5, NTE, 5'-nucleotidase, Ecto-5'-nucleotidase, CD73) (MaxLight 550). Reactive in Human, Mouse. Host: Rabbit. More details

$1060 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD73, Polyclonal Antibody
Full Product Name
CD73 (NT5E), CT (NT5E, NT5, NTE, 5'-nucleotidase, Ecto-5'-nucleotidase, CD73) (MaxLight 550)
Product Gene Name
anti-CD73 antibody
Product Synonym Gene Name
NT5E
Product Synonym Names
NT5E; NT5; NTE; 5'-nucleotidase; Ecto-5'-nucleotidase; CD73
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human CD73. Species Crossreactivity: mouse
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
57,949 Da
OMIM
gene 211800
3D Structure
ModBase 3D Structure for P21589
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 520-550 in the C-terminal region of human CD73, UniProt Accession #P21589.
Conjugate
MaxLight550
Note
Preservative Free

Product Categories/Family for anti-CD73 antibody

Antibodies; CD Markers
Applications Tested/Suitable for anti-CD73 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), FLISA
Application Notes for anti-CD73 antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-CD73 antibody

Western Blot analysis of 033537 in mouse brain tissue lysate. CD73 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Rabbit Anti-CD73 Polyclonal Antibody – Cat# MBS6283452

Western Blot (WB) of anti-CD73 antibody

Western Blot analysis of NT5E (arrow) using 033537. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NT5E gene.
Rabbit Anti-CD73 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6283452

Testing Data of anti-CD73 antibody

All lanes: 033537 (1:1000) Lane 1: HepG2 whole cell lysate Lane 2: U266B1 whole cell lysate. Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 63kD Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-CD73 Polyclonal Antibody, Testing Data – Cat# MBS6283452

Western Blot (WB) of anti-CD73 antibody

Western Blot analysis of lysates from U266B1, HepG2 cell line, mouse brain tissue lysate (from left to right at 20ug/lane), using 033537 (1:2000/lane). A goat anti-rabbit IgG H&L(HRP) at 1:10,000 dilution was used as the secondary antibody.
Rabbit Anti-CD73 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6283452

Immunohistochemistry (IHC) of anti-CD73 antibody

Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human Placenta tissue reacted with 033537, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Rabbit Anti-CD73 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6283452

Product References and Citations for anti-CD73 antibody

1. Hashikawa, T., et al., J. Dent. Res. 82(11):888-892 (2003). 2. Rosi, F., et al., Biomed. Pharmacother. 56(2):100-104 (2002). 3. Misumi, Y., et al., Eur. J. Biochem. 191(3):563-569 (1990). 4. Boyle, J.M., et al., Hum. Genet. 81(1):88-92 (1988). 5. Kalsi, K., et al., Mol. Cell. Biochem. 232 (1-2), 113-119 (2002).

Other Notes

Small volumes of anti-CD73 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD73. It may not necessarily be applicable to this product.

NCBI GI #
4505467
NCBI GeneID
4907
NCBI Accession #
NP_002517
UniProt Primary Accession #
P21589
UniProt Secondary Accession #
O75520; Q5W116; B3KQI8
UniProt Related Accession #
P21589
GenBank Nucleotide #
NM_002526.3
NCBI Official Full Name
5'-nucleotidase isoform 1 preproprotein
NCBI Official Synonym Full Names
5'-nucleotidase ecto
NCBI Official Symbol
NT5E
NCBI Official Synonym Symbols
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
UniProt Protein Name
5'-nucleotidase
UniProt Synonym Protein Names
Ecto-5'-nucleotidase; CD_antigen: CD73
UniProt Gene Name
NT5E
UniProt Synonym Gene Names
NT5; NTE; 5'-NT
UniProt Entry Name
5NTD_HUMAN
NCBI Protein Information
5'-nucleotidase

NCBI Summary for CD73

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
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UniProt Comments for CD73

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.5; Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; Phosphatase (non-protein); Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; dephosphorylation; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries
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Research Articles on CD73

1. Data show that MiR-422a and its target CD73 are involved in early loco(regional) recurrence of head and neck squamous cell carcinoma (HNSCC) tumors and are targets for personalized medicine.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD73 antibody

 Reference Product  PubMed Publications
 ENPP1 antibody  >5 publications with CD73 and ENPP1
 ADA antibody  >3 publications with CD73 and ADA
 ENTPD1 antibody  >3 publications with CD73 and ENTPD1

Pathways associated with anti-CD73 antibody

 Products by Pathway  Pathway Diagram
 Differentiation Pathway antibodies  Differentiation Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Metabolism Of Vitamins And Cofactors Pathway antibodies  Metabolism Of Vitamins And Cofactors Pathway Diagram
 Metabolism Of Water-soluble Vitamins And Cofactors Pathway antibodies  Metabolism Of Water-soluble Vitamins And Cofactors Pathway Diagram
 NAD+ Metabolism Pathway antibodies  NAD+ Metabolism Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram

Diseases associated with anti-CD73 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >17 publications with CD73 and Inflammation
 Necrosis Antibodies  >9 publications with CD73 and Necrosis
 Breast Neoplasms Antibodies  >9 publications with CD73 and Breast Neoplasms
 Nervous System Diseases Antibodies  >9 publications with CD73 and Nervous System Diseases
 Disease Models, Animal Antibodies  >8 publications with CD73 and Disease Models, Animal
 Pain Antibodies  >7 publications with CD73 and Pain
 Brain Diseases Antibodies  >5 publications with CD73 and Brain Diseases
 Kidney Diseases Antibodies  >4 publications with CD73 and Kidney Diseases
 Heart Diseases Antibodies  >4 publications with CD73 and Heart Diseases
 Acute Kidney Injury Antibodies  >3 publications with CD73 and Acute Kidney Injury

Organs/Tissues associated with anti-CD73 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >34 publications with CD73 and Blood
 Vascular Antibodies  >20 publications with CD73 and Vascular
 Bone Antibodies  >14 publications with CD73 and Bone
 Bone Marrow Antibodies  >9 publications with CD73 and Bone Marrow
 Brain Antibodies  >9 publications with CD73 and Brain
 Muscle Antibodies  >8 publications with CD73 and Muscle
 Mouth Antibodies  >8 publications with CD73 and Mouth
 Kidney Antibodies  >6 publications with CD73 and Kidney
 Ganglia Antibodies  >6 publications with CD73 and Ganglia
 Embryonic Tissue Antibodies  >5 publications with CD73 and Embryonic Tissue
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