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CPSF6 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8102144

MBS8102144 is an antibody targeting CPSF6. Also known as Anti-CPSF6 Antibody, Rabbit Polyclonal. Reactive in HumanPredicted to React with: Mouse (Species predicted to react based on 100% sequence homology). Host: Rabbit. More details

$370 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CPSF6, Polyclonal Antibody
Full Product Name
Anti-CPSF6 Antibody, Rabbit Polyclonal
Product Gene Name
Product Synonym Names
CPSF6 Antibody, Rabbit PAb, Antigen Affinity Purified; cleavage and polyadenylation specific factor 6; Anti-CFIM Antibody; Anti-CFIM68 Antibody; Anti-HPBRII-4 Antibody; Anti-HPBRII-7 Antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Form/Format
Liquid; 0.2um filtered solution in PBS
Purity/Purification
Protein A & Antigen Affinity
Specificity
Human CPSF6
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
OMIM
604979
3D Structure
ModBase 3D Structure for Q16630
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human CPSF6
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human CPSF6, and purified by antigen affinity chromatography.
Applications Tested/Suitable for anti-CPSF6 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-CPSF6 antibody
WB: 1:500-1:2000
IHC-P: 1:500-1:2000
ICC/IF: 1:300-1:10,000
IP: 1-4uL/mg of lysate

Western Blot (WB) of anti-CPSF6 antibody

Anti-CPSF6 rabbit polyclonal antibody at 1:500 dilution
Lane A: Jurkat Whole Cell Lysate
Lane B: K562 Whole Cell Lysate
Lane C: A431 Whole Cell Lysate
Lane D: HepG2 Whole Cell Lysate

Lysates/proteins at 30 ug per lane.
Secondary
Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.

Developed using the Odyssey technique.
Performed under reducing conditions.

Predicted band size:59 kDa
Observed band size:72 kDa
(We are unsure as to the identity of these extra bands.)
Rabbit Anti-CPSF6 Polyclonal Antibody – Cat# MBS8102144

Immunohistochemistry (IHC) of anti-CPSF6 antibody

Immunochemical staining of human CPSF6 in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Rabbit Anti-CPSF6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS8102144

Immunohistochemistry (IHC) of anti-CPSF6 antibody

Immunochemical staining of human CPSF6 in human colon carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Rabbit Anti-CPSF6 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS8102144

Immunofluorescence (IF) of anti-CPSF6 antibody

Immunofluorescence staining of CPSF6 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human CPSF6 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.
Rabbit Anti-CPSF6 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS8102144

Immunoprecipitation (IP) of anti-CPSF6 antibody

CPSF6 was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate
Lane B:0.5 mg K562 Whole Cell Lysate
Lane C:0.5 mg Jurkat Whole Cell Lysate

4 uL anti-CPSF6 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.

Primary antibody:
Anti-CPSF6 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.
Performed under reducing conditions.

Predicted band size: 60 kDa
Observed band size: 72 kDa
Rabbit Anti-CPSF6 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS8102144

Other Notes

Small volumes of anti-CPSF6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CPSF6. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
GenBank Nucleotide #
NCBI Official Full Name
cleavage and polyadenylation specificity factor subunit 6 isoform 1
UniProt Protein Name
Cleavage and polyadenylation specificity factor subunit 6
UniProt Synonym Protein Names
Cleavage and polyadenylation specificity factor 68 kDa subunit; CFIm68; CPSF 68 kDa subunit; Pre-mRNA cleavage factor Im 68 kDa subunit; Protein HPBRII-4/7
UniProt Gene Name
CPSF6
UniProt Synonym Gene Names
CFIM68
UniProt Entry Name
CPSF6_HUMAN

NCBI Summary for CPSF6

The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins. [provided by RefSeq, Jul 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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