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MAP3K12 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS630604

ZPK is a member of serine/threonine protein kinase family. This kinase contains a leucine-zipper domain, and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane… More details

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Product Overview

Online Datasheet in PDF
Product Name
MAP3K12, Polyclonal Antibody
Full Product Name
MAP3K12, CT (Mitogen-activated Protein Kinase Kinase Kinase 12, Dual Leucine Zipper Bearing Kinase, DLK, Leucine-zipper Protein Kinase, MAPK-upstream Kinase, MUK, MEKK12, Mixed Lineage Kinase, ZPK, ZPKP1)
Product Gene Name
anti-MAP3K12 antibody
Product Synonym Names
Anti -MAP3K12, CT (Mitogen-activated Protein Kinase Kinase Kinase 12, Dual Leucine Zipper Bearing Kinase, DLK, Leucine-zipper Protein Kinase, MAPK-upstream Kinase, MUK, MEKK12, Mixed Lineage Kinase, ZPK, ZPKP1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human ZPK. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
93,219 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (53873456..53893444, complement). Location: 12q13
OMIM
600447
3D Structure
ModBase 3D Structure for Q12852
Immunogen
Synthetic peptide selected from the C-terminal region of human MAP3K12 (KLH).

Product Categories/Family for anti-MAP3K12 antibody

Antibodies; Abs to Protein Kinases
Applications Tested/Suitable for anti-MAP3K12 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-MAP3K12 antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100

Western Blot (WB) of anti-MAP3K12 antibody

Western blot analysis of anti-ZPK C-term Pab in HL-60 cell lysate (lane A) and in mouse kidney tissue lysate (lane B). ZPK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Rabbit Anti-MAP3K12 Polyclonal Antibody – Cat# MBS630604

Immunohistochemistry (IHC) of anti-MAP3K12 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-MAP3K12 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS630604

Other Notes

Small volumes of anti-MAP3K12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAP3K12 purchase

MBS630604 is an antibody targeting MAP3K12. Also known as MAP3K12, CT (Mitogen-activated Protein Kinase Kinase Kinase 12, Dual Leucine Zipper Bearing Kinase, DLK, Leucine-zipper Protein Kinase, MAPK-upstream Kinase, MUK, MEKK12, Mixed Lineage Kinase, ZPK, ZPKP1). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MAP3K12. It may not necessarily be applicable to this product.

NCBI GI #
29476775
NCBI GeneID
7786
NCBI Accession #
AAH50050.1
UniProt Primary Accession #
Q12852
UniProt Secondary Accession #
Q86VQ5; Q8WY25; B3KSS9; G3V1Y2
UniProt Related Accession #
Q12852
NCBI Official Full Name
MAP3K12 protein
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 12
NCBI Official Symbol
MAP3K12
NCBI Official Synonym Symbols
DLK; MUK; ZPK; ZPKP1; MEKK12
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 12
UniProt Synonym Protein Names
Dual leucine zipper bearing kinase; DLK; Leucine-zipper protein kinase; ZPK; MAPK-upstream kinase; MUK; Mixed lineage kinase
Protein Family
Mitogen-activated protein kinase kinase kinase
UniProt Gene Name
MAP3K12
UniProt Synonym Gene Names
ZPK; DLK; ZPK; MUK
UniProt Entry Name
M3K12_HUMAN
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 12; protein kinase MUK; MAPK-upstream kinase; mixed lineage kinase; leucine zipper protein kinase; dual leucine zipper kinase DLK; dual leucine zipper bearing kinase

NCBI Summary for MAP3K12

This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described.[provided by RefSeq, Jul 2010]
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UniProt Comments for MAP3K12

DLK: May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.25; Protein kinase, TKL; Kinase, protein; TKL group; MLK family; LZK subfamily

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: growth cone; membrane; axon; cytoplasm; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; protein homodimerization activity; MAP kinase kinase kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: peptidyl-serine phosphorylation; activation of MAPKK activity; protein amino acid autophosphorylation; histone phosphorylation; peptidyl-threonine phosphorylation; JNK cascade; protein amino acid phosphorylation
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Research Articles on MAP3K12

1. these findings indicate that DLK participates in cell proliferation and/or survival, at least in part, by modulating the expression of cell cycle regulatory proteins.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAP3K12 antibody

 Reference Product  PubMed Publications
 MBIP antibody  >1 publications with MAP3K12 and MBIP

Pathways associated with anti-MAP3K12 antibody

 Products by Pathway  Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 MAPK Cascade Pathway antibodies  MAPK Cascade Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Regulation Of P38-alpha And P38-beta Pathway antibodies  Regulation Of P38-alpha And P38-beta Pathway Diagram

Diseases associated with anti-MAP3K12 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >3 publications with MAP3K12 and Neoplasms

Organs/Tissues associated with anti-MAP3K12 antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >2 publications with MAP3K12 and Nerve
 Brain Antibodies  >2 publications with MAP3K12 and Brain
 Bone Antibodies  >1 publications with MAP3K12 and Bone
 Bone Marrow Antibodies  >1 publications with MAP3K12 and Bone Marrow
 Blood Antibodies  >1 publications with MAP3K12 and Blood
 Kidney Antibodies  >1 publications with MAP3K12 and Kidney
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