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ATG16L1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS616231

MBS616231 is an antibody targeting ATG16L1. Also known as Atg16L1 (Autophagy-related Protein 16-1, ATG16 Autophagy Related 16-like 1, APG16-like 1, APG16 Autophagy 16-like 1). Reactive in Human, Rat. Host: Rabbit. More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Atg16L1, Polyclonal Antibody
Full Product Name
Atg16L1 (Autophagy-related Protein 16-1, ATG16 Autophagy Related 16-like 1, APG16-like 1, APG16 Autophagy 16-like 1)
Product Gene Name
anti-ATG16L1 antibody
Product Synonym Names
Anti -Atg16L1 (Autophagy-related Protein 16-1, ATG16 Autophagy Related 16-like 1, APG16-like 1, APG16 Autophagy 16-like 1)
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
Purity/Purification
Serum
Serum
Specificity
Recognizes human ATG16 L1. Peptide Sequence Homology: mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
68,265 Da
Chromosome Location
Chromosome: 2; NC_000002.11 (234160217..234204320). Location: 2q37.1
OMIM
610767
3D Structure
ModBase 3D Structure for Q676U5
Immunogen
Synthetic peptide corresponding to the N-terminal of human ATG16-L1 protein conjugated to Blue Carrier Protein.
Cellular Localization
Cytoplasm. Preautophagosomal structure; preautophagosomal structure membrane; peripheral membrane protein. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5.

Product Categories/Family for anti-ATG16L1 antibody

Antibodies; Abs to Autophagy
Applications Tested/Suitable for anti-ATG16L1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-ATG16L1 antibody
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Dilution: Immunofluorescence: 1:100-1:3000
Immunohistochemistry: 1:100-1:3000
Western Blot: 1:100-1:3000

Immunofluorescence (IF) of anti-ATG16L1 antibody

Confocal microscopy on immunofluorescently detected cytospinned-HL60 cell line. HL60 cells were fixed with acetone 90 seconds; primary antibody was diluted 1:100 and incubated for 1 hour, 3 x washes and secondary (Goat anti Rb 1:100 alexa 546 conjugate) for 30 minutes. Eosinophils seem to be stained using MBS616231
Rabbit Anti-ATG16L1 Polyclonal Antibody – Cat# MBS616231

Western Blot (WB) of anti-ATG16L1 antibody

Western blot (non-reducing) on Jurkat cell lysate using MBS616231
Rabbit Anti-ATG16L1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS616231

Western Blot (WB) of anti-ATG16L1 antibody

Western blot on Peripheral Blood Mononuclear Cells (PMBC) lysate using MBS616231 at a dilution of 1: 100 (ECL).
Rabbit Anti-ATG16L1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS616231

Other Notes

Small volumes of anti-ATG16L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG16L1. It may not necessarily be applicable to this product.

NCBI GI #
124256480
NCBI GeneID
55054
NCBI Accession #
NP_110430.5
UniProt Primary Accession #
Q676U5
UniProt Secondary Accession #
Q6IPN1; Q6UXW4; Q6ZVZ5; Q8NCY2; Q96JV5; Q9H619; A3EXK9
UniProt Related Accession #
Q53SV2; Q676U5; Q8TCI4; Q9H7M5; Q9NWG8
GenBank Nucleotide #
NM_030803.6
NCBI Official Full Name
autophagy-related protein 16-1 isoform 1
NCBI Official Synonym Full Names
ATG16 autophagy related 16-like 1 (S. cerevisiae)
NCBI Official Symbol
ATG16L1
NCBI Official Synonym Symbols
IBD10; WDR30; APG16L; ATG16A; ATG16L; FLJ00045; FLJ10035; FLJ10828; FLJ22677
UniProt Protein Name
Autophagy-related protein 16-1
UniProt Synonym Protein Names
APG16-like 1
Protein Family
Autophagy-related protein
UniProt Gene Name
ATG16L1
UniProt Synonym Gene Names
APG16L
UniProt Entry Name
A16L1_HUMAN
NCBI Protein Information
autophagy-related protein 16-1; APG16L beta; OTTHUMP00000164391; OTTHUMP00000164393; OTTHUMP00000165876; OTTHUMP00000165877; WD repeat domain 30

NCBI Summary for ATG16L1

The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.
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UniProt Comments for ATG16L1

ATG16: Plays an essential role in autophagy. Homooligomer. Interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Belongs to the WD repeat ATG16 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Membrane protein, peripheral; Autophagy

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: pre-autophagosomal structure membrane; autophagic vacuole; axoneme

Molecular Function: identical protein binding; protein binding

Biological Process: protein transport; protein homooligomerization; autophagic vacuole formation

Disease: Inflammatory Bowel Disease 10
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Research Articles on ATG16L1

1. IL23R, NOD2/CARD15, ATG16L1 and PHOX2B polymorphisms in a group of patients with Crohn's disease and correlation with sub-phenotypes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ATG16L1 antibody

 Reference Product  PubMed Publications
 ATG5 antibody  >10 publications with ATG16L1 and ATG5

Diseases associated with anti-ATG16L1 antibody

 Disease Name  Pubmed Publications
 Inflammatory Bowel Diseases Antibodies  >114 publications with ATG16L1 and Inflammatory Bowel Diseases
 Crohn Disease Antibodies  >87 publications with ATG16L1 and Crohn Disease
 Necrosis Antibodies  >8 publications with ATG16L1 and Necrosis
 Neurodegenerative Diseases Antibodies  >2 publications with ATG16L1 and Neurodegenerative Diseases
 Liver Diseases Antibodies  >1 publications with ATG16L1 and Liver Diseases
 Gastritis Antibodies  >1 publications with ATG16L1 and Gastritis

Organs/Tissues associated with anti-ATG16L1 antibody

 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >19 publications with ATG16L1 and Intestine
 Blood Antibodies  >7 publications with ATG16L1 and Blood
 Nerve Antibodies  >3 publications with ATG16L1 and Nerve
 Brain Antibodies  >3 publications with ATG16L1 and Brain
 Heart Antibodies  >2 publications with ATG16L1 and Heart
 Muscle Antibodies  >1 publications with ATG16L1 and Muscle
 Liver Antibodies  >1 publications with ATG16L1 and Liver
 Thymus Antibodies  >1 publications with ATG16L1 and Thymus
 Skin Antibodies  >1 publications with ATG16L1 and Skin
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