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KARS Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS9133792
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Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
KARS, Polyclonal Antibody
Full Product Name
KARS Polyclonal Antibody
Product Gene Name
Product Synonym Names
CMTRIB; DFNB89; KARS1; KARS2; KRS
Immunogen Sequence
IAPELYHKML VVGGIDRVYE IGRQFRNEGI DLTHNPEFTT CEFYMAYADY HDLMEITEKM VSGMVKHITG SYKVTYHPDG PEGQAYDVDF TPPFRRINMV EELEKALGMK LPETNLFETE ETRKILDDIC VAKAVECPPP RTTARLLDKL VGEFLEVTCI NPTFICDHPQ IMSPLAKWHR SKEGLTERFE LFVMKKEICN AYTELNDPMR QRQLFEEQAK AKAAGDDEAM FIDENFCTAL EYGLPPTAGW GMGID
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Molecular Weight
Calculated: 68kDa; 71kDa
Observed: 75kDa
OMIM
D32053 mRNA
3D Structure
ModBase 3D Structure for Q15046
Immunogen
Recombinant protein of human KARS
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Cell membrane, Cytoplasm, Mitochondrion, Nucleus, Peripheral membrane protein, Secreted
Positive Samples
HepG2, Jurkat, HeLa, 293T, Mouse heart, Mouse kidney, Rat liver

Product Categories/Family for anti-KARS antibody

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-KARS antibody
Western Blot (WB)
Application Notes for anti-KARS antibody
WB: 1:500 - 1:2000

Western Blot (WB) of anti-KARS antibody

Western blot analysis of extracts of various cell lines, using KARS antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Rabbit Anti-KARS Polyclonal Antibody – Cat# MBS9133792

Other Notes

Small volumes of anti-KARS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KARS purchase

MBS9133792 is an antibody targeting KARS. Also known as KARS Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for KARS. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
lysine--tRNA ligase isoform 1
NCBI Official Synonym Full Names
lysyl-tRNA synthetase
NCBI Official Symbol
KARS
NCBI Official Synonym Symbols
KRS; KARS1; KARS2; CMTRIB; DFNB89
UniProt Protein Name
Lysine--tRNA ligase
UniProt Synonym Protein Names
Lysyl-tRNA synthetase; LysRS
UniProt Gene Name
KARS
UniProt Synonym Gene Names
KIAA0070; LysRS
NCBI Protein Information
lysine--tRNA ligase

UniProt Comments for KARS

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:9278442, PubMed:18029264, PubMed:18272479). When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed:15851690). Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed:5338216, PubMed:14975237, PubMed:19524539, PubMed:23159739).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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