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KI67 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8304273

MBS8304273 is an antibody targeting Ki67. Reactive in Human, Mouse, Rat. Host: Rabbit. Rabbit polyclonal antibody to Ki67 More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ki67, Polyclonal Antibody
Full Product Name
Anti-Ki67 Antibody
Product Gene Name
anti-Ki67 antibody
Product Synonym Gene Name
MKI67
Product Synonym Names
Antigen KI-67
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Purity/Purification
Purified by immunogen affinity chromatography.
Specificity
Recognizes endogenous levels of Ki67 protein.
Preparation and Storage
Ship on blue ice.
Upon delivery aliquot and store at -20°C for up to one year.
Avoid freeze/thaw cycles.
3D Structure
ModBase 3D Structure for P46013
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within human Ki67. The exact sequence is proprietary.
Gene Symbol
MKI67
Applications Tested/Suitable for anti-Ki67 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-Ki67 antibody
WB: 1:500 - 1:1000
IHC: 1:50 - 1:200
IF/ICC: 1:50 - 1:200

Western Blot (WB) of anti-Ki67 antibody

Western blot analysis of Ki67 expression in HepG2 (A), Raji (B) whole cell lysates.
Rabbit Anti-KI67 Polyclonal Antibody – Cat# MBS8304273

Immunohistochemistry (IHC) of anti-Ki67 antibody

Immunohistochemical analysis of Ki67 staining in human spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-KI67 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS8304273

Immunofluorescence (IF) of anti-Ki67 antibody

Immunofluorescent analysis of Ki67 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Rabbit Anti-KI67 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS8304273

Other Notes

Small volumes of anti-Ki67 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Ki67. It may not necessarily be applicable to this product.

NCBI GI #
225543215
NCBI GeneID
4288
NCBI Accession #
NP_001139438.1
UniProt Primary Accession #
P46013
UniProt Secondary Accession #
Q5VWH2
UniProt Related Accession #
P46013
GenBank Nucleotide #
NM_001145966.1
NCBI Official Full Name
proliferation marker protein Ki-67 isoform 2
NCBI Official Synonym Full Names
marker of proliferation Ki-67
NCBI Official Symbol
MKI67
NCBI Official Synonym Symbols
KIA; MIB-; MIB-1; PPP1R105
UniProt Protein Name
Proliferation marker protein Ki-67
UniProt Synonym Protein Names
Antigen identified by monoclonal antibody Ki-671 PublicationManual assertion based on opinion iniRef.1"The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins."Schlueter C., Duchrow M., Wohlenberg C., Becker M.H.G., Key G., Flad H.-D., Gerdes J.J. Cell Biol. 123:513-522(1993) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS SHORT AND LONG), VARIANTS VAL-872; TRP-1470; LEU-1622; ALA-1849 AND ASP-3097.
Protein Family
Proliferation marker protein
UniProt Gene Name
MKI67
UniProt Synonym Gene Names
Antigen KI-67Curated; Antigen Ki67Curated
NCBI Protein Information
proliferation marker protein Ki-67

NCBI Summary for Ki67

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

UniProt Comments for Ki67

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
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Research Articles on Ki67

1. Prognosis of luminal breast carcinoma can be predicted using Ki67 as a continuous variable and a standard cut-off value of 14%. Information about the specimen type used to determine ki67 should be recorded in the pathological report

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Ki67 antibody

 Reference Product  PubMed Publications
 MKI67IP antibody  >8 publications with Ki67 and MKI67IP
 CBX3 antibody  >1 publications with Ki67 and CBX3

Diseases associated with anti-Ki67 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >9254 publications with Ki67 and Neoplasms
 Adenocarcinoma Antibodies  >2117 publications with Ki67 and Adenocarcinoma
 Breast Neoplasms Antibodies  >1548 publications with Ki67 and Breast Neoplasms
 Nervous System Diseases Antibodies  >1433 publications with Ki67 and Nervous System Diseases
 Neoplasm Metastasis Antibodies  >1044 publications with Ki67 and Neoplasm Metastasis
 Carcinoma, Squamous Cell Antibodies  >981 publications with Ki67 and Carcinoma, Squamous Cell
 Adenoma Antibodies  >693 publications with Ki67 and Adenoma
 Hyperplasia Antibodies  >583 publications with Ki67 and Hyperplasia
 Colorectal Neoplasms Antibodies  >581 publications with Ki67 and Colorectal Neoplasms
 Neoplasms, Experimental Antibodies  >534 publications with Ki67 and Neoplasms, Experimental

Organs/Tissues associated with anti-Ki67 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1742 publications with Ki67 and Blood
 Brain Antibodies  >1239 publications with Ki67 and Brain
 Vascular Antibodies  >1110 publications with Ki67 and Vascular
 Lymph Node Antibodies  >1097 publications with Ki67 and Lymph Node
 Lymph Antibodies  >1092 publications with Ki67 and Lymph
 Skin Antibodies  >869 publications with Ki67 and Skin
 Lung Antibodies  >635 publications with Ki67 and Lung
 Liver Antibodies  >604 publications with Ki67 and Liver
 Muscle Antibodies  >596 publications with Ki67 and Muscle
 Bone Antibodies  >523 publications with Ki67 and Bone
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