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MCM3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6404525

MBS6404525 is an antibody targeting MCM3. Also known as MCM3 (DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3, RLF subunit beta, p102) (Biotin). Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$825 / 0.1 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MCM3, Polyclonal Antibody
Full Product Name
MCM3 (DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3, RLF subunit beta, p102) (Biotin)
Product Gene Name
anti-MCM3 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Purity/Purification
Purified by Immunoaffinity chromatography.
Specificity
Recognizes human MCM3. No crossreactivity with other proteins. Species Crossreactivity: mouse and rat
Preparation and Storage
Store at -20 degree C.
Molecular Weight
808
OMIM
602693
3D Structure
ModBase 3D Structure for P25205
Immunogen
A synthetic peptide corresponding to aa13-27, REAQRDYLDFLDDEE at the N-terminal of human MCM3, identical to the related rat and mouse sequences.
Conjugate
Biotin
Note
Preservative Free

Product Categories/Family for anti-MCM3 antibody

Antibodies; Cell Division Cycle (CDC)
Applications Tested/Suitable for anti-MCM3 antibody
Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-MCM3 antibody
ICC: 0.5-1ug/ml.
WB: 0.1-0.5ug/ml
IHC-P: 0.5-1ug/ml. Antigen retrieval by boiling paraffin sections in 10mM citrate buffer, pH 6.0 for 20 minutes is required for the staining of paraffin sections.
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MCM3 antibody

WB analysis using antibody 144886: Lane 1: HeLa cell lysate Lane 2: JURKAT cell lysate
Rabbit Anti-MCM3 Polyclonal Antibody – Cat# MBS6404525

Immunohistochemistry (IHC) of anti-MCM3 antibody

IHC staining of paraffin-embedded human lung cancer tissue using antibody 144886.
Rabbit Anti-MCM3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6404525

Immunohistochemistry (IHC) of anti-MCM3 antibody

IHC staining of paraffin-embedded rat intestine tissue using antibody 144886.
Rabbit Anti-MCM3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6404525

Testing Data of anti-MCM3 antibody

IC staining of HeLa cells using antibody 144886.
Rabbit Anti-MCM3 Polyclonal Antibody, Testing Data – Cat# MBS6404525

Product References and Citations for anti-MCM3 antibody

1. Groth, A., Corpet, A., Cook, A. J. L., Roche, D., Bartek, J., Lukas, J., Almouzni, G. Regulation of replication fork progression through histone supply and demand. Science 318: 1928-1931, 2007. 2. Thommes P, Fett R, Schray B, Burkhart R, Barnes M, Kennedy C, Brown NC, Knippers R (Apr 1992). "Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein". Nucleic Acids Res 20 (5): 1069-74. 3. "Entrez Gene: MCM3 MCM3 minichromosome maintenance deficient 3 (S. cerevisiae).

Other Notes

Small volumes of anti-MCM3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MCM3. It may not necessarily be applicable to this product.

NCBI GI #
19857543
NCBI GeneID
4172
NCBI Accession #
P25205.3
UniProt Primary Accession #
P25205
UniProt Secondary Accession #
Q92660; Q9BTR3; Q9NUE7; B4DWW4
UniProt Related Accession #
P25205
NCBI Official Full Name
DNA replication licensing factor MCM3
NCBI Official Synonym Full Names
minichromosome maintenance complex component 3
NCBI Official Symbol
MCM3
NCBI Official Synonym Symbols
HCC5; P1.h; RLFB; P1-MCM3
UniProt Protein Name
DNA replication licensing factor MCM3
UniProt Synonym Protein Names
DNA polymerase alpha holoenzyme-associated protein P1; P1-MCM3; RLF subunit beta; p102
Protein Family
DNA replication licensing factor
UniProt Gene Name
MCM3
UniProt Entry Name
MCM3_HUMAN
NCBI Protein Information
DNA replication licensing factor MCM3; p102; RLF subunit beta; hRlf beta subunit; DNA replication factor MCM3; cervical cancer proto-oncogene 5; minichromosome maintenance deficient 3; replication licensing factor, beta subunit; MCM3 minichromosome mainte

NCBI Summary for MCM3

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
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UniProt Comments for MCM3

MCM3: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for DNA replication and cell proliferation.

Protein type: EC 3.6.4.12; DNA replication

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: nucleoplasm; centrosome; alpha DNA polymerase:primase complex; membrane; intracellular membrane-bound organelle; MCM complex; perinuclear region of cytoplasm; nucleus

Molecular Function: DNA helicase activity; protein binding; DNA binding; ATP binding

Biological Process: DNA replication initiation; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication; DNA duplex unwinding; G1/S transition of mitotic cell cycle
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Research Articles on MCM3

1. The relatively lower MCM3 protein expression in FVPTC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MCM3 antibody

 Reference Product  PubMed Publications
 MCM2 antibody  >91 publications with MCM3 and MCM2
 MCM7 antibody  >51 publications with MCM3 and MCM7
 MCM4 antibody  >48 publications with MCM3 and MCM4
 MCM5 antibody  >44 publications with MCM3 and MCM5
 MCM6 antibody  >36 publications with MCM3 and MCM6
 CDC6 antibody  >23 publications with MCM3 and CDC6
 CDC45 antibody  >13 publications with MCM3 and CDC45
 CDK2 antibody  >10 publications with MCM3 and CDK2

Pathways associated with anti-MCM3 antibody

 Products by Pathway  Pathway Diagram
 Activation Of ATR In Response To Replication Stress Pathway antibodies  Activation Of ATR In Response To Replication Stress Pathway Diagram
 Activation Of The Pre-replicative Complex Pathway antibodies  Activation Of The Pre-replicative Complex Pathway Diagram
 Assembly Of The Pre-replicative Complex Pathway antibodies  Assembly Of The Pre-replicative Complex Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 DNA Replication Pathway antibodies  DNA Replication Pathway Diagram

Diseases associated with anti-MCM3 antibody

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >8 publications with MCM3 and Breast Neoplasms
 Adenocarcinoma Antibodies  >8 publications with MCM3 and Adenocarcinoma
 Nervous System Diseases Antibodies  >7 publications with MCM3 and Nervous System Diseases
 Skin Neoplasms Antibodies  >7 publications with MCM3 and Skin Neoplasms
 Neoplasms, Experimental Antibodies  >4 publications with MCM3 and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >4 publications with MCM3 and Cell Transformation, Neoplastic
 Liver Neoplasms Antibodies  >4 publications with MCM3 and Liver Neoplasms
 Liver Diseases Antibodies  >4 publications with MCM3 and Liver Diseases
 Precancerous Conditions Antibodies  >3 publications with MCM3 and Precancerous Conditions
 Kidney Diseases Antibodies  >3 publications with MCM3 and Kidney Diseases

Organs/Tissues associated with anti-MCM3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >12 publications with MCM3 and Blood
 Skin Antibodies  >9 publications with MCM3 and Skin
 Lymph Node Antibodies  >9 publications with MCM3 and Lymph Node
 Liver Antibodies  >7 publications with MCM3 and Liver
 Kidney Antibodies  >7 publications with MCM3 and Kidney
 Brain Antibodies  >6 publications with MCM3 and Brain
 Lymph Antibodies  >5 publications with MCM3 and Lymph
 Stomach Antibodies  >5 publications with MCM3 and Stomach
 Lung Antibodies  >5 publications with MCM3 and Lung
 Intestine Antibodies  >4 publications with MCM3 and Intestine
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