• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

NAA20 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS9125312
SPECIAL PROMOTION: Get FREE $5 Amazon gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

NAT5 is a component of N-acetyltransferase complex B (NatB). Human NatB performs cotranslational N(alpha)-terminal acetylation of methionine residues when they are followed by asparagine (Starheim et al., 2008) More details

$225 / 0.02 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NAA20, Polyclonal Antibody
Full Product Name
NAA20 Polyclonal Antibody
Product Gene Name
anti-NAA20 antibody
Product Synonym Names
NAT3; NAT5; NAT3P; NAT5P; dJ1002M8.1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Molecular Weight
178
OMIM
610833
3D Structure
ModBase 3D Structure for P61599
Species
Human
Immunogen
Recombinant protein of human NAA20
Calculated Molecular Weight
20kDa

Product Categories/Family for anti-NAA20 antibody

Polyclonal
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-NAA20 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-NAA20 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100

Western Blot (WB) of anti-NAA20 antibody

Western blot analysis of extracts of various cell lines, using NAA20 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Rabbit Anti-NAA20 Polyclonal Antibody – Cat# MBS9125312

Immunohistochemistry (IHC) of anti-NAA20 antibody

Immunohistochemistry of paraffin-embedded human oophoroma using NAA20 antibody at dilution of 1:100 (40x lens).
Rabbit Anti-NAA20 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9125312

Immunofluorescence (IF) of anti-NAA20 antibody

Immunofluorescence analysis of A549 cells using NAA20 antibody. Blue: DAPI for nuclear staining.
Rabbit Anti-NAA20 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9125312

Immunoprecipitation (IP) of anti-NAA20 antibody

Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 1ug NAA20 antibody. Western blot was performed from the immunoprecipitate using NAA20 antibody at a dilition of 1:1000.
Rabbit Anti-NAA20 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS9125312

Other Notes

Small volumes of anti-NAA20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NAA20 purchase

MBS9125312 is an antibody targeting NAA20. Also known as NAA20 Polyclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NAA20. It may not necessarily be applicable to this product.

NCBI GI #
47606438
NCBI GeneID
51126
NCBI Accession #
P61599.1
UniProt Primary Accession #
P61599
UniProt Secondary Accession #
Q5TFT7; Q9D7H8; Q9H0Y4; Q9NQH6; Q9Y6D2; A6NHA3; B2R4G4
UniProt Related Accession #
P61599
NCBI Official Full Name
N-alpha-acetyltransferase 20
NCBI Official Synonym Full Names
N(alpha)-acetyltransferase 20, NatB catalytic subunit
NCBI Official Symbol
NAA20
NCBI Official Synonym Symbols
NAT3; NAT5; NAT3P; NAT5P; dJ1002M8.1
UniProt Protein Name
N-alpha-acetyltransferase 20
UniProt Synonym Protein Names
Methionine N-acetyltransferase; N-acetyltransferase 5; N-terminal acetyltransferase B complex catalytic subunit NAA20; N-terminal acetyltransferase B complex catalytic subunit NAT5; NatB complex subunit NAT5; NatB catalytic subunit
Protein Family
N-alpha-acetyltransferase
UniProt Gene Name
NAA20
UniProt Synonym Gene Names
NAT5; NatB complex subunit NAT5
UniProt Entry Name
NAA20_HUMAN
NCBI Protein Information
N-alpha-acetyltransferase 20; natB catalytic subunit; natB complex subunit NAT5; N-acetyltransferase 3 homolog; methionine N-acetyltransferase; N-acetyltransferase 5 (GCN5-related, putative); N-terminal acetyltransferase complex ARD1 subunit; N-acetyltransferase 5 (ARD1 homolog, S. cerevisiae); N-alpha-acetyltransferase 20, NatB catalytic subunit; N-terminal acetyltransferase B complex catalytic subunit NAT5; N-terminal acetyltransferase B complex catalytic subunit NAA20; N-acetyltransferase 5, ARD1 subunit (arrest-defective 1, S. cerevisiae, homolog)

NCBI Summary for NAA20

NAT5 is a component of N-acetyltransferase complex B (NatB). Human NatB performs cotranslational N(alpha)-terminal acetylation of methionine residues when they are followed by asparagine (Starheim et al., 2008 [PubMed 18570629]).[supplied by OMIM, Apr 2009]

UniProt Comments for NAA20

NAA20: Catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp, Met-Glu, Met-Asn and Met-Gln. Proteins with cell cycle functions are overrepresented in the pool of NatB substrates. Required for maintaining the structure and function of actomyosin fibers and for proper cellular migration. Belongs to the acetyltransferase family. ARD1 subfamily.

Protein type: Secondary Metabolites Metabolism - limonene and pinene degradation; Acetyltransferase; Amino Acid Metabolism - phenylalanine; Lipid Metabolism - glycerophospholipid; Amino Acid Metabolism - tyrosine; EC 2.3.1.88

Chromosomal Location of Human Ortholog: 20p11.23

Cellular Component: cytoplasm; intracellular; nucleus

Molecular Function: peptide alpha-N-acetyltransferase activity

Biological Process: N-terminal peptidyl-methionine acetylation
Show more

Research Articles on NAA20

1. The human NatB complex composed of Naa20 (NAT3) and Naa25 (MDM20) N-terminally acetylates Met-Glu-, Met-Asp-, Met-Asn- and Met-Gln- N-termini, and is important for the structure and function of actomyosin fibers and for proper cellular migration.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NAA20 antibody

 Reference Product  PubMed Publications
 NAA25 antibody  >1 publications with NAA20 and NAA25

Pathways associated with anti-NAA20 antibody

 Products by Pathway  Pathway Diagram
 Metapathway Biotransformation antibodies  Metapathway Biotransformation Diagram
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000