• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PPP2R5D Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758718

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PPP2R5D, Polyclonal Antibody
Full Product Name
Anti-PPP2R5D Antibody Picoband
Product Gene Name
anti-PPP2R5D antibody
Product Synonym Names
semaphorin 3C; Semaphorin-3C; Semaphorin-E; Sema E; SEMA3C; SEMAE
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Expressed intensely in the heart, skeletal muscle, colon, small intestine, ovary, testis, and prostate. Faint expression ubiquitously among other organs, including brain.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
69,992 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (42952237..42980083). Location: 6p21.1
OMIM
601646
3D Structure
ModBase 3D Structure for Q14738
Immunogen
E coli-derived human PPP2R5D recombinant protein (Position: R91-L602).
Subcellular Localization
Secreted
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Binds to plexin family members and plays an important role in the regulation of developmental processes. Required for normal cardiovascular development during embryogenesis. Functions as attractant for growing axons, and thereby plays an important role in axon growth and axon guidance.

Product Categories/Family for anti-PPP2R5D antibody

Primary Antibodies; Cancer; Cell Biology; Oncoproteins/Suppressors; Protease Inhibitors; Proteolysis/Ubiquitin; Tumor Suppressors
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-PPP2R5D antibody
Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-PPP2R5D antibody
WB: 0.1-0.25ug/ml, Human, Mouse, Rat
FC/FACS (Fixed): 1-3ug/1x1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-PPP2R5D antibody

Figure 1. Western blot analysis of PPP2R5D using anti-PPP2R5D antibody (MBS1758718).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2R5D antigen affinity purified polyclonal antibody (#MBS1758718) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for PPP2R5D at approximately 74 kDa. The expected band size for PPP2R5D is at 70 kDa.
Rabbit Anti-PPP2R5D Polyclonal Antibody – Cat# MBS1758718

Flow Cytometry (FC/FACS) of anti-PPP2R5D antibody

Figure 2. Flow Cytometry analysis of SiHa cells using anti-PPP2R5D antibody (MBS1758718).
Overlay histogram showing SiHa cells stained with MBS1758718 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP2R5D Antibody (MBS1758718, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-PPP2R5D Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758718

Product References and Citations for anti-PPP2R5D antibody

1. Deciphering Developmental Disorders Study. Large-scale discovery of novel genetic causes of developmental disorders. Nature 519: 223-228, 2015.2. Houge, G., Haesen, D., Vissers, L. E. L. M., Mehta, S., Parker, M. J., Wright, M., Vogt, J., McKee, S., Tolmie, J. L., Cordeiro, N., Kleefstra, T., Willemsen, M. H., and 17 others. B56-delta-related protein phosphatase 2A dysfunction identified in patients with intellectual disability. J. Clin. Invest. 125: 3051-3062, 2015. 3. Louis, J. V., Martens, E., Borghgraef, P., Lambrecht, C., Sents, W., Longin, S., Zwaenepoel, K., Pijnenborg, R., Landrieu, I., Lippens, G., Ledermann, B., Gotz, J., Van Leuven, F., Goris, J., Janssens, V. Mice lacking phosphatase PP2A subunit PR61/B'delta (Ppp2r5d) develop spatially restricted tauopathy by deregulation of CDK5 and GSK3-beta. Proc. Nat. Acad. Sci. 108: 6957-6962, 2011.

Other Notes

Small volumes of anti-PPP2R5D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPP2R5D purchase

MBS1758718 is an antibody targeting PPP2R5D. Also known as Anti-PPP2R5D Antibody Picoband. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PPP2R5D. It may not necessarily be applicable to this product.

NCBI GI #
394582140
NCBI GeneID
5528
NCBI Accession #
NP_001257405.1
UniProt Primary Accession #
Q14738
UniProt Secondary Accession #
O00494; O00696; Q15171; Q5TC39; A8K3I9; B5BUA6
UniProt Related Accession #
Q14738
GenBank Nucleotide #
NM_001270476.1
NCBI Official Full Name
serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform isoform 4
NCBI Official Synonym Full Names
protein phosphatase 2, regulatory subunit B', delta
NCBI Official Symbol
PPP2R5D
NCBI Official Synonym Symbols
B56D
UniProt Protein Name
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
UniProt Synonym Protein Names
PP2A B subunit isoform B'-delta; PP2A B subunit isoform B56-delta; PP2A B subunit isoform PR61-delta; PP2A B subunit isoform R5-delta
Protein Family
Serine/threonine-protein phosphatase
UniProt Gene Name
PPP2R5D
UniProt Entry Name
2A5D_HUMAN
NCBI Protein Information
serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform; PP2A B subunit isoform B'-delta; PP2A B subunit isoform R5-delta; PP2A B subunit isoform B56-delta; PP2A B subunit isoform PR61-delta; PP2A, B subunit, B' delta isoform; PP2A

NCBI Summary for PPP2R5D

The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for PPP2R5D

PPP2R5D: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with SGOL1. By retinoic acid; in neuroblastoma cell lines. Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain. Belongs to the phosphatase 2A regulatory subunit B56 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleoplasm; protein phosphatase type 2A complex; cytosol; nucleus

Molecular Function: protein binding; protein phosphatase type 2A regulator activity; protein serine/threonine phosphatase activity

Biological Process: nervous system development; glycolysis; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; glucose metabolic process; pathogenesis; toll-like receptor 3 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; dephosphorylation; regulation of catalytic activity; carbohydrate metabolic process; energy reserve metabolic process; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway

Disease: Mental Retardation, Autosomal Dominant 35
Show more

Research Articles on PPP2R5D

1. Protein kinase A mediated activation of protein phosphatase 2A is enabled by PPP2R5D phosphorylation, which modulates the affinity of the protein phosphatase 2A holoenzyme to its physiological substrates.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PPP2R5D antibody

 Reference Product  PubMed Publications
 HAND1 antibody  >1 publications with PPP2R5D and HAND1

Pathways associated with anti-PPP2R5D antibody

 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alpha-synuclein Signaling Pathway antibodies  Alpha-synuclein Signaling Pathway Diagram
 Aurora A Signaling Pathway antibodies  Aurora A Signaling Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 Beta-catenin Phosphorylation Cascade Pathway antibodies  Beta-catenin Phosphorylation Cascade Pathway Diagram
 CTLA4 Inhibitory Signaling Pathway antibodies  CTLA4 Inhibitory Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram

Diseases associated with anti-PPP2R5D antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2 publications with PPP2R5D and Nervous System Diseases

Organs/Tissues associated with anti-PPP2R5D antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >4 publications with PPP2R5D and Brain
 Testis Antibodies  >3 publications with PPP2R5D and Testis
 Thyroid Antibodies  >2 publications with PPP2R5D and Thyroid
 Kidney Antibodies  >2 publications with PPP2R5D and Kidney
 Adipose Tissue Antibodies  >1 publications with PPP2R5D and Adipose Tissue
 Placenta Antibodies  >1 publications with PPP2R5D and Placenta
 Connective Tissue Antibodies  >1 publications with PPP2R5D and Connective Tissue
 Liver Antibodies  >1 publications with PPP2R5D and Liver
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000