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SLFN11 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9612274

Function: Inhibitor of DNA replication that promotes cell death in response to DNA damage (PubMed:22927417, PubMed:26658330, PubMed:29395061). Acts as a guardian of the genome by killing cells with defective replication (PubMed:29395061). Persistently blocks… More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SLFN11, Polyclonal Antibody
Full Product Name
SLFN11 Antibody
Product Gene Name
Product Synonym Names
Schlafen family member 11; SLFN11; SLFN8/9; SLN11_HUMAN
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Concentration
1mg/ml (lot specific)
Specificity
SLFN11 Antibody detects endogenous levels of total SLFN11.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
SLFN11 peptide (MBS9622218) is used for blocking the activity of SLFN11 antibody (MBS9612274)
Molecular Weight
Observed Molecular Weight: (Observed)102kD.
Predicted Molecular Weight: (Calculated)103kDa.
OMIM
614953
3D Structure
ModBase 3D Structure for Q7Z7L1
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human SLFN11, corresponding to a region within N-terminal amino acids.
Fragment
Fab fragment
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-SLFN11 antibody
ELISA (EIA)
Application Notes for anti-SLFN11 antibody
IF: 1:100-1:500
ICC: 1:100-1:500
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000

Western Blot (WB) of anti-SLFN11 antibody

Western blot analysis of extracts from Human pancreas, using SLFN11 Antibody. The lane on the left was treated with blocking peptide.
Rabbit Anti-SLFN11 Polyclonal Antibody – Cat# MBS9612274

Immunohistochemistry (IHC)-Paraffin of anti-SLFN11 antibody

MBS9612274 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-SLFN11 Polyclonal Antibody, Immunohistochemistry (IHC)-Paraffin – Cat# MBS9612274

Immunohistochemistry (IHC)-Paraffin of anti-SLFN11 antibody

MBS9612274 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-SLFN11 Polyclonal Antibody, Immunohistochemistry (IHC)-Paraffin – Cat# MBS9612274

Immunohistochemistry (IHC)-Paraffin of anti-SLFN11 antibody

MBS9612274 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-SLFN11 Polyclonal Antibody, Immunohistochemistry (IHC)-Paraffin – Cat# MBS9612274

Immunohistochemistry (IHC)-Paraffin of anti-SLFN11 antibody

MBS9612274 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-SLFN11 Polyclonal Antibody, Immunohistochemistry (IHC)-Paraffin – Cat# MBS9612274

Immunohistochemistry (IHC)-Paraffin of anti-SLFN11 antibody

MBS9612274 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
Rabbit Anti-SLFN11 Polyclonal Antibody, Immunohistochemistry (IHC)-Paraffin – Cat# MBS9612274

Immunofluorescence (IF)/Immunocytochemistry (ICC) of anti-SLFN11 antibody

MBS9612274 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612274 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.
The nuclear counter stain is DAPI(blue).
Rabbit Anti-SLFN11 Polyclonal Antibody, Immunofluorescence (IF)/Immunocytochemistry – Cat# MBS9612274

Other Notes

Small volumes of anti-SLFN11 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SLFN11 purchase

MBS9612274 is an antibody targeting SLFN11. Also known as SLFN11 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SLFN11. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
schlafen family member 11
NCBI Official Synonym Full Names
schlafen family member 11
NCBI Official Symbol
SLFN11
NCBI Official Synonym Symbols
SLFN8/9
UniProt Protein Name
Schlafen family member 11
Protein Family
UniProt Gene Name
SLFN11
UniProt Entry Name
SLN11_HUMAN
NCBI Protein Information
schlafen family member 11

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-SLFN11 antibody

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