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VPS25 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS7126707

MBS7126707 is an antibody targeting VPS25. Also known as VPS25 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$230 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
VPS25, Polyclonal Antibody
Full Product Name
VPS25 Antibody
Product Gene Name
Product Synonym Names
Vacuolar protein sorting 25 homolog (S. cerevisiae)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid; In Phosphate Buffered Saline, pH 7.4, 0.05% NaN3, and 40% Glycerol
Purity/Purification
Antigen Affinity Purification
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Molecular Weight
176
OMIM
610907
3D Structure
ModBase 3D Structure for Q9BRG1
Immunogen
Full length fusion protein
Immunogen Species
Human
Conjugate
Non-conjugated

Product Categories/Family for anti-VPS25 antibody

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-VPS25 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-VPS25 antibody
ELISA: 1:2000-1:5000
WB: 1:500-1:2000

SDS-PAGE of anti-VPS25 antibody

Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane: Human fetal liver tissue, Primary antibody: MBS7126707(VPS25 Antibody) at dilution 1/350 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
Rabbit Anti-VPS25 Polyclonal Antibody – Cat# MBS7126707

Other Notes

Small volumes of anti-VPS25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for VPS25. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Vacuolar protein-sorting-associated protein 25
NCBI Official Synonym Full Names
vacuolar protein sorting 25 homolog (S. cerevisiae)
NCBI Official Symbol
VPS25
NCBI Official Synonym Symbols
DERP9; EAP20; FAP20
UniProt Protein Name
Vacuolar protein-sorting-associated protein 25
UniProt Synonym Protein Names
Dermal papilla-derived protein 9; ELL-associated protein of 20 kDa; ESCRT-II complex subunit VPS25
UniProt Gene Name
VPS25
UniProt Synonym Gene Names
DERP9; EAP20; hVps25
UniProt Entry Name
VPS25_HUMAN
NCBI Protein Information
vacuolar protein-sorting-associated protein 25; ESCRT-II complex subunit VPS25; ELL-associated protein of 20 kDa; dermal papilla-derived protein 9

NCBI Summary for VPS25

This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Jul 2013]

UniProt Comments for VPS25

VPS25: Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses. Belongs to the VPS25 family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleoplasm; mitochondrion; nucleolus; endosome membrane; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; structural molecule activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; protein targeting to vacuole during ubiquitin-dependent protein catabolic process via the MVB pathway; endosome transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-VPS25 antibody

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