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AWAT1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9608957

Function: Acyltransferase that predominantly esterify long chain (wax) alcohols with acyl-CoA-derived fatty acids to produce wax esters. Wax esters are enriched in sebum, suggesting that it plays a central role in lipid metabolism in skin. Has a preference… More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
AWAT1, Polyclonal Antibody
Full Product Name
AWAT1 Antibody
Product Gene Name
Product Synonym Names
Acyl-CoA wax alcohol acyltransferase 1; Awat1; AWAT1_HUMAN; DGA; DGA2; Diacyl glycerol acyltransferase 2; Diacylglycerol acyltransferase 2; Diacylglycerol O acyltransferase 2 like 3; Diacylglycerol O-acyltransferase 2-like protein 3; Long-chain-alcohol O-fatty-acyltransferase 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
AWAT1 antibody detects endogenous levels of total AWAT1
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
AWAT1 peptide (MBS9627669) is used for blocking the activity of AWAT1 antibody (MBS9608957)
Molecular Weight
Observed: 37 kDa
Predicted: 38 kDa
3D Structure
ModBase 3D Structure for Q58HT5
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human AWAT1
Subcellular Location
Endoplasmic Reticulum Membrane.
Tissue Specificity
Predominantly expressed in skin, where it is limited to the sebaceous gland. Expressed in more mature, centrally located cells just before their rupture and sebum release. Also expressed in all tissues except spleen. Expressed at higher level in thymus, prostate and testis.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog
Similarity
Pig (83%), Bovine (83%), Horse (92%), Sheep (92%), Dog (92%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-AWAT1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-AWAT1 antibody
WB: 1:1000-3000
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-AWAT1 antibody

MBS9608957 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-AWAT1 Polyclonal Antibody – Cat# MBS9608957

Western Blot (WB) of anti-AWAT1 antibody

Western blot analysis of extracts from Rat brain, using AWAT1 Antibody.
Rabbit Anti-AWAT1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9608957

Western Blot (WB) of anti-AWAT1 antibody

Western blot analysis of AWAT1 using NIH-3T3 whole cell lysates
Rabbit Anti-AWAT1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9608957

Other Notes

Small volumes of anti-AWAT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AWAT1 purchase

MBS9608957 is an antibody targeting AWAT1. Also known as AWAT1 Antibody. Reactive in Human, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for AWAT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
acyl-CoA wax alcohol acyltransferase 1
NCBI Official Synonym Full Names
acyl-CoA wax alcohol acyltransferase 1
NCBI Official Symbol
AWAT1
NCBI Official Synonym Symbols
DGA2; DGAT2L3
UniProt Protein Name
Acyl-CoA wax alcohol acyltransferase 1
UniProt Synonym Protein Names
Diacylglycerol O-acyltransferase 2-like protein 3; Diacylglycerol acyltransferase 2; Long-chain-alcohol O-fatty-acyltransferase 1
UniProt Gene Name
AWAT1
UniProt Synonym Gene Names
DGA2; DGAT2L3
NCBI Protein Information
acyl-CoA wax alcohol acyltransferase 1

NCBI Summary for AWAT1

The protein encoded by this gene belongs to the diacylglycerol acyltransferase family. It esterifies long chain (wax) alcohols with acyl-CoA-derived fatty acids to produce wax esters. Wax esters are enriched in sebum, suggesting that this enzyme plays a central role in lipid metabolism in skin. Consistent with this observation, this protein is predominantly expressed in the sebaceous gland of the skin. [provided by RefSeq, Sep 2009]
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UniProt Comments for AWAT1

Acyltransferase that predominantly esterify long chain (wax) alcohols with acyl-CoA-derived fatty acids to produce wax esters. Wax esters are enriched in sebum, suggesting that it plays a central role in lipid metabolism in skin. Has a preference for arachidyl alcohol as well as decyl alcohol, demonstrating its relatively poor activity using saturated long chain alcohols (C16, C18, and C20).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-AWAT1 antibody

Organs/Tissues associated with anti-AWAT1 antibody

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >1 publications with AWAT1 and Skin
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