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RING1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1758237

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring… More details

$210 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
RING1, Polyclonal Antibody
Full Product Name
Anti-RING1 Antibody Picoband
Product Gene Name
anti-RING1 antibody
Product Synonym Names
Serine/threonine-protein kinase AtPK1/AtPK6; 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Purity/Purification
Immunogen affinity purified.
Specificity
Expressed in all tissues.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
Molecular Weight
406
OMIM
602045
3D Structure
ModBase 3D Structure for Q06587
Immunogen
E coli-derived human RING1 recombinant protein (Position: N6-K387). Human RING1 shares 96.3% and 96.6% amino acid (aa) sequence identity with mouse and rat RING1, respectively.
Subcellular Localization
Cytoplasm, Nucleus
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross reactivity with other proteins.
Protein Function
Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.

Product Categories/Family for anti-RING1 antibody

Primary Antibodies; Signal Transduction; Signaling Pathway; Small G Proteins
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-RING1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes for anti-RING1 antibody
WB: 0.5-1ug/ml, Human, Rat
ICC/IF: 5ug/ml, Human
FC/FACS (Fixed): 1-3ug/1x106 cells, Human
ELISA: 0.1-0.5ug/ml

Western Blot (WB) of anti-RING1 antibody

Figure 1. Western blot analysis of RING1 using anti-RING1 antibody (MBS1758237).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RING1 antigen affinity purified polyclonal antibody (#MBS1758237) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (#MBS176460) with Tanon 5200 system. A specific band was detected for RING1 at approximately 50 kDa. The expected band size for RING1 is at 43 kDa.
Rabbit Anti-RING1 Polyclonal Antibody – Cat# MBS1758237

Immunofluorescence (IF) of anti-RING1 antibody

Figure 2. IF analysis of RING1 using anti-RING1 antibody (MBS1758237) and anti-Beta Tubulin antibody (M01857-3).
RING1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (#MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-RING1 Antibody (MBS1758237) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and FITC Conjugated Goat Anti-Mouse IgG (BA1101) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Rabbit Anti-RING1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS1758237

Flow Cytometry (FC/FACS) of anti-RING1 antibody

Figure 3. Flow Cytometry analysis of JK cells using anti-RING1 antibody (MBS1758237).
Overlay histogram showing JK cells stained with MBS1758237 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RING1 Antibody (MBS1758237, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (MBS177405, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Rabbit Anti-RING1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1758237

Product References and Citations for anti-RING1 antibody

1. de Napoles, M., Mermoud, J. E., Wakao, R., Tang, Y. A., Endoh, M., Appanah, R., Nesterova, T. B., Silva, J., Otte, A. P., Vidal, M., Koseki, H., Brockdorff, N. Polycomb group proteins Ring1A/B link ubiquitylation of histone H2A to heritable gene silencing and X inactivation. Dev. Cell 7: 663-676, 2004.2. Kikuti, Y. Y., Tamiya, G., Ando, A., Chen, L., Kimura, M., Ferreira, E., Tsuji, K., Trowsdale, J., Inoko, H. Physical mapping 220 kb centromeric of the human MHC and DNA sequence analysis of the 43-kb segment including the RING1, HKE6, and HKE4 genes. Genomics 42: 422-435, 1997.3. Lovering, R., Hanson, I. M., Borden, K. L. B., Martin, S., O'Reilly, N. J., Evan, G. I., Rahman, D., Pappin, D. J. C., Trowsdale, J., Freemont, P. S. Identification and preliminary characterization of a protein motif related to the zinc finger. Proc. Nat. Acad. Sci. 90: 2112-2116, 1993.

Other Notes

Small volumes of anti-RING1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RING1 purchase

MBS1758237 is an antibody targeting RING1. Also known as Anti-RING1 Antibody Picoband. Reactive in Human, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RING1. It may not necessarily be applicable to this product.

NCBI GI #
90110053
NCBI GeneID
6015
NCBI Accession #
Q06587.2
UniProt Primary Accession #
Q06587
UniProt Secondary Accession #
Q5JP96; Q5SQW2; Q86V19; A8JZZ0
UniProt Related Accession #
Q06587
NCBI Official Full Name
E3 ubiquitin-protein ligase RING1
NCBI Official Synonym Full Names
ring finger protein 1
NCBI Official Symbol
RING1
NCBI Official Synonym Symbols
RNF1; RING1A
UniProt Protein Name
E3 ubiquitin-protein ligase RING1
UniProt Synonym Protein Names
Polycomb complex protein RING1; RING finger protein 1; Really interesting new gene 1 protein
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
RING1
UniProt Synonym Gene Names
RNF1
UniProt Entry Name
RING1_HUMAN
NCBI Protein Information
E3 ubiquitin-protein ligase RING1; polycomb complex protein RING1; really interesting new gene 1 protein

NCBI Summary for RING1

This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. [provided by RefSeq, Jul 2008]
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UniProt Comments for RING1

RING1: Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity. Component of chromatin-associated Polycomb (PcG) complexes. Interacts with BMI1. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2/RING2 MBLR, L3MBTL2 and YAF2. Interacts with CBX2 and PCGF6. Component of a PRC1-like complex. Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of RYBP, PCGF1, BCOR and RNF2/RING2. Interacts with PCGF2, RNF2; CBX6, CBX7 and CBX8. Interacts with PHC2. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; Transcription factor; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; sex chromatin; cytoplasm; nuclear speck; PcG protein complex; nucleus

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; chromatin binding; ligase activity

Biological Process: anterior/posterior pattern formation; camera-type eye morphogenesis; transcription, DNA-dependent; negative regulation of transcription, DNA-dependent
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Research Articles on RING1

1. HSCARG interacts with RING1 and inhibits RING1 ubiquitination.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RING1 antibody

 Reference Product  PubMed Publications
 BMI1 antibody  >37 publications with RING1 and BMI1
 RNF2 antibody  >29 publications with RING1 and RNF2
 EZH2 antibody  >14 publications with RING1 and EZH2
 CBX4 antibody  >11 publications with RING1 and CBX4
 CBX8 antibody  >7 publications with RING1 and CBX8
 CBX2 antibody  >6 publications with RING1 and CBX2
 CBX7 antibody  >5 publications with RING1 and CBX7
 PHC1 antibody  >4 publications with RING1 and PHC1
 PHC2 antibody  >2 publications with RING1 and PHC2

Pathways associated with anti-RING1 antibody

 Products by Pathway  Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram
 Oxidative Stress Induced Senescence Pathway antibodies  Oxidative Stress Induced Senescence Pathway Diagram

Diseases associated with anti-RING1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >10 publications with RING1 and Nervous System Diseases
 Cell Transformation, Neoplastic Antibodies  >4 publications with RING1 and Cell Transformation, Neoplastic
 Inflammation Antibodies  >3 publications with RING1 and Inflammation
 Cardiovascular Diseases Antibodies  >3 publications with RING1 and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >2 publications with RING1 and Breast Neoplasms
 Liver Neoplasms Antibodies  >2 publications with RING1 and Liver Neoplasms
 Atrophy Antibodies  >2 publications with RING1 and Atrophy
 Necrosis Antibodies  >2 publications with RING1 and Necrosis
 Hyperplasia Antibodies  >1 publications with RING1 and Hyperplasia
 Carcinoma, Hepatocellular Antibodies  >1 publications with RING1 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-RING1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >13 publications with RING1 and Blood
 Brain Antibodies  >7 publications with RING1 and Brain
 Bone Antibodies  >6 publications with RING1 and Bone
 Lung Antibodies  >5 publications with RING1 and Lung
 Embryonic Tissue Antibodies  >5 publications with RING1 and Embryonic Tissue
 Kidney Antibodies  >4 publications with RING1 and Kidney
 Eye Antibodies  >4 publications with RING1 and Eye
 Prostate Antibodies  >4 publications with RING1 and Prostate
 Placenta Antibodies  >4 publications with RING1 and Placenta
 Liver Antibodies  >4 publications with RING1 and Liver
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